首页|Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants

Differential proteome between ejaculate and epididymal sperm represents a key factor for sperm freezability in wild small ruminants

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  • NSTL
  • Elsevier
Epididymal sperm shows higher cryoresistance than ejaculated sperm. Although the sperm proteome seems to affect cell cryoresistance, studies aiming at identifying proteins involved in sperm freezing-tolerance are scarce. The aims of this study were to investigate differences of sperm freezability and proteome between epididymal and ejaculated sperm in three mountain ungulates: Iberian ibex, Mouflon and Chamois. Sperm samples were cryopreserved in straws by slow freezing. Tandem mass tag-labeled peptides from sperm samples were analyzed by high performance liquid chromatography coupled to a mass spectrometer in three technical replicates. The statistical analysis was done using the moderated t-test of the R package limma. Differences of freezability between both types of sperm were associated with differences of the proteome. Overall, epididymal sperm showed higher freezability than ejaculated sperm. Between 1490 and 1883 proteins were quantified in each species and type of sperm sample. Cross species comparisons revealed a total of 76 proteins that were more abundant in epididymal than in ejaculated sperm in the three species of study whereas 3 proteins were more abundant in ejaculated than epididymal sperm in the three species of study (adjusted P 0.5). Many of the proteins that were associated with higher cryoresistance are involved in stress response and redox homeostasis. In conclusion, marked changes of sperm proteome were detected between epididymal and ejaculated sperm. This work contributes to update the sperm proteome of small ruminants and to identify candidate markers of sperm freezability.

CryopreservationGermplasm bankGoatMass spectrometryProteomicsSheep

Martinez-Fresneda, Lucia、Sylvester, Marc、Shakeri, Farhad、Bunes, Andreas、Del Pozo, Juan C.、Garcia-Vazquez, Francisco A.、Neuhoff, Christiane、Tesfaye, Dawit、Schellander, Karl、Santiago-Moreno, Julian

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Spanish Natl Inst Agr & Food Res & Technol INIA, Dept Anim Reprod, Avda Puerta Hierro Km 5-9

Univ Bonn, Inst Biochem & Mol Biol, Fac Med, Nussallee 11, D-53115 Bonn, Germany

Univ Bonn, Inst Med Biometry Informat & Epidemiol, Fac Med, Venusberg Campus 1, D-53127 Bonn

Univ Politecn Madrid, Ctr Biotechnol & Plant Genom, Natl Inst Agr & Food Res & Technol UPM INIA

Univ Murcia, Fac Vet Sci Int Excellence Campus Higher Educ & R, Dept Physiol, Campus Espinardo

Univ Bonn, Inst Anim Sci, Dept Anim Breeding & Husb, Endenicher Allee 15, D-53115 Bonn, Germany

Colorado State Univ, Dept Biomed Sci, Anim Reprod & Biotechnol Lab, 3105 Rampart Rd, Ft Collins, CO

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2021

10.1016/j.cryobiol.2021.01.012

Cryobiology

Cryobiology

SCI
ISSN：0011-2240
年,卷(期)：2021.99
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