Synthesis of Nicotinamide Mononucleotide from Xylose via Coupling Engineered Escherichia coli and a Biocatalytic Cascade

Ngivprom, Utumporn ¹Lasin, Praphapan ¹Khunnonkwao, Panwana ¹Worakaensai, Suphanida ¹Jantama, Kaemwich ¹Kamkaew, Anyanee ¹Lai, Rung-Yi¹

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作者信息

1. Suranaree Univ Technol
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Abstract

beta-Nicotinamide mononucleotide (NMN) has recently gained attention for a nutritional supplement because it is an intermediate in the biosynthesis of nicotinamide adenine dinucleotide (NAD(+)). In this study, we developed NMN synthesis by coupling two modules. The first module is to culture E. coli MG1655 tktA tktB ptsG to metabolize xylose to generate D-ribose in the medium. The supernatant containing D-ribose was applied in the second module which is composed of EcRbsK-EcPRPS-CpNAMPT reaction to synthesize NMN, that requires additional enzymes of CHU0107 and EcPPase to remove feedback inhibitors ADP and pyrophosphate. The second module can be rapidly optimized by comparing NMN production determined by the cyanide assay. Finally, 10 mL optimal biocascade reaction generated NMN with a good yield of 84 % from 1 mM D-ribose supplied from the supernatant of E. coli MG1655 tktA tktB ptsG. Our results can further guide researchers to metabolically engineer E. coli for NMN synthesis.

Key words

biocatalytic cascade/E/coli/nicotinamide mononucleotide/ribose/xylose/NUCLEOTIDE ANALOGS/RIBOKINASE/BIOSYNTHESIS/NUCLEOSIDE/PHOSPHATE/RIBOSIDE/PATHWAY/BINDING/CANCER

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出版年

2022

Chembiochem

CCR

ISSN：1439-4227

被引量9

参考文献量46

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