首页|Bioactive Zinc(II) complex incorporated PCL/gelatin electrospun nanofiber enhanced bone tissue regeneration

Bioactive Zinc(II) complex incorporated PCL/gelatin electrospun nanofiber enhanced bone tissue regeneration

扫码查看
原文链接
  • NSTL
  • Elsevier
Bone tissue regeneration is augmented by biocompatible nanofiber scaffolds, that supports reliable and enhanced bone formation. Zinc is an essential mineral that is vital for routine skeletal growth and it emerges to be able to improve bone regeneration. Phytochemicals, particularly flavonoids have achieved prominent interest for their therapeutic ability, they have demonstrated promising effects on bone by encouraging osteoblastogenesis, which finally leads to bone formation. In this study, we have synthesized bioactive zinc(II) quercetin complex material and used for nanofibers scaffold fabrication to enhance bone tissue regeneration property. Two derivatives of zinc(II) quercetin complexes [(Zn(quercetin) (H2O)(2)) (Zn+Q), and Zn(quercetin)(phenanthroline) (Zn+Q(PHt)) have been synthesized and characterized using UV-Visible spectrophotometer and Fourier Transform-IR spectroscopy. The UV-Visible absorption and IR spectra prove the B-ring chelation of the flavonoid quercetin to zinc (II) rather C-ring chelation. The potential ability of the above synthesized metal complexes on osteogenesis and angiogenesis have been studied. Besides the bioactivity of the metal complexes, the control quercetin has also been examined. The chick embryo chorioallantoic membrane (CAM) assay demonstrated that the angiogenic parameters were increased by the (Zn+Q(PHt)) complex. Amongst, (Zn+Q(PHt)) complex showed significant activity and thereby this complex has been further examined for the bone tissue activity by incorporating the complex into a nanofiber through electrospinning method. At the molecular level, Runx2, mRNA and protein, ALP and type 1 collagen mRNAs, and osteoblast-specific microRNA, pre-mir-15b were examined using real time RT-PCR and Western blot assay. Histology studies showed that the (PCL/gelatin/Zn+Q(PHt)) was biocompatibility in-ovo. Overall, the present study showed that quercetin-zinc complex (Zn+Q(PHt)) incorporated into PCL/gelatin nanofiber can act as a pharmacological agent for treating bone associated defects and promote bone regeneration.

Bioactive Zn complexBone tissue engineeringElectrospun nanofiberPCL/gelatinOsteogenesisALP activityMetal complex

Preeth, Desingh Raj、Saravanan, Sekaran、Shairam, Manickaraj、Selvakumar, Narasimman、Raja, Iruthayapandi Selestin、Dhanasekaran, Anuradha、Vimalraj, Selvaraj、Rajalakshmi, Subramaniyam

展开 >

Anna Univ, Chem Biol & Nanobiotechnol Lab, AU KBC Res Ctr, MIT, Chennai 600044, Tamil Nadu, India

SASTRA Univ, Ctr Nanotechnol & Adv Biomat CeNTAB, Sch Chem & Biotechnol, Dept Bioengn, Thanjavur

Anna Univ, Ctr Environm Studies, Chennai 600025, Tamil Nadu, India

Pusan Natl Univ, BIO IT Foundry Technol Inst, Busan, South Korea

Anna Univ, Ctr Biotechnol, Chennai 600025, Tamil Nadu, India

展开 >

2021

10.1016/j.ejps.2021.105768

European journal of pharmaceutical sciences

European journal of pharmaceutical sciences

ISTP
ISSN：0928-0987
年,卷(期)：2021.160
  • 20
  • 52