首页|Micronucleus formation during early cleavage division is a potential hallmark of preimplantation embryonic loss in cattle
Micronucleus formation during early cleavage division is a potential hallmark of preimplantation embryonic loss in cattle
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NSTL
In assisted reproductive technology (ART)-derived embryos of non-rodent mammals， including humans and cattle， chromosome segregation errors are highly likely to occur during early cleavage division， resulting in aneuploidy， including mosaicism。 However， the relationship between chromosomal segregation errors during early cleavage and subsequent embryonic development has not been detailed in these mammals。 In the present study， we developed non-invasive live-cell imaging of chromosome segregation dynamics using a histone H2B-mCherry mRNA probe in bovine preimplantation embryos。 Chromosome segregation errors in early cleavage affected blastocyst formation。 Especially， embryos that underwent abnormal chromosome segregation (ACS) with multiple or large micronucleus formation rarely developed into blastocysts。 Embryos with the severe ACS had prolonged cell cycle duration。 After transfer of blastocysts with live-cell imaging of chromosome segregation to ten cows， six became pregnant and four of them gave full-term offspring。 Interestingly， two of them were derived from blastocysts with ACS。 Hence， chromosomal segregation errors with micronucleus formation during early cleavage can be a fatal hallmark of preimplantation embryogenesis in cattle。 This technique has shown potential for understanding the relationship between chromosome segregation error and subsequent embryo development， and for selecting viable ART-derived embryos for medical and livestock production。
NSTL
