首页|Comparative analysis on the immunoregulatory roles of ferritin M in hybrid fish (Carassius cuvieri female x Carassius auratus red var male) and its parental species after bacterial infection
Comparative analysis on the immunoregulatory roles of ferritin M in hybrid fish (Carassius cuvieri female x Carassius auratus red var male) and its parental species after bacterial infection
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NSTL
Elsevier
Ferritin M is involved in the regulation of fish immunity. In this study, open reading frame (ORF) sequences of ferritin M from hybrid fish and its parental species were 534 bp. Tissue-specific analysis indicated that the highest level of ferritin M from red crucian carp was observed in kidney, while peaked expressions of ferritin M from white crucian carp and hybrid carp were observed in gill. Elevated levels of ferritin M from hybrid carp and its parental species were detected in immune-related tissues following Aeromonas hydrophila infection or in cultured fish cell lines after lipopolysaccharide (LPS) challenge. Ferritin M overexpression could attenuate NF-kappa B and TNF alpha promoter activity in their respective fish cells. Purified ferritin M fusion proteins elicited in vitro binding activity to A. hydrophila and Edwardsiella tarda, lowered bacterial dissemination to tissues and alleviated inflammatory response. Furthermore, treatment with ferritin M fusion proteins could mitigate bacteria-induced liver damage and rescue antioxidant activity. These results suggested that ferritin M in hybrid fish showed a similar immune defense against bacteria infection in comparison with those of its parental species.
NSTL
Elsevier
