Efficient synthesis of terminal-diazirine-based histone peptide probes
Fan, Jian 1Shu, Qingyao 1Li, Yi-Ming 2Shi, Jing1
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作者信息
1. Univ Sci & Technol China
2. Hefei Univ Technol
折叠
Abstract
Histone post-translational modifications (hPTMs) regulate series fundamental cellular processes through the specific recognition of "reader" or "effector" proteins. The chemical probes containing photo-cross -linking groups, especially diazirine group has been developed to capture proteins recognized by hPTMs. However, the construction of diazirine-based photo-reactive amino acids requires multiple syn-thetic steps. Herein, we report the efficient synthesis of a new diazirine-based histone peptide probes through the construction of terminal diazirine via phenyliodonium diacetate (PIDA) mediated transfor-mation. The key building block tert-butyl 3-(3H-diazirin-3-yl)propanoate can be obtained in one step with 90% yield. The synthetic H3K4Me3 photo-cross-linking probe was demonstrated to label H3K4Me3 specific reader in vitro and in cell lysates. (c) 2022 Published by Elsevier Ltd.
Key words
Histone post -translational modifications/Diazirine-based probes/Photo-crosslinking/Specific reader/CHEMICAL-SYNTHESIS/LIGATION/LYSINE-4/PROTEINS/H3/METHYLATION/UBIQUITIN/READERS
NSTL
Elsevier