首页|Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance
Highly effective Candida rugosa lipase immobilization on renewable carriers: Integrated drying and immobilization process to improve enzyme performance
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NSTL
The overall stability of Candida rugosa lipase(CRL)was improved via immobilization onto low-cost eco-friendly carriers(agroindustrial wastes)followed by using spouted bed dryer or spray dryer.Lipase covalently immobilized on glutaraldehyde-activated rice husk proved the greatest performance,retaining 94.1% of the initial activity,followed by sugarcane bagasse(90.3%)and green coconut fiber(89.3%).On the other hand,the enzyme activity retention of CRL-lipase bioencapsulated in magnetic chitosan treated with glutaraldehyde or sodium tripolyphosphate ranged from 73.6% to 84.6%.The dried product moisture content,and water activities ranged from 4.1% and 6.5% and from _(0.2)to 0.35,respectively.After storage for six months at 5 ℃,the immobilized enzyme systems retained at least 70.0% of its initial activity(vs 18.3% retained activity for the free lipase).After ten reuse cycles,enzyme immobilized onto lignocellulosic carriers retained on average 72.7% of its initial activity.The thermostability of all immobilized derivatives was significantly improved and lipase immobilized onto rice husk showed a stabilization factor of 2_(0.4)4 at 80 ℃.The kinetic data showed that the CRL-lipase suffered alterations in catalytic behavior after the immobilization/drying,mainly a slight decrease in affinity to the substrate(↑KM).Among all the assessed immobilized lipase for aroma synthesis the rice husk immobilized lipase displayed the best result towards the production of isoamyl caprylate(62.40 g.L~(-1)).In conclusion,this report reinforces the urgent need for the economic development of eco-sustainable immobilized biocatalysts to boost the use of enzymatic technology on industrial scale,and the feasibility of the association of immobilization and drying processes to improve the enzyme-quality features.
Spray dryerSpouted bed dryerRenewable carriersEnzyme immobilizationAroma production
T.A.Costa-Silva、A.K.F.Carvalho、C.R.F.Souza
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Faculty of Pharmaceutical Sciences of Ribeirao Preto,Uniuersity of Sao Paulo,Brazil
Engineering School of Lorena,University of Sao Paulo,Brazil
NSTL
