首页|The role of ATP in solubilizing RNA‐binding protein fused in sarcoma
The role of ATP in solubilizing RNA‐binding protein fused in sarcoma
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NSTL
Wiley
Abstract Intrinsically disordered protein (IDP) plays an important role in liquid–liquid phase separation (LLPS). RNA‐binding protein fused in sarcoma (FUS) is a well‐studied IDP that induces LLPS since its low‐complexity core region (FUS‐LC‐core) is essential for droplet formation through contacts between FUS‐LC‐cores. Several experimental studies have reported that adenosine triphosphate (ATP) concentrations modulate LLPS‐driven droplet formation through the dissolution of FUS. To elucidate the role of ATP in this dissolution, microsecond‐order all‐atom molecular dynamics (MD) simulations were performed for a crowded system of FUS‐LC‐cores in the presence of multiple ATP molecules. Our analysis revealed that the adenine group of ATP frequently contacted the FUS‐LC‐core, and the phosphoric acid group of ATP was exposed to the external solvent, which promoted both hydration and solubilization of FUS.
adenosine triphosphate crowdingdissolutionintrinsically disordered proteinRNA‐binding protein fused in sarcomasolubilization
Hayato Aida、Yasuteru Shigeta、Ryuhei Harada
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College of Biological Sciences,University of Tsukuba
Center for Computational Sciences,University of Tsukuba
NSTL
Wiley
