查看更多>>摘要：Adaptation of plants to environmental changes involves the mechanisms of long-distance signaling. In characean algae, these mechanisms comprise the propagation of action potential (AP) and the rotational cytoplasmic streaming acting in cooperation with light-dependent exchange of ions and metabolites across the chloroplast envelope. Both excitability and cyclosis exert conspicuous effects on photosynthetic activity of chloroplasts but possible influence of cyclosis arrest on the coupling of AP stimulus to photosynthetic performance remained unexplored. In this study, fluidic interactions between anchored chloroplasts were allowed or restricted by illuminating the whole internode or a confined cell area (2 mm in diameter), respectively. Measurements of chlorophyll fluorescence parameters (F ' and Fm') in cell regions located close to calcium crystal depositions revealed that the AP generation induced long-lasting Fm' oscillations that persisted in illuminated cells. The AP generation often induced the F ' oscillations, whose number diminished upon the transfer of internodal cells from total to local background light. The results indicate that the AP-induced changes in photosynthetic parameters, F' in particular, have a complex origin and comprise the internal processes caused by the elevation of stromal Ca2+ concentration in the analyzed chloroplasts and the stages related to ion and metabolite exchange mediated by cytoplasmic streaming. It is supposed that the composition of flowing cytoplasm is heterogeneous due to the spatial alteration of calcified and noncalcified cell sites, but this heterogeneity is enhanced and can be visualized after the transient cessation and restoration of cytoplasmic streaming.
查看更多>>摘要：Waterlogging is a serious threat to agriculture that is expected to become more common due to climate change. It is well established that many plants are susceptible to waterlogging, including crops such as rapeseed. To investigate the responses and tolerance to waterlogging of the re-emerging oilseed crop camelina (Camelina sativa), camelina lines of different geographical origins were subjected to waterlogging. Camelina was very sensitive to waterlogging at vegetative growth stages, with a relatively short treatment of 4 days proving lethal for the plants. A treatment duration of 2 days resulted in growth inhibition and lower yields and was used to study the response of 8 different camelina lines to waterlogging at two different vegetative growth stages before bolting. Generally, younger plants (7-9 leaves) were more sensitive than older plants (15-16 leaves). In addition to morphological and agronomic traits, plants were phenotyped for physiological parameters such as chlorophyll content index and total antioxidant capacity of the leaves, which showed significant age-dependent changes due to waterlogging. These results underpin that waterlogging during the vegetative phase is a serious threat to camelina, which needs to be addressed by identifying and establishing tolerance to excess water to harness camelina's potential as a climate-smart crop.
查看更多>>摘要：Polyamines (PAs) are natural active compounds having more than two amino groups that play important roles in many physiological and developmental processes in plants. The purpose of this research was to see how foliar polyamine spray affected growth and photosynthetic indices, as well as secondary metabolites and antioxidant activity of the aqueous and methanolic extracts of pot marigold (Calendula officinalis L.). The experiment lasted for three months and was arranged in a randomized complete design with four replications. Three separate concentrations (0.5, 1 and 2.5 mM) of spermine (SPM), spermidine (SPD), and putrescine (PUT) were sprayed at four/five fully expanded leaf stage and some physiochemical attributes were evaluated. The treatments caused a significant increase in morphological and photosynthetic parameters and total oil. There were also significant variations in total phenolic and flavonoid content. Compared to other polyamines, 1 mM SPD foliar spraying showed the greatest effect. Furthermore, the highest antioxidant capacity (DPPH* scavenging assay, ferric reducing antioxidant power (FRAP), Trolox equivalent antioxidant capacity (TEAC) and beta-carotene bleaching activity) was observed in the 1 mM SPD treatment. The results showed that the calendula essential oils (EOs) were rich in sesquiterpenes hydrocarbons (55.92-95.94%), with c-Cadinene and d-Cadinene as the major sesquiterpenes in the EOs. Also, the flowers were rich sources of carotenoids (lutein, flavoxanthin and luteoxanthin) following polyamines application. Hence, it can be inferred that polyamines specially spermidine would find a wide range of application in pharmaceutical industries due to its impact on antioxidant properties of phenolic and flavonoid compounds.
查看更多>>摘要：The tea plant (Camellia sinensis) is one of the three major beverage crops in the world with its leaves consumption as tea. However, it can hyperaccumulate fluoride with about 98% fluoride deposition in the leaves. Our previously studies found that cell wall proteins (CWPs) might play a central role in fluoride accumulation/detoxification in C. sinensis. CWP is known to be glycosylated, however the response of CWP N-glycosylation to fluoride remains unknown in C. sinensis. In this study, a comparative N-glycoproteomic analysis was performed through HILIC enrichment coupled with UPLC-MS/MS based on TMT-labeling approach in C. sinensis leaves. Totally, 237 N-glycoproteins containing 326 unique N-glycosites were identified. 73.4%, 18.6%, 6.3% and 1.7% of these proteins possess 1, 2, 3, and >= 4 modification site, respectively. 93.2% of these proteins were predicted to be localized in the secretory pathway and 78.9% of them were targeted to the cell wall and the plasma membrane. 133 differentially accumulated N-glycosites (DNGSs) on 100 N-glycoproteins (DNGPs) were detected and 85.0% of them exhibited upregulated expression after fluoride treatment. 78.0% DNGPs were extracellular DNGPs, which belonged to CWPs, and 53.0% of them were grouped into protein acting on cell wall polysaccharides, proteases and oxido-reductases, whereas the majority of the remaining DNGPs were mainly related to Nglycoprotein biosynthesis, trafficking and quality control. Our study shed new light on the N-glycoproteome study, and revealed that increased N-glycosylation abundance of CWPs might contribute to fluoride accumulation/detoxification in C. sinensis leave.
查看更多>>摘要：Arundo donax L. is an invasive species that has been recently employed for biomass production due to its wellknown ability to colonize harsh environment. Based on previous observations, the present study investigated the potential role of phenylpropanoids and class III peroxidases to confer adaptