Plant Physiology and Biochemistry2022，Vol.1868.DOI:10.1016/j.plaphy.2022.06.012

Influence of the sulfate content of the exopolysaccharides from Porphyridium sordidum on their elicitor activities on date palm vitroplants

Abdelkafi, Slim Fendri, Imen El Modafar, Cherkaoui Drira, Marwa Elleuch, Jihen Hadjkacem, Farah Hentati, Faiez Drira, Riadh Pierre, Guillaume Gardarin, Christine Delattre, Cedric El Alaoui-Talibi, Zainab Michaud, Philippe
Plant Physiology and Biochemistry2022，Vol.1868.DOI:10.1016/j.plaphy.2022.06.012
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  • NSTL
  • Elsevier

Influence of the sulfate content of the exopolysaccharides from Porphyridium sordidum on their elicitor activities on date palm vitroplants

Abdelkafi, Slim 1Fendri, Imen 1El Modafar, Cherkaoui 2Drira, Marwa 1Elleuch, Jihen 1Hadjkacem, Farah 1Hentati, Faiez 3Drira, Riadh 1Pierre, Guillaume 4Gardarin, Christine 4Delattre, Cedric 4El Alaoui-Talibi, Zainab 2Michaud, Philippe4
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作者信息

  • 1. Univ Sfax
  • 2. Univ Cadi Ayyad
  • 3. Univ Lorraine
  • 4. Univ Clermont Auvergne
  • 折叠

Abstract

Given the increasing interest that is being paid to polysaccharides derived from algae as plant natural defense stimulators, the degree of sulfation of exopolysaccharides produced by P. sordidum for inducing defense re-sponses in date palm vitroplants was investigated. Firstly, the culture parameters of P. sordidum were optimized to maximize the amount of sulfate in EPS using a Box-Behnken experimental design and the elicitor effects of two EPS which differ in the sulfation degrees were compared. Results demonstrated that the concentrations of NaCl, NaNO3 and MgSO4 set at 28, 0.54 and 16.31 g/L, respectively yielded the best sulfate contents. To elucidate defense-inducing activities in date palm vitroplants, EPS with the highest sulfate content (EPS1) were prepared for comparison with those obtained under standard conditions (EPS0). A fucoidan extracted from Cystoseira compressa was used as positive control and MgSO4 as negative control. Both EPS and the fucoidan displayed H2O2 accumulation and expression of PR1, SOD, PAL and WRKY genes. Interestingly, EPS1 was significantly more bioactive than EPS0 and the fucoidan suggesting that the elicitor activity is positively correlated with the sulfate groups content of this polysaccharide.

Key words

Polysaccharides/Sulfate/Elicitor/Date palm vitroplants/P. sordidum/ANTIOXIDANT ACTIVITY/QUANTITATIVE PCR/POLYSACCHARIDES/RESISTANCE/PLANTS

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出版年

2022
Plant Physiology and Biochemistry

Plant Physiology and Biochemistry

SCI
ISSN：0981-9428
被引量1
参考文献量55
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