首页| Bacterial community dynamics during MEOR biostimulation of an reservoir in sumatera Indonesia
Bacterial community dynamics during MEOR biostimulation of an reservoir in sumatera Indonesia
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Biostimulation is a method of microbial enhanced oil recovery (MEOR) by injecting nutrients into an oil well to stimulate the growth of indigenous bacteria and their activities dealing with the increasing of oil recovery. This study aimed to obtain an optimum nutrient formulation at laboratory scale to be applied for MEOR field biostimulation pilot project. Field monitoring of chemical and bacterial composition in oil well was also carried out up to twelve weeks after biostimulation. The ojl composition was analyzed by GC-MS and column chromatography. Bacterial community analysis was performed using 16S rRNA gene as the biomarker, which was sequenced by Illumina Miseq Sequencing. Based on Response Surface Methodology (RSM), the optimum nutrient concentrations that increase bacterial growth and decrease oil viscosity were molasses 5% (w/v), NPK 0.5% (w/ v), and DAP 0.5% (w/v). The relative abundance of the bacteria and the community composition constantly changed during the monitoring process. Injection of molasses stimulated the growth of the hydrocarbonoclastic bacteria until the end of monitoring period.
Dea Indriani Astuti、Isty Adhitya Purwasena、Neil Priharto
展开 >
Department of Microbiology, School of Life Sciences and Technology, Institut Teknologi Bandung, Ganesha Street no 10, Bandung, West Java, 40132, Indonesia
Department of Petroleum Engineering, Faculty of Mining and Petroleum Engineering, Institut Teknologi Bandung, Ganesha Street no 10, Bandung, West Java, 40132, Indonesia
NSTL
