首页|UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength
UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength
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NSTL
Elsevier
Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 sus-pensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, pro-tein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260-and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310-and 365-nm UV-LEDs. Meanwhile, 260-and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260-and 280-nm UV-LEDs, 310-and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation.
Light-emitting diodeUltravioletHerpes simplex virus type 1DNA photoproducsViral lipidsULTRAVIOLET-LIGHTDISINFECTION PERFORMANCELIPID-PEROXIDATIONCYCLOBUTANE DIMERINACTIVATIONABSORPTIONREPAIRENTRYDNAMICROORGANISMS
NSTL
Elsevier
