首页|Long-term cell suspension culture of Thymus persicus (Lamiaceae): A novel approach for the production of anti-cancer triterpenic acids

Long-term cell suspension culture of Thymus persicus (Lamiaceae): A novel approach for the production of anti-cancer triterpenic acids

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  • NSTL
  • Elsevier
Triterpenic acids (TAs) such as betulinic acid (BA), oleanolic acid (OA), and ursolic acid (UA) are well-known natural compounds found in many plant taxa possessive a wide spectrum of biological activities and potential medicinal uses. Thymus persicus has been already reported as a rich source of TAs. Nowadays, long-term culture is an efficient technique in increasing biosynthesis and production of specialized metabolites in the plant cell suspension cultures with the possibility of stimulation, metabolic regulation, permeabilization, and up-scaling to bioreactors. In the present study, both batch and long-term cell suspension culture (CSC) of the plant were developed for the production of these compounds. A maximum specific cell growth rate of 5.7 g day(-1), a doubling time of 42 h, and the highest cell viability (94.7%) throughout the growth period were measured. The highest cell fresh weight in CSC and its long-term culture was 100.63 +/- 4.2 and 171.6 +/- 4.1 g L-1 at day 21 and 35 of culture, respectively. A positive correlation between cell growth and the content of TAs in long-term culture was observed. The plant cells produced the highest levels of TAs in the mid-exponential phase and held their production stability during the long-term culture period. The highest content (mg 100 g(-1) DW) of BA (163.11 +/- 4.3) was measured in the second week of culture, while the maximum level of OA (10.22 +/- 0.1) and UA (11.01 +/- 0.4) was recorded in the third week of culture period. The present findings can be interestingly considered for further commercial production of valuable TAs at large-scale cultures in bioreactors.

CallusCell viabilityLamiaceaeSpecialized metaboliteThymeTriterpenoidsPLANT-GROWTH REGULATORSVITRO CALLUS INDUCTIONURSOLIC ACIDPENTACYCLIC TRITERPENOIDSSATUREJA-KHUZISTANICASECONDARY METABOLITESROSMARINIC ACIDOLEANOLIC ACIDBETULINIC ACIDBIOACTIVE TRITERPENES

Bakhtiar, Ziba、Mirjalili, Mohammad Hossein

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Shahid Beheshti Univ

2022

10.1016/j.indcrop.2022.114818

Industrial Crops and Products

Industrial Crops and Products

EISCI
ISSN：0926-6690
年,卷(期)：2022.181
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