首页|Lactic microbiota of the minas artisanal cheese produced in the serro region, Minas Gerais, Brazil

Lactic microbiota of the minas artisanal cheese produced in the serro region, Minas Gerais, Brazil

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The lactic microbiota of the Minas artisanal cheese (MAC) produced in the Serro region was characterized by culture-dependent and -independent methods. Samples of Serro MAC (n = 55) were obtained and plated for lactic acid bacteria (LAB) enumeration. Isolates (n = 176) were subsequently identified by 16S rRNA sequencing as lactobacilli, Lactococcus lactis and Enterococcus sp. LAB counts and the distribution of identified species did not differ by producer city, producer farm size, starter culture and time of production (P > 0.05). DNA from samples was subjected to high-throughput 16S rRNA sequencing and L. lactis was the predominant species in all samples. Alpha-diversity indices (species number, diversity, richness) and beta-diversity analysis did not differ between samples (P > 0.05). Core microbiota for all cheese samples included L. lactis, Streptococcus salivarius, S. thermophilus and Acinetobacter johnsonii. Here, we were able to show that lactobacilli and L. lactis are the dominant lactic groups and species in the Serro MAC, supporting the establishment of a typical microbial signature for this cheese.

Brazilian artisanal cheeseLactic bacteria diversityHigh-throughput sequencing

Nero, Luis Augusto、Andretta, Milimani、Almeida, Thaiza T.、Ferreira, Leticia R.、Camargo, Anderson C.、Yamatogi, Ricardo S.、Carvalho, Antonio F.、Call, Douglas R.

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Univ Fed Vicosa, Dept Vet, InsPOA Lab Inspecao Prod Origem Anim, Campus Univ, BR-36570900 Vicosa, MG, Brazil

Univ Fed Vicosa, Dept Tecnol Alimentos Inovaleite, Lab Ciencia & Tecnol Leite & Derivados, Campus Univ, BR-36570900 Vicosa, MG, Brazil

Washington State Univ, Paul G Allen Sch Global Anim Hlth, 240 SE Ott Rd POB 647090, Pullman, WA 99164 USA

2021

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN:0023-6438
年,卷(期):2021.148
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