首页|Determination of isoscoparin in mouse blood using UPLC–MS/MS and its pharmacokinetics
Determination of isoscoparin in mouse blood using UPLC–MS/MS and its pharmacokinetics
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NSTL
Abstract This study developed a UPLC–MS/MS method to detect isoscoparin in mouse blood, determined the pharmacokinetics of isoscoparin in mice after intravenous (5?mg/kg) and intragastric (20?mg/kg) administration, and calculated the absolute bioavailability. A HSS T3 column was used for separation, and the column temperature was set at 40°C. The mobile phases were acetonitrile and 0.1% formic acid, and the gradient elution procedure was used. The blood sample was treated with protein precipitant with acetonitrile–methanol (9:1, v/v). Multiple reaction monitoring mode was used for quantitative analysis in electrospray positive‐ion mode. It showed a good linear relationship in the range of 1–4000?ng/mL (r?>?0.998); the intra‐day and inter‐day precision was <12%, and the accuracy was 86–112%. The recovery was >68%, and the matrix effect was 86–90%. The half‐life of isoscoparin was relatively short in mice, and the bioavailability was 2.6%. The developed UPLC–MS/MS method was rapid, sensitive, and suitable for the pharmacokinetics of isoscoparin in mice.
NSTL
