首页|Molecular mapping of <i>Arabidopsis thaliana</i> lipid-related orthologous genes in <i>Brassica napus</i>.

Molecular mapping of <i>Arabidopsis thaliana</i> lipid-related orthologous genes in <i>Brassica napus</i>.

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  • Springer Nature
Quantitative Trait Loci (QTL) for oil content has been previously analyzed in a SG-DH population from a cross between a Chinese cultivar and a European cultivar of <i>Brassica napus</i>. Eight QTL with additive and epistatic effects, and with environmental interactions were evaluated. Here we present an integrated linkage map of this population predominantly based on informative markers derived from <i>Brassica</i> sequences, including 249 orthologous <i>A. thaliana</i> genes, where nearly half (112) are acyl lipid metabolism related genes. Comparative genomic analysis between <i>B. napus</i> and <i>A. thaliana</i> revealed 33 colinearity regions. Each of the conserved <i>A. thaliana</i> segments is present two to six times in the <i>B. napus</i> genome. Approximately half of the mapped lipid-related orthologous gene loci (76/137) were assigned in these conserved colinearity regions. QTL analysis for seed oil content was performed using the new map and phenotypic data from 11 different field trials. Nine significant QTL were identified on linkage groups A1, A5, A7, A9, C2, C3, C6 and C8, together explaining 57.79% of the total phenotypic variation. A total of 14 lipid related candidate gene loci were located in the confidence intervals of six of these QTL, of which ten were assigned in the conserved colinearity regions and felled in the most frequently overlapped QTL intervals. The information obtained from this study demonstrates the potential role of the suggested candidate genes in rapeseed kernel oil accumulation.Digital Object Identifier http://dx.doi.org/10.1007/s00122-011-1716-3

crossescultivarsepistasisfield experimentationfield testsgene interactiongene mappinggenesgenetic mappinggeneticsgenomesinteractionslinkagelocimetabolismmolecular geneticsoilsphenotypesphenotypic variationplant oilsquantitative trait lociquantitative traitsraperapeseedseed oilsseedsArabidopsisArabidopsis thalianaBrassicaBrassica napusBrassica napus var. oleiferaBrassicaceaeCapparidalesdicotyledonsangiospermsSpermatophytaplantseukaryotesArabidopsisBrassicaBrassica napusbiochemical geneticscanolaCapparalescultivated varietieshypostasisoilseed rapephenotypic variabilityvegetable oils

Wang Hao、Xu AiXia、Huang RuiZhi、Li DianRong、Meng JinLing、Ni XiYuan、Xu HaiMing、Wang YiLong、Jiang ChonChon、Huang JiXiang、Chen Fei、Zhao JianYi、Xu Fei

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College of Agricultural and Biotechnology, Zhejiang University, Hangzhou, China

College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, China

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China

Hybrid Rapeseed Research Center of Shaanxi, Dali, China

College of Agronomy, Northwest Agricultural and forestry University, Yanglin, China

Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou, China

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2012

10.1007/s00122-011-1716-3

Theoretical and Applied Genetics

Theoretical and Applied Genetics

EISCI
ISSN：0040-5752
年,卷(期)：2012.124(2)
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