首页|Biotechnological production of D-tagatose from lactose using metabolically engineering Lactiplantibacillus plantarum
Biotechnological production of D-tagatose from lactose using metabolically engineering Lactiplantibacillus plantarum
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NSTL
Elsevier
D-Tagatose is a low-calorie sweetener with health benefits. To produce D-tagatose from lactose, a metabolic engineering Lactiplantibacillus plantarum was constructed. The galactokinase gene galK was inactivated and the original promoter of beta-galactosidase gene lacLM was replaced by a strong lactose/galactose-inducible promoter PlacLM-35-10 in Lpb. plantarum WCFS1. Subsequently, the L-arabinose isomerase gene AraA derived from Lactobacillus casei SDMCC050286 was over-expressed. A recombinant strain with the potential of D-tagatose production was obtained. Its galactose metabolism pathway was blocked, and beta-galactosidase activity was increased by 5.4-folds. Meanwhile, the heterogeneous L-arabinose isomerase was highly expressed in this strain. The optimal conditions for D-tagatose production by resting cells of the recombinant strain were at 65 degrees C and pH 7.5. Furthermore, the conversion rate of D-tagatose from 175 g/L lactose reached 33% at 56 h. This study verified the feasibility of one-pot production of D-tagatose from lactose by engineering strain of Lpb. plantarum, thereby laying down the foundation for industrial usage of lactose.
LactoseD-tagatoseWhole-cell catalysisMetabolic engineeringLactiplantibacillus plantarum
NSTL
Elsevier
