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Somatic Cell Selection to Genetically Improve Plant Water-Use Efficiency andTolerance to Stresses
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Water conservation may depend on development of improved crop plant water-useefficiency and tolerances to drought and heat stresses. This project tested the hypothesis that increased production of uncommon polyamines may confer greater crop water-use efficiency and/or tolerances to drought and heat stresses, using alfalfa (Medicago sativa L.) and cotton (Gossypium hirsutum L.) as model systems. The genetic strategy involved selection for cellular tolerance to known inhibitors of three biosynthetic enzymes in the polyamine pathway, which should lead to overproduction of the targeted polyamines; and evaluation of selected and nonselected cell lines for cellular tolerance to induced water deficit (alfalfa) or heat (cotton) stresses.
Water conservationFarm cropsPlant physiologyWater utilizationPlantgeneticsHeat stressDroughtsCotton plantsAlfalfaPlant ecologyPlant cellsPolyaminesPlant tissues
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