首页|Donor manipulation for constructing a pH sensing thermally activated delayed fluorescent probe to detect alkaliphiles
Donor manipulation for constructing a pH sensing thermally activated delayed fluorescent probe to detect alkaliphiles
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NSTL
Elsevier
? 2022 Elsevier B.V.pH homeostasis is essential for alkaliphiles, given their widespread use in biotechnological applications. However, quantitative monitoring of alkaline pH in alkaliphiles remains challenging. Here, we synthesized for the first time, a thermally activated delayed fluorescent (TADF) pH probe: NI-D-OH. Our probe exhibits a good linear relationship between fluorescence intensity and pH in the neutral to alkaline range (pH 7.0–8.6), as well as long-lived TADF emission. We further show that NI-D-OH can be used to monitor intracellular pH in living organisms, and evaluate the effect of Na+ on the pH homeostasis, demonstrating the potential for alkaline pH monitoring and time-resolved fluorescence imaging.
Liu Q.、Yang M.、Han X.、Hu H.-Y.、Zhang Q.、Xu Y.、Nazare M.、Meng X.
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State Key Laboratory of Bioactive Substances and Function of Natural Medicine Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation Institute of Materia Medica Chinese Academy of Medical Sciences & Peking Union Medical College
School of Pharmaceutical Engineering and Key Laboratory of Structure-Based Drug Design & Discovery (Ministry of Education) Shenyang Pharmaceutical University
Medicinal Chemistry Leibniz-Forschungsinstitut für Molekulare Pharmakologie Berlin
NSTL
Elsevier
