Phytochemistry2022，Vol.1958.DOI:10.1016/j.phytochem.2021.113052

Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 Co-cultures using thermal desorption- Comprehensive two-dimensional gas chromatography- Time-of-flight mass spectrometry (TD-GCxGC-TOFMS)

Campos, O. Rene Arredondo Bannon, Catherine C. de la Mata, A. Paulina Case, Rebecca J. Harynuk, James J. Armstrong, Michael D. Sorochan
Phytochemistry2022，Vol.1958.DOI:10.1016/j.phytochem.2021.113052
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  • NSTL
  • Elsevier

Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 Co-cultures using thermal desorption- Comprehensive two-dimensional gas chromatography- Time-of-flight mass spectrometry (TD-GCxGC-TOFMS)

Campos, O. Rene Arredondo 1Bannon, Catherine C. 2de la Mata, A. Paulina 1Case, Rebecca J. 3Harynuk, James J. 1Armstrong, Michael D. Sorochan1
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作者信息

  • 1. Univ Alberta
  • 2. Dalhousie Univ
  • 3. Nanyang Technol Univ
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Abstract

Dunaliella tertiolecta is a marine microalgae that has been studied extensively as a potential carbon-neutral biofuel source (Tang et al., 2011). Microalgae oil contains high quantities of energy-rich fatty acids and lipids, but is not yet commercially viable as an alternative fuel. Carefully optimised growth conditions, and more recently, algal-bacterial co-cultures have been explored as a way of improving the yield of D. tertiolecta micro algae oils. The relationship between the host microalgae and bacterial co-cultures is currently poorly understood. Here, a complete workflow is proposed to analyse the global metabolomic profile of co-cultured D. tertiolectra and Phaeobacter italicus R11, which will enable researchers to explore the chemical nature of this relationship in more detail. To the best of the authors' knowledge this study is one of the first of its kind, in which a pipeline for an entirely untargeted analysis of the algal metabolome is proposed using a practical sample preparation, introduction, and data analysis routine.

Key words

Dunaliella tertiolecta/Dunaliellaceae/Phaeobacter italicus/Rhodobacteraceae/Biofuels/Machine learning/Metabolomics/GCxGC/MICROALGAE OIL/EXTRACTION/BACTERIA

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出版年

2022
Phytochemistry

Phytochemistry

CCR
ISSN：0031-9422
被引量3
参考文献量31
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