首页|iversification of flavonoid accumulation among ecotypes of Agriophyllum squarrosum (L.) Moq. in response to drought stress
iversification of flavonoid accumulation among ecotypes of Agriophyllum squarrosum (L.) Moq. in response to drought stress
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中国科学院科技论文预发布平台
griophyllum squarrosum (L.) Moq., commonly known as sandrice, is an annual medicinal plant prevalent in the dunes across China's deserts. A garden trial revealed that flavonoid content varies among sandrice ecotypes due to long-term local adaptation to water variability. To investigate how sandrice responds to drought stress through the molecular metabolic regulation of flavonoids, we employed transcriptomic and metabolomic analyses during a 9-d ambient drought stress, examining three ecotypes along a precipitation gradient. The three ecotypes located in Dengkou (DK) County, Dulan (DL) County, and Aerxiang (AEX) village of northern China, which had 137, 263, and 485 mm precipitation, respectively. Soil moisture content was 4.04% after drought stress, causing seedlings of the three sandrice ecotypes to display collapsed structures, yellowing leaves, wilting, and curling. Among these, DL exhibited superior drought tolerance, in which plant height increase (PHI) and leaf area (LA) were significantly higher than those of DK and AEX. Flavonoid-targeted metabolomics identified that rutin, isoquercitrin, and astragalin constituted over 95.00% of the 15 flavonoid metabolites detected. A total of 12 differentially accumulated flavonoids (DAFs) were found, with rutin being the most abundant (1231.572859.34 ng/100 mg fresh weight (FW)), showing a gradual increase along the precipitation gradient. Transcriptomic analysis revealed 14 common differentially expressed genes (DEGs) associated with flavonoid synthesis among the three ecotypes. Integrative analysis of DEGs and DAFs indicated that sandrice adapts to drought stress by activating different flavonoid synthesis pathways. In DK, the dihydrokaempferol-dihydroquercetin pathway, regulated by flavonoid 3'-monooxygenase (CYP75B1), likely enhances drought adaptation. In AEX, transcriptional repression by O-methylatransferase (OMT) shifts the metabolic flux from the quercetin-isorhamnetin pathway to the quercetin-isoquercetin-rutin pathway in response to drought. DL, the most drought- tolerant ecotype, appears to activate the naringenin-apigenin-luteolin route and employs a unique flavonoid accumulation pattern in response to drought stress. Our data reveal that flavonoid synthesis in sandrice is fine-tuned among ecotypes to cope with drought, offering valuable germplasm resources and evaluation methods for sandrice acclimation and providing insights into drought response in non-model plants.
中国科学院科技论文预发布平台
