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Plant Physiology and Biochemistry
Gauthier-Villars
Plant Physiology and Biochemistry

Gauthier-Villars

0981-9428

Plant Physiology and Biochemistry/Journal Plant Physiology and BiochemistrySCIISTP
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    Transcriptome sequencing and flavonoid metabolism analysis in the leaves of three different cultivars of Acer truncatum

    Qiao, QianSi, FenfenWu, ChongWang, Jiangyong...
    13页
    查看更多>>摘要：Young and mature leaves of three Acer truncatum varieties with different leaf colors were examined. Transcriptome sequencing and flavonoid metabolism were used to analyze the differential gene expression associated with different leaf colors and growth stages and the relationships between gene expression and flavonoid and anthocyanin contents to improve ornamental value and develop flavonoid-rich A. truncatum. Kyoto Encyclopedia of Genes and Genomes database annotation of differentially expressed genes indicated that the following genes were related to flavonoid synthesis: phenylpropanoid biosynthesis genes (PAL, C4H, 4CL and CHS), flavonoid biosynthesis genes (E2.1.1.104, CHI, FLS, F3'5'& nbsp;H and ANR), anthocyanin biosynthesis genes (ANS, DFR, HCT, BZ1, GT1, and UGT79B1), isoflavonoid biosynthesis genes (HIDH and CYP81E17), and their transcriptional regulator (MYB). A total of 234 types of flavonoids were detected. The types and contents of anthocyanins in the red-leaf varieties 'Hong Jingling' and 'Caidie Fanfei' were significantly higher than those in the green leaf cultivar 'Lv Baoshi', especially morning glory 3-O-glucoside, delphinidin 3-O-glucoside, and pelargonium-3-Oglucoside, which were not detected in 'Lv Baoshi'. Combined omics analysis showed that downregulated expression of C4H, CHS and F3'5'H and upregulated expression of FLS reduced the supply of raw materials for anthocyanin synthesis, and downstream ANR upregulation converted anthocyanins to procyanidins, increasing the total flavonoid content. F3'5'H expression was downregulated in the leaves of each variety with development, resulting in the accumulation of catechins and the gradual greening of the leaves. F3 & PRIME;5 & PRIME;H was significantly depleted in the young leaves of 'Hong Jingling' and 'Caidie Fanfei' compared with the young leaves of 'Lv Baoshi', while ANS and BZ1 were enriched significantly. It is concluded that F3'5'H, BZ1, and ANS are the key genes needed for breeding red A. truncatum and that ANR is the key gene needed for breeding varieties with a high flavonoids contens. These results may facilitate genetic modification or selection for further improvement of the ornamental qualities and flavonoid content of A. truncatum.
      原文链接:
    • NSTL
    • Elsevier

    Spatio-temporal characterization of the fruit metabolism in contrasting accessions of Macauba (Acrocomia aculeata)

    dos Santos Sa, Ana CarolinaOmena-Garcia, Rebeca PatriciaPereira, Greice LealRodrigues-Salvador, Acacio...
    12页
    查看更多>>摘要：Although Macauba (Acrocomia aculeata) has been highlighted by its high-quality oil to fit edible and nonedible purposes, data addressing carbon and nitrogen metabolism underlying development and ripening of fruits remain scarce. In addition, accessions of Macauba exibit varied oil yield in fruits, including during the fruit development stages. Here, we monitored contents of carbohydrates, proteins, amino acids and lipids in the mesocarp and endosperm of Macauba fruits until ripening. We selected three accessions from different Brazilian regions (southeast, MG; northeast, PE; and central-west, MS) that differ in the mesocarp lipid content of ripe fruits. Despite the anatomical differences, mesocarp and endosperm exhibited similar trends of metabolite accumulation for most of the analyzed compounds. In the mesocarp, total soluble protein, free amino acids, sucrose, starch and total lipids accumulate towards ripening, while glucose and fructose declined in all accessions. Endosperm differed from mesocarp solely in the amino acid content, which decreased in ripe fruits. In the endosperm, accessions accumulated carbohydrates differently. Accession PE showed comparable fructose and starch contents in the endosperm between the beginning of fruit development and ripening, while in accessions MG and MS, both compounds decreased and increased, respectively, towards ripening. Accession MG was highlighted by its highest lipid content in the two tissues indicating its potential for energy and cosmetic industries. Our results provide novel insights into metabolic changes underlying development and ripening of Macauba fruits and variability in oil content among accessions, indicating new targets for breeding programs.
      原文链接:
    • NSTL
    • Elsevier

