首页|Modification of the second PEP4-allele enhances citric acid stress tolerance during cultivation of an industrial rice wine yeast strain with one PEP4-allele disrupted

Modification of the second PEP4-allele enhances citric acid stress tolerance during cultivation of an industrial rice wine yeast strain with one PEP4-allele disrupted

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  • NSTL
  • Elsevier
To investigate the effect of PEP4 alleles on cellular tolerance of rice wine Saccharomyces cerevisiae against citric acid stress, one PEP4-allele disrupted strain and one PEP4-allele modified along with one PEP4-allele disrupted rice wine Saccharomyces cerevisiae strains were constructed. The PEP4-allele disruption and modification significantly decreased intracellular PrA activity, and a dose-response relationship was found between intracellular Proteinase A activity and number of intact PEP4-alleles. Under cultivation conditions whether with sufficiency or starvation of amino acid nitrogen source, the second PEP4-allele modification was all found beneficial for cellular citric acid stress tolerance of the industrial Saccharomyces cerevisiae strain SWY85. These results allude to PEP4-allele having a physiological role in the metabolic regulations correlated to citric acid tolerance in yeast cell.

Saccharomyces cerevisiaeSecond PEP4-Allele modificationProteinase ACitric acid stressCellular tolerance

Zhang, Hongbo、Shao, Feifei、Cong, Jianhang、Huang, Yan、Chen, Minfang、He, Wenxia、Zhang, Tao、Liu, Luyi、Yao, Mengzhou、Gwabin, Hannatu、Lin, Yingjiang

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Shaoxing Univ, Coll Life Sci, 900 Chengnandadao Rd, Shaoxing 312000, Peoples R China

2021

10.1016/j.lwt.2021.112286

LWT-Food Science & Technology

LWT-Food Science & Technology

ISSN：0023-6438
年,卷(期)：2021.152
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