首页|Retention of substituted coumarins using immobilized artificial membrane (IAM) chromatography: a comparative study with n-octanol partitioning and reversed-phase HPLC and TLC.
Retention of substituted coumarins using immobilized artificial membrane (IAM) chromatography: a comparative study with n-octanol partitioning and reversed-phase HPLC and TLC.
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The chromatographic indices log k(wIAM) have been determined by HPLC on an immobilized artificial membrane column for a set of coumarin derivatives. The investigated compounds contain substituted amidoximes, or substituted heterocycles directly attached to the coumarinic skeleton. The log k(wIAM) values were compared to previously reported data of octanol-water partition coefficients and extrapolated capacity factors determined by reversed phase HPLC and TLC. The log k(wIAM) values of the investigated compounds were found to be comparable with the corresponding log P values, although they constitute a lower lipophilicity scale due to the reduced hydrophobic environment of the IAM stationary phase. These features were exemplified in their interrelationship with a slope close to unity and a large negative intercept. In contrast their comparison with the corresponding HPLC and RP-TLC capacity factors revealed differences in the retention mechanism reflected in slopes lower than unity, which were postulated to be due to secondary interactions under the reversed phase chromatographic conditions. However, conformational effects in the molecular structures of the coumarin derivatives were found to have a similar impact in their affinity for the IAM and octadecyl silane stationary phases, while they did not affect their octanol-water partitioning.
Coumarins香豆素类
Vrakas D、Hadjipavlou Litina D、Tsantili Kakoulidou A
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Department of Pharmaceutical Chemistry, School of Pharmacy, University of Athens, Panepistimiopolis, Zografou, Athens 157 71, Greece.
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