首页|Yam (Dioscorea cayennensis) protein concentrate: Production, characterization and in vitro evaluation of digestibility

Yam (Dioscorea cayennensis) protein concentrate: Production, characterization and in vitro evaluation of digestibility

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  • NSTL
  • Elsevier
This study aimed to produce a yam protein concentrate (YPC), Dioscorea cayennensis, to evaluate its protein digestibility and the peptide profile generated through simulated gastrointestinal digestion (GID). After concentration, the YPC that resulted in 64.0% protein was obtained. Simulated GID and free amino acids analysis were performed to evaluate its digestibility. For the peptide profile was evaluated by means of structure, molecular weight (MW) (nano-LC-ESI-MS/MS and SEC-FPLC), and hydrophobicity (RP-HPLC). Roughly 71.0% of peptides showed MW < 2 kDa after GID. The peptide sequences KQAVNENAINNARPLQPTN, GRSDPFLSDL, and KNEINAGVVDPNQLQF were identified in the gastric digestion. These sequences were further digested in the intestinal phase, yielding the NAINNARPL, GRSDPF, and VVDPN peptides. The GID process generated molecules with smaller MW, with consequent exposure of ionizable and functional groups, potentializing the yam proteins bioactive capacity. The peptides obtained after the digestion should be explored in future studies with the objective of understanding the bioactive potential of these molecules for human health.

Simulated gastrointestinal digestion in vitroDioscorea cayennensistuberNano-LC-ESI-MS/MS

do Nascimento, Edilza Silva、Caju de Oliveira, Julia Mariano、Jocelino Gomes de Lacerda, Jose Thalles、Montenegro, Samara Batista、Caetano-Silva, Maria Elisa、Dias, Meriellen、Mendes, Maria Anita、Juliano, Maria Aparecida、Gadelha, Tatiane Santi、Bertoldo Pacheco, Maria Teresa、de Almeida Gadelha, Carlos Alberto

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Univ Fed Paraiba, Technol Ctr, Dept Food Engn, Postgrad Program Food Sci & Technol, Joao Pessoa, Paraiba, Brazil

Univ Fed Paraiba, Dept Mol Biol, Lab Struct Prote, BR-58051900 Joao Pessoa, Paraiba, Brazil

Univ Fed Sao Paulo, Dept Biophys, Sao Paulo, SP, Brazil

Ctr Food Sci & Qual, Inst Food Technol, Campinas, SP, Brazil

Univ Sao Paulo, Dept Chem Engn, Dempster MS Lab, Sao Paulo, SP, Brazil

Univ Fed Paraiba, Dept Mol Biol, Lab Genet Biochem & Radiol, Joao Pessoa, Paraiba, Brazil

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2021

10.1016/j.lwt.2020.110771

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN：0023-6438
年,卷(期)：2021.140
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