首页|Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system

Extraction of fucoxanthin from Chaetoceros calcitrans by electropermeabilization-assisted liquid biphasic flotation system

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  • NSTL
  • Elsevier
Electropermeabilization-assisted liquid biphasic flotation (LBF) system is an emerging extraction system facilitated by the adsorptive bubble separation and the electroporation in an aqueous two-phase medium. This integrative extraction system is well suited for the direct recovery of intracellular biocompounds from cells without the needs of discrete steps of mid-or down-stream bioprocessing. The potential of electropermeabilization-assisted LBF system in bioseparation was demonstrated in this study by using the diatom Chaetoceros calcitrans ( C. calcitrans) as the source of fucoxanthin, which is a promising an-tioxidant highly demanded by food and pharmaceutical industries. The extraction performances of LBF and electropermeabilization-assisted LBF system were comprehensively evaluated under the optimal op-erating conditions. Comparatively, the optimized LBF and electropermeabilization-assisted LBF systems achieved the satisfactory yields of fucoxanthin, i.e., 14.78 mg/g and 16.09 mg/g, respectively. The good recovery of fucoxanthin using electropermeabilization-assisted LBF system could be attributed to the higher release of fucoxanthin from the electrotreated C. calcitrans, allowing a higher partition of fucox-anthin to the top phase of LBF system (236.72 as compared to 152.15 from LBF system). In addition, the fucoxanthin extracted suing both methods exhibit satisfactory antioxidant activities. The application of electropermeabilization-assisted LBF system in the extraction of fucoxanthin is a greener and highly ef-ficient bioprocessing route which can be prospectively extended to other biocompound extraction from microalgal sources. (c) 2022 Elsevier B.V. All rights reserved.

Chaetoceros calcitransFucoxanthinElectropermeabilization-assistedExtractionLiquid biphasic flotationAQUEOUS 2-PHASE FLOTATIONPULSED ELECTRIC-FIELDANTIOXIDANT ACTIVITYBETACYANINS EXTRACTIONHYLOCEREUS-POLYRHIZUSINTEGRATION PROCESSELECTROPORATIONSEPARATIONRECOVERYSOLVENT

Khoo, Kuan Shiong、Ooi, Chien Wei、Chew, Kit Wayne、Chia, Shir Reen、Foo, Su Chern、Ng, Hui Suan、Show, Pau Loke

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UCSI Univ

Monash Univ Malaysia

Xiamen Univ Malaysia

Univ Tenaga Nasl UNITEN

Univ Nottingham Malaysia

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2022

10.1016/j.chroma.2022.462915

Journal of chromatography

Journal of chromatography

ISSN：0021-9673
年,卷(期)：2022.1668
  • 6
  • 42