International journal of peptide research and therapeutics.2022，Vol.28Issue(5) ：12.DOI:10.1007/s10989-022-10442-w

Production Optimization and In Vitro Evaluation of Anti-proliferative, Anti-oxidant and Anti-inflammatory Potential of the Antibacterial Peptide MFAP9

Raghavan, Rekha Mol Kollakalnaduvil Pannippara, Manzur Ali Kesav, Sapna Mathew, Abraham Bhat, Sarita G. Rafeeq, C. M. Elyas, K. K.
International journal of peptide research and therapeutics.2022，Vol.28Issue(5) ：12.DOI:10.1007/s10989-022-10442-w
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Production Optimization and In Vitro Evaluation of Anti-proliferative, Anti-oxidant and Anti-inflammatory Potential of the Antibacterial Peptide MFAP9

Raghavan, Rekha Mol Kollakalnaduvil 1Pannippara, Manzur Ali 1Kesav, Sapna 1Mathew, Abraham 1Bhat, Sarita G. 1Rafeeq, C. M. 2Elyas, K. K.3
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作者信息

  • 1. Cochin University Science & Technology,Cochin Univ Sci & Technol
  • 2. Dept Biotechnol,MES Coll Marampally
  • 3. Dept Biotechnol,Calicut Univ
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Abstract

Peptides are important natural bioactive substances obtained from various sources such as microbes, plants and animals. The present study addressed the optimization and physicochemical characterization of the antibacterial peptide Marine Fungal Antibacterial Peptide 9 (MFAP9) produced by the marine fungus Aspergillus fumigatus BTMF9 and the in vitro evaluation of its antiproliferative, antioxidant and anti-inflammatory potential. Antiproliferative activity was evaluated in A549 lung carcinoma and normal L929 fibroblast cell lines using the MTT assay. Antioxidant activity was evaluated by DPPH assay and anti-inflammatory studies were performed by Cox2 inhibition assay on THP1 cell lines. The results showed that MFAP9 was cytotoxic against A549 cells with an IC50 value of 29 mu g/mL and exhibited apoptotic properties after treatment with a concentration of 50 mu g/mL. In addition, MFAP9 showed higher DPPH radical scavenging activity than standard antioxidants at the same concentration and significant anti-inflammatory activity. The rate of MFAP9 production was optimized by a time course experiment. Characterization revealed that the maximum active temperature of MFAP9 is 50 degrees C, that it is stable at basic pH, and that 1 mM concentrations of Ni2+, Cd2+, and Mg2+ promote peptide activity. It is hoped that MFAP9 could become a promising new therapeutic agent for various clinical applications by using advances in proteomics, bioinformatics and modification strategies.

Key words

Antibacterial peptide/Anti-proliferative/Aspergillus fumigatus/MFAP9/ANTIFUNGAL PROTEIN/BIOACTIVE PEPTIDES/MARINE/PURIFICATION/GROWTH/ACID/L.

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出版年

2022
International journal of peptide research and therapeutics.

International journal of peptide research and therapeutics.

ISSN：1573-3149
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