首页|Development of M cell-targeting starch-based nanomicelles for oral delivery of immunoactive peptides
Development of M cell-targeting starch-based nanomicelles for oral delivery of immunoactive peptides
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NSTL
Elsevier
? 2022 Elsevier B.V.We prepared nine residue peptide CKSTHPLSC (CKS9)-grafted starch stearate for the simultaneous realization of tyrosinase related protein-2 (Trp-2) shielding and microfold cell (M cell) targeting. The suited introduction of CKS9 and stearic acid endowed the prepared nanomicelles (200 ~ 250 nm) with the lower critical micelle concentration (0.0209 mg/mL) and affinity constant (2.10 ×10?7), robust encapsulation efficiency (61.59 ~ 72.23%) and loading capacity (49.43 ~ 55.71%), controllable release of Trp-2 peptide, excellent structure stability against the changes of physiological conditions, ultra-dilution and long-term storage. Approx.72% of the loaded cargos went through apical-to-basolateral transportation. The M cell targeting affinity of the designed micelles is 1.5 times stronger than that towards Caco-2 cells. All these performances can be ascribed to the special framework of the designed polymer, namely, the hydrophobic stearic acid side chains and stable M cell-targeting ligand with a higher proportion of hydrophilic domain, which ensured the formation of steady core-shell spherical nanomicelles and the full exposure of targeting ligand. These rationally designed micelles provide a promising potential for developing stable and effective drug delivery systems applicable for oral immunization.
Ministry of Education Engineering Research Center of Starch and Protein Processing Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety School of Food Science and Engineering South China University of Technology
Experimental Education/Administration Center National Demonstration Center for Experimental Education of Basic Medical Sciences School of Basic Medical Sciences Southern Medical University
Henan Engineering Laboratory for Bioconversion Technology of Functional Microbes College of Life Science Henan Normal University
NSTL
Elsevier
