Experimental confirmation of antimicrobial effects of GdYVO4:Eu3+ nanoparticles

Kavok, Nataliya ¹Maksimchuk, Pavel ¹Dizge, Nadir ²Ocakoglu, Kasim ³Gonca, Serpil ⁴Ozdemir, Sadin ⁵Yefimova, Svetlana ¹Tkachenko, Anton ⁶Onishchenko, Anatolii ⁶Klochkov, Vladimir¹

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作者信息

1. Inst Scintillat Mat,Natl Acad Sci Ukraine
2. Dept Environm Engn,Mersin Univ
3. Fac Engn,Tarsus Univ
4. Fac Pharm,Mersin Univ
5. Food Proc Programme,Mersin Univ
6. Res Inst Expt & Clin Med,Kharkiv Natl Med Univ
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Abstract

Nanotechnology can be applied to design antibacterial agents to combat antibiotic resistance. The aim of the present study was to assess the antimicrobial effects and cytotoxicity of GdYVO4:Eu3+ nanoparticles (NPs). Biofilm inhibition activity, antimicrobial activity, bacterial viability inhibition and DNA cleavage activity of GdYVO4:Eu3+ NPs were studied. In addition, the impact of GdYVO4:Eu3+ NPs on the mitochondrial membrane potential (Delta psi(M)) of host immune cells and, hence, their apoptosis was analyzed by JC-1 staining using flow cytometry. GdYVO4:Eu3+ NPs demonstrated good antimicrobial, cell viability inhibition and DNA cleavage activities. In addition, GdYVO4:Eu3+ NPs showed good biofilm inhibition activity against S. aureus and P. aeruginosa and inhibition percentages were 89.15% and 79.54%, respectively. However, GdYVO4:Eu3+ NPs promoted mitochondrial depolarization and apoptosis of leukocytes at high concentrations. GdYVO4:Eu3+ nanoparticles are promising antibacterial agents. However, more efforts should be exerted to ensure their safety.

Key words

JC-1/mitochondrial membrane potential/DNA cleavage/biofilm inhibition activity/antimicrobial activity/bacterial viability/nanoparticles/ANTIBIOTIC-RESISTANCE/SILVER NANOPARTICLES/BIOFILM FORMATION/ANTIBACTERIAL/GENERATION/JC-1/GD

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出版年

2021

Drug development and industrial pharmacy

SCI

ISSN：0363-9045

被引量1

参考文献量45

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