BackgroundCancer patients often have symptoms such as muscle lossbut its pathological mechanism is not fully understood. ObjectiveTo investigate the pathological mechanism of tumor-related skeletal muscle injury and the intervention effect of Yiqi Chutan methodYQCT on tumor-related skeletal muscle injury. MethodsAn experiment was conducted from October 2020 to December 2020a total of thirty 8-week-old female C57BL/6 mice were used. Twenty mice were randomly selected and the tumor-bearing mouse model was established by subcutaneous injection of tumor cells. The model was divided into tumor groupn=10 and YQCT groupn=10and a control groupn=10 was set. The YQCT group was treated with ingastric administration of Yifei Sanjie Pills3 gkg-1d-1 control group and tumor group were treated with the same volume of normal saline for a total of 21 days. 24 hours after the last administrationbehavioral assessment was conducted using the open field test and the elevated cross maze test. The gastrocnemius muscle tissues were collected for histopathological analysis to observe the pathological damage changes of the gastrocnemius muscle tissues. Transcriptomic analysis was adopted to observe the differentially expressed genes. The levels of inflammatory factors in tissues were detected by enzyme-linked immunosorbent assay ELISA. RAW264.7 cells in the logarithmic growth phase were randomly divided into the mouse serum control group RAW264.7 groupthe mouse tumor serum group RAW264.7-LPS groupand the mouse qi-tonifying and phlegm-eliminating serum groupYQCT group. The RAW264.7 group was treated with the medium containing 10% serum from control groupthe RAW264.7-LPS group was treated with the medium containing 10% serum from tumor group mice +100 ng/mL LPSand YQCT group was treated with the medium containing 10% serum from YQCT group +100 g/L LPS for 24 hours. The levels of inflammatory factors in RAW264.7 were detected by ELISA. After the RAW264.7 cells treated above were co-cultured with mouse C2C12 cells for 48 hours, the autophagic lysosome levels of C2C12 cells were detected using a lysosome red fluorescent probe kit. ResultsThe comparison of the total movement distance in the field experiment and the total movement path in the elevated cross maze experiment among the three groups of mice showed statistically significant differencesP<0.001 Among them, the tumor group was lower than the control groupand the YQCT group was higher than the tumor groupP<0.001. The pathological results showed that compared with the control group the muscle cells in the tumor group were significantly damagedwhile the degree of damage in the YQCT group was reduced. The results of transcriptomic analysis showed that the gene expression trends in the control group and the YQCT group were relatively uniformwhile the gene expression trends in the tumor group were opposite. The results of immunofluorescence staining showed that compared with the control groupthe infiltration of M1 type macrophagesneutrophilsT lymphocytes and B lymphocytes in the gastrocnemius muscle of the tumor group increased. Compared with the tumor groupthe infiltration of M2 type macrophages in the YQCT group significantly increasedwhile the infiltration of other pro-inflammatory cells decreased. The ELISA results showed that the levels of IL-1IL-6 and TNF- in the tumor group were higher than those in the control group, while the levels of IL-1L-6 and TNF- in the YQCT group were lower than those in the tumor groupP<0.001. The results of the cell experiment showed that the levels of IL-1IL-6 and TNF- in the RAW264.7-LPS group were higher than those in the control groupand the levels of IL-1IL-6 and TNF- in the YQCT group were lower than those in the RAW264.7-LPS groupP<0.001. Moreoveractivated RAW264.7 cells can lead to an increase in autophagic lysosomes in C2C12 cells. ConclusionYQCT reduces the infiltration of skeletal muscle inflammatory cells caused by tumorthereby alleviating the inflammatory injury of skeletal muscle and ultimately protecting the motor function of tumor-bearing mice.
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