首页|Serum amyloid protein (SAA) as a healthy marker for immune function in Tridacna crocea
Serum amyloid protein (SAA) as a healthy marker for immune function in Tridacna crocea
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NSTL
Elsevier
? 2022 Elsevier LtdSerum amyloid protein (SAA) is known as an acute reactive protein of innate immunity in mammals. However, in invertebrates, the role of SAA in innate immunity is still unclear. In this study, a full-length cDNA of the SAA gene (named TcSAA) was cloned from Tridacna crocea, mollusca. The gene includes a 193 bp 5′ untranslated region (UTR) and a 129 bp 3’ UTR sequence, and the open reading frame (ORF) with 393 bp nucleotides encodes a polypeptide of 130 amino acids. TcSAA contains a typical signal peptide and an SAA functional domain. The mRNA expression of TcSAA was detected in all 12 selected tissues and 7 different developmental stages. Furthermore, the expression of TcSAA was increased quickly in hemocytes after challenge with V. coralliilyticus or LPS. Furthermore, rTcSAA could bind V. coralliilyticus and V. alginolyticus, and the protein could reduce the lethality rate of the clams from 80% to 55% which caused by V. coralliilyticus about 48 h after injection. In summary, these results indicate that TcSAA may act as a marker for monitoring health and protecting T. crocea.
wang J.、Zhang J.、Yang Y.、Zhang A.、Zeng L.、Xiao S.、Ma H.、Li J.、Mao F.、Zhang Y.、Yu Z.、Xiang Z.
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State Key Laboratory of Developmental Biology of Freshwater Fish College of Life Sciences Hunan Normal University
Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine Biology South China Sea Institute of Oceanology Chinese Academy of Science
NSTL
Elsevier
