首页|IN VITRO SEED GERMINATION AND SEEDLING DEVELOPMENT IN AERIDES ODORATA LOUR.
IN VITRO SEED GERMINATION AND SEEDLING DEVELOPMENT IN AERIDES ODORATA LOUR.
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In the present investigation, in vitro seed germination and seedling development of Aerides odorata Lour, was assessed on Murashige and Skoog, 1962 (MS) static media solidified with agar (0,8%) and supplemented with or without different concentrationand combination of a-Napthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). Hormone free MS medium was found to be best for early germination, higher number of protocorms formation and their further differentiation of A. odorata. However all the hormone treatments favored seed germination and the further differentiation into 2-3 leaved seedlings. Use of combination of BAP (2.0 mgl'1) and NAA (1.0 mgh1) proved to be the best for seed germination among all other hormone treatments. For the seed germination BAP was more effective than NAA and lower concentration of BAP and NAA were more effective than their respective higher concentration.
Bijaya Pant、Rajdeep Gurung
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Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal
NSTL
