首页|Expression and functional characterization of peptidoglycan recognition protein-S6 involved in antibacterial responses in the razor clam Sinonovacula constricta

Expression and functional characterization of peptidoglycan recognition protein-S6 involved in antibacterial responses in the razor clam Sinonovacula constricta

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  • NSTL
  • Elsevier
It has been recognized that peptidoglycan recognition proteins (PGRPs), structurally conserved molecules, play crucial roles in the innate immunity of invertebrate. However, few studies have been taken to explore their potential functions. In this study, a novel PGRP from the razor clam Sinonovacula constrict designated as ScPGRPS6 was identified and characterized. The open reading frame (ORF) of ScPGRP-S6 was 666 bp in length, encoding a protein of 221 amino acid with a signal peptide (1-30) and a typical PGRP domain (39-187). The sequence alignment combined with phylogenetic analysis collectively confirmed that ScPGRP-S6 was a novel member belonging to PGRP-S family. The mRNA transcript of ScPGRP-S6 in the hepatopancreases was significantly upregulated after peptidoglycan (PGN) stimulation, while it was moderately up-regulated after lipopolysaccharide (LPS) stimulation. The result of immunofluorescence detection demonstrated that the positive signal enhanced obviously after Vibrio parahaemolyticus challenge. Notably, the recombinant protein of ScPGRP-S6 (designed as rScPGRP-S6) exhibited high agglutination activity towards V. parahaemolyticus but weak to Staphylococcus aureus. Furthermore, rScPGRP-S6 showed strong amidase and antibacterial activity in the presence of Zn2+. Collectively, our results manifested that ScPGRP-S6 could act as a scavenger in the innate immune response of S. constricta.

Sinonovacula constrictaPeptidoglycan recognition proteinPAMPs stimulationAmidase activityAntibacterial activityMESSENGER-RNA EXPRESSIONFRESH-WATER MUSSELL-ALANINE AMIDASEMOLECULAR-CLONINGINNATE IMMUNITYPACIFIC OYSTERPGRP GENESDROSOPHILAIDENTIFICATIONLC

Meng, Yiping、Dai, Wenfang、Lin, Zhihua、Zhang, Weiwei、Dong, Yinghui

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Zhejiang Wanli Univ

Ningbo Univ

2022

10.1016/j.dci.2021.104331

Developmental and Comparative Immunology

Developmental and Comparative Immunology

SCI
ISSN：0145-305X
年,卷(期)：2022.129
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