查看更多>>摘要：swelling usually initiates the regulatory volume decrease (RVD) process mediated mainly by volume-regulated anion channels (VRACs), which are formed by multiple different leucine-rich repeat containing protein 8 (LRRC8) family members. VRAC currents have been widely recorded in astrocytes, neurons and microglia in the brain, and VRACs have been suggested to be involved in the important pathogenesis of cell swelling-related central nervous system (CNS) diseases, such as ischemic stroke, epilepsy and epileptogenesis, glioblastoma (GBM), and so on. Recently, the increasing studies started to focus on LRRC8A (SWELL1), an obligatory subunit of VRAC indentified in 2014, which may be the key target to regulate the VRAC functions. After cerebral ischemia, the swollen astrocytes, neurons and microglia can activate LRRC8A-dependent VRACs, which may respectively promote the release of excitatory amino acids (EAA), interaction with ionotropic glutamate receptors, and regulating inflammation, suggesting the pleiotropic roles of LRRC8A in swollen brain cells. For the treatment of cell swelling-related CNS diseases, specific targeting LRRC8A may be a superior strategy to inhibit swollen-induced VRAC hyperactivity without blocking the normal VRAC function.(c) 2022 IBRO. Published by Elsevier Ltd. All rights reserved.
查看更多>>摘要：The authors regret that they detected an error in the merged image of Dextran/GFAP in FASN shRNA group in figure 3B, which was inadvertently misplaced during figure assembly. In original experiments, Dextran (Texas Red) and IgG (labelled with Alexa Fluor 647) were observed on the same sample, and the GFAP channel was set as grey, the IgG channel red, then GFAP was merged with Dextran or IgG separately by using the image software FV10-ASW 4.2 viewer (Olympus). Having checked carefully, the authors confirmed that the merged image of Dextran/ GFAP in FASN shRNA group in figure 3B was inadvertently misplaced during figure assembly, and now it was replaced by the correct one. In figure 6B, the GFAP channel in the two merged images in C75+ saline group came from the same sample and treated by the same image processing as forementioned, which might cause unnecessary misunderstanding. The authors also would like to improve it. The error does not affect the results and conclusions of the paper. The corrected figure 3 and improved figure 6 were shown as below. The authors would like to apologise for any inconvenience caused.
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