    Resveratrol exerts beneficial effects on the growth and metabolism of Lactuca sativa L

    Aranit, FabrizioIshii-Iwamoto, Emy LuizaAbenavoli, Maria RosaWagner, Ana Luiza Santos...
    12页
    查看更多>>摘要：In order to assist sustainable agriculture, new strategies and methods are being used based on the utilization of new natural molecules. These natural compounds can be used as potential natural crop protectors and growth promoters, and the elucidation of their modes/mechanisms of action can represent a big step towards cleaner agriculture free of agrochemicals. In the present paper, the mechanisms underlying the effects of exogenous resveratrol (R), a natural phytoalexin found in plants, on Lactuca sativa metabolism were investigated through physiological and metabolomic approaches. The results highlighted that R stimulates the growth of lettuce. A reduction of the O-2.-production in R-treated seedlings and an increase in the photosynthesis efficiency was observed, indicated by a higher Fv/Fm. The metabolomic analysis of lettuce seedlings treated with R identified 116 metabolites related to galactose, amino acids, sugar and nucleotide sugar, and ascorbate and aldarate metabolisms. Increased content of some polyamines and several metabolites was also observed, which may have contributed to scavenging free radicals and activating antioxidant enzymes, thus reducing oxidative damage and improving PSII protection in R-treated seedlings.
      原文链接:
    • NSTL
    • Elsevier

    ROS-scavenging-associated transcriptional and biochemical shifts during nectarine fruit development and ripening

    Vall-llaura, NuriaFernandez-Cancelo, PabloNativitas-Lima, IsabelEcheverria, Gemma...
    11页
    查看更多>>摘要：ROS are known as toxic by-products but also as important signaling molecules playing a key role in fruit development and ripening. To counteract the negative effects of ROS, plants and fruit own multiple ROS-scavenging mechanisms aiming to ensure a balanced ROS homeostasis. In the present study, changes in specific ROS (i.e. H2O2) as well as enzymatic (SOD, CAT, POX, APX) and non-enzymatic (phenylpropanoids, carotenoids and ascorbate) ROS-scavenging systems were investigated along four different stages of nectarine (cv. 'Diamond Ray') fruit development and ripening (39, 70, 94 and 121 DAFB) both at the metabolic (28 individual metabolites or enzymes) and transcriptional level (24 genes). Overall, our results demonstrate a complex ROS-related transcriptome and metabolome reprogramming during fruit development and ripening. At earlier fruit developmental stages an increase on the respiration rate is likely triggering an oxidative burst and resulting in the activation of specific ethylene response factors (ERF1). In turn, ROS-responsive genes or the biosynthesis of specific antioxidant compounds (i.e. phenylpropanoids) were highly expressed or accumulated at earlier fruit developmental stages (39-70 DAFB). Nonetheless, as the fruit develops, the decrease in the fruit respiration rate and the reduction of ERF1 genes leads to lower levels of most non-enzymatic antioxidants and higher accumulation of H2O2. Based on available literature and the observed accumulation dynamics of H2O2, it is anticipated that this compound may not only be a by-product of ROS-scavenging but also a signaling molecule accumulated during the ripening of nectarine fruit.
      原文链接:
    • NSTL
    • Elsevier

    Potassium and melatonin-mediated regulation of fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) activity improve photosynthetic efficiency, carbon assimilation and modulate glyoxalase system accompanying tolerance to cadmium stress in tomato seedlings

    Siddiqui, Manzer H.Mukherjee, SoumyaKumar, RiteshAlansi, Saleh...
    17页
    查看更多>>摘要：The mechanism of the combined action of potassium (K) and melatonin (Mel) in modulating tolerance to cad-mium (Cd) stress in plants is not well understood. The present study reveals the synergistic role of K and Mel in enhancing physiological and biochemical mechanisms of Cd stress tolerance in tomato seedlings. The present findings reveal that seedlings subjected to Cd toxicity exhibited disturbed nutrients balance [nitrogen (N) and potassium (K)], chlorophyll (Chl) biosynthesis [reduced 3-aminolevulinic acid (3-ALA) content and 3-amino-levulinic acid dehydratase (3-ALAD) activity], pathway of carbon fixation [reduced fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) activity] and photosynthesis process in tomato seed-lings. However, exogenous application of K and Mel alone as well as together improved physiological and biochemical mechanisms in tomato seedlings, but their combined application proved best by efficiently improving nutrient uptake, photosynthetic pigments biosynthesis (increased Chl a and b, and Total Chl), carbon flow in Calvin cycle, activity of Rubisco, carbonic anhydrase activity, and accumulation of total soluble carbo-hydrates content in seedlings under Cd toxicity. Furthermore, the combined treatment of K and Mel suppressed overproduction of reactive oxygen species (hydrogen peroxide and superoxide), Chl degradation [reduced chlorophyllase (Chlase) activity] and methylglyoxal content in Cd-stressed tomato seedlings by upregulating glyoxalase (increased glyoxalase I and glyoxalase II activity) and antioxidant systems (increased ascorbate-glutathione metabolism). Thus, the present study provides stronger evidence that the co-application of K and Mel exhibited synergistic roles in mitigating the toxic effect of Cd stress by increasing glyoxalase and antioxidant systems and also by improving photosynthetic efficiency in tomato seedlings.
      原文链接:
    • NSTL
    • Elsevier

    Microbiome-wide association studies between phyllosphere microbiota and ionome highlight the beneficial symbiosis of Lactococcus lactis in alleviating aluminium in cassava

    Xu, HaoranWei, YunxieZhang, JiachaoShi, Haitao...
    9页
    查看更多>>摘要：The phyllosphere is one of the most abundant habitats for global microbiota. The ionome is the composition of mineral elements in plants. The correlation between phyllosphere microbiota and the ionome remains elusive in plants, especially in the most important tropical crop cassava. In this study, microbiome-wide association studies (MWASs) of thirty varieties were performed to reveal the association between phyllosphere microbiota and ionomic variations in cassava. Annotation of metagenomic species identified some species that were significantly correlated with ionomic variations in cassava. Among them, Lactococcus lactis abundance was negatively associated with leaf aluminium (Al) levels but positively related to leaf potassium (K) levels. Notably, both the reference and isolated L. lactis showed strong binding capacity to Al. Further bacterial transplantation of isolated L. lactis could significantly decrease endogenous Al levels but increase K levels in cassava, and it can also lead to increased citric acid and lactic acid levels as well as higher transcript levels of K uptake-related genes. Taken together, this study reveals the involvement of phyllosphere microbiota in ionomic variation in cassava, and the correlation between L. lactis abundance and Al and K levels provides novel insights into alleviating Al accumulation and promoting K uptake simultaneously.
      原文链接:
    • NSTL
    • Elsevier

    Evaluation of maize/peanut intercropping effects on microbial assembly, root exudates and peanut nitrogen uptake

    Khan, Muhammad UmarLin, XiaoqinLin, ZhiminLin, Sheng...
    9页
    查看更多>>摘要：Legume/cereal intercropping has been widely studied within ecosystem function, owing to its overyield potential, predominantly by dinitrogen (N2) fixation. In our 2-year peanut/maize intercropping field experiment, land equivalent ratio (LER) showed an average yield-increase by 41.48%. Performance index of intercropped peanut (IP) functional leaves exhibited significant improvement (2.02-fold). Moreover, IP increased dry nodule weight by 58.82% as compared to mono-cropped. Also, the ratio of nodules to aboveground biomass in IP reduced by 65.8%. In pot experiment, higher urease activity was found in rhizosphere (22.73%). The abundance of Rhizobium and ni integral H gene in the rhizosphere of IP were significantly enhanced by 71.91% and 208%, respectively. To analyze root exudates, we performed hydroponic coculture, the proportion of total isoflavonoids in peanut root exudates were increased distinctly by 22.4%. Our findings certainly helped in filling one the information gaps, that how intercropping increases nitrogen fixation in rhizosphere. Lastly, it can further facilitate to understand functional significance of intercropping system for agricultural ecological sustainability and efficient resource utilization.
      原文链接:
    • NSTL
    • Elsevier

    Identification of trehalose-6-phosphate synthase (TPS)-coding genes involved in flowering induction of Liliumx formolongi

    Zhang, QianZhang, MengZhao, Yu-QianHu, Hao...
    11页
    查看更多>>摘要：Lilium x formolongi, a facultative long-day (LD) plant, can complete the floral transition within one year after sowing under LD conditions. In addition to the photoperiod, the molecular mechanisms by which other flowering regulators, such as sugar, participate in juvenile development and flowering induction in L. x formolongi remain elusive. Therefore, based on the investigation of seedling development under different day length conditions, we explored the growth and nonstructural carbohydrate (NSC) contents of leaves and underground bulbs. Furthermore, the expression profiles of trehalose-6-phosphate synthase (TPS)-coding genes, LfTPSs, and miR156 were also determined. Three putative LfTPS genes, LfTPS1, LfTPS3 and LfTPS5, displayed high expression levels at the juvenile vegetative stage under different day length conditions. Among them, LfTPS1 maintained gradually elevated expression until the visible bud stage under short-day (SD) conditions. Additionally, the expression levels of LfTPS3 and LfTPS5 increased with the exogenous sucrose concentration. In contrast, the expression of miR156 rapidly decreased under the same sucrose treatments. Overexpression of LfTPS1/3/5 hastened flowering and the decline in miR156 expression levels to varying degrees in transgenic Arabidopsis. Taken together, the results demonstrate that LfTPS1, LfTPS3 and LfTPS5 modulate both juvenile vegetative development and flowering induction controlled by sugars in L. x formolongi.
      原文链接:
    • NSTL
    • Elsevier

    Comparative transcriptomic analysis reveals genes related to the rapid accumulation of oleic acid in Camellia chekiangoleosa, an oil tea plant with early maturity and large fruit

    Wang, ZhongweiHuang, BinYe, JinshanHe, Yichang...
    10页
    查看更多>>摘要：Camellia chekiangoleosa has a higher oleic acid content and a shorter reproductive cycle than typical oil tea plants. It was intensively sampled over six C. chekiangoleosa seed development stages. The content of fatty acids determined by GC showed that the accumulation of fatty acids gradually increased from the S1 to S5 stages, and the maximum concentration was reached in S5. Then, fatty acids declined slightly in S6. The main fatty acid component showed the same accumulation trend as the total fatty acids, except linolenic acid, which remained at a low level throughout seed developmental stages. Changes in the expression of fatty acid accumulation-related genes were monitored using second-generation and SMRT full-length transcriptome sequencing. Finally, 18.92 G accurate and reliable data were obtained. Differential expression analysis and weighted coexpression analysis revealed two "gene modules" significantly associated with oleic acid and linoleic acid contents, and the high expression of ENR, KAS I, and KAS II, which accumulate substrates for oleic acid synthesis, was thought to be responsible for the rapid accumulation of fatty acids in the early stage. The rapid increase in fatty acids in the second stage may be closely related to the synergy between the high expression of SAD and low expression of FAD2. In addition, many transcription factors, such as ERF, GRAS, GRF, MADS, MYB and WRKY, may be involved in the fatty acid synthesis. Our data provide a rich resource for further studies on the regulation of fatty acid synthesis in C. chekiangoleosa.
      原文链接:
    • NSTL
    • Elsevier

    Comparative proteome profiling of susceptible and resistant rice cultivars identified an arginase involved in rice defense against Xanthomonas oryzae pv. oryzae

    Gupta, RaviMin, Cheol WooSon, SeungminLee, Gi Hyun...
    10页
    查看更多>>摘要：Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is one of the major threats to rice productivity. Yet, the molecular mechanism of rice-Xoo interaction is elusive. Here, we report comparative proteome profiles of Xoo susceptible (Dongjin) and resistant (Hwayeong) cultivars of rice in response to two-time points (3 and 6 days) of Xoo infection. Low-abundance proteins were enriched using a protamine sulfate (PS) precipitation method and isolated proteins were quantified by a label-free quantitative analysis, leading to the identification of 3846 proteins. Of these, 1128 proteins were significantly changed between mock and Xoo infected plants of Dongjin and Hwayeong cultivars. Based on the abundance pattern and functions of the identified proteins, a total of 23 candidate proteins were shortlisted that potentially participate in plant defense against Xoo in the resistant cultivar. Of these candidate proteins, a mitochondrial arginase-1 showed Hwayeong specific abundance and was significantly accumulated following Xoo inoculation. Overexpression of arginase 1 (OsArg 1) in susceptible rice cultivar (Dongjin) resulted in enhanced tolerance against Xoo as compared to the wild-type. In addition, expression analysis of defense-related genes encoding PR1, glucanase I, and chitinase II by qRT-PCR showed their enhanced expression in the overexpression lines as compared to wild-type. Taken together, our results uncover the proteome changes in the rice cultivars and highlight the functions of OsARG1 in plant defense against Xoo.
      原文链接:
    • NSTL
    • Elsevier