首页|Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection

Dynamics of Reverse Transcription-Polymerase Chain Reaction and Serologic Test Results in Children with SARS-CoV-2 Infection

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  • NSTL
  • Elsevier
Objectives To determine the time to reverse transcription-polymerase chain reaction (RT-PCR) negativity after the first positive RT-PCR test, factors associated with longer time to RT-PCR negativity, proportion of children seroconverting after proven severe acute respiratory syndrome coronavirus 2 infection, and factors associated with the lack of seroconversion. Study design The Epidemiological Study of Coronavirus in Children of the Spanish Society of Pediatrics is a multicenter study conducted in Spanish children to assess the characteristics of coronavirus disease 2019. In a subset of patients, 3 serial RT-PCR tests on nasopharyngeal swab specimens were performed after the first RTPCR test, and immunoglobulin G serology for severe acute respiratory syndrome coronavirus 2 antibodies was performed in the acute and follow-up (<14 and >= 14 days after diagnosis) phase. Results In total, 324 patients were included in the study. The median time to RT-PCR negativity was 17 days (IQR, 8-29 days), and 35% of patients remained positive more than 4 weeks after the first RT-PCR test. The probability of RT-PCR negativity did not differ across groups defined by sex, disease severity, immunosuppressive drugs, or clinical phenotype. Globally, 24% of children failed to seroconvert after infection. Seroconversion was associated with hospitalization, persistence of RT-PCR positivity, and days of fever. Conclusions Time to RT-PCR negativity was long, regardless of the severity of symptoms or other patient features. This finding should be considered when interpreting RT-PCR results in a child with symptoms, especially those with mild symptoms. Seroprevalence and postimmunization studies should consider that 11 in 4 infected children fail to seroconvert.

COVID-19

Tagarro, Alfredo、Jose Sanz-Santaeufemia, Francisco、Grasa, Carlos、Cobos, Elena、Yebra, Julia、Antonio Alonso-Cadenas, Jose、Baquero-Artigao, Fernando、Miguel Mesa-Guzman, Juan、Perez-Seoane, Beatriz、Calvo, Cristina、Luisa Herreros, Maria、Epalza, Cristina、Melendo, Susana、Dominguez-Rodriguez, Sara、Vidal, Paula、Pacheco, Monica、Ballesteros, Alvaro、Bernardino, Maria、Villanueva-Medina, Sara、Rodriguez-Molino, Paula、Miragaya Castro, Sandra、Riviere, Jacques、Garces, Rosa、Santiago, Begona、Fumado, Victoria、Urretavizcaya-Martinez, Maria、Luz Garcia-Garcia, Maria、Penin, Maria、Cava, Fernando、Saez, Elena、Isabel Iglesias-Bouzas, Maria、Herrero, Blanca、de Jesus Reinoso, Teresa、Moraleda, Cinta、Group, EPICO-AEP Working

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Pediat Dept,Hosp Univ Infanta Sofia

Pediat Dept,Hosp Infantil Univ Nino Jesus

Inst Invest Hosp La Paz IDIPaz,Hosp Univ La Paz

Inst Invest 12 Octubre Imas12,Fdn Invest Biomed Hosp 12 Octubre

Emergency Dept,Hosp Infantil Univ Nino Jesus

Dept Pediat,Hosp Univ Vail dHebron

Dept Pediat Dept,Hosp Clin Univ Lozano Blesa Zaragoza

Pediat Res Grp,Europea Univ Madrid

Pediat Dept,Hosp Univ 12 Octubre

Pediat Infect Dis Unit,Hosp Univ Gregorio Maranon

Pediat Infect Dis Unit,Hosp Univ St Joan Deu Barcelona

Pediat Dept,Complejo Hosp Navarra

Pediat Dept,Hosp Univ Severo Ochoa Madrid

Pediat Dept,Hosp Univ Principe Asturias

Microbiol Dept,Hosp Univ Infanta Sofia

Pediat Intens Care Unit,Hosp Infantil Univ Nino Jesus

Oncol Dept,Hosp Infantil Univ Nino Jesus

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2022

10.1016/j.jpeds.2021.09.020

The Journal of pediatrics

The Journal of pediatrics

ISSN：0022-3476
年,卷(期)：2022.241
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