首页|Isolation and characterization of microsatellite loci from Quercus mongolica var. crispula
Isolation and characterization of microsatellite loci from Quercus mongolica var. crispula
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Microsatellites were isolated and characterized for the Japanese oak species, Quercus mongolica var. crispula, distributed in temperate deciduous forests of Japan. Eleven of the 48 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 67 Q. mongolica var. crispula individuals within a plot in southwestern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.522 to 0.896 and from 0.536 to 0.882, respectively. These polymorphic microsatellite markers are useful for estimating pollen-mediated gene flow in Q. mongolica var. crispula.
enrichmentmicrosatellitesQuercus mongolica var. crispulaDNA
Mishima K、Watanabe A、Isoda K、Ubukata M、Takata K
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Forest Tree Breeding Ctr, Hitachi, Ibaraki 3191301, Japan
Akita Prefectural Univ, Inst Wood Technol, Akita 0160876, Japan
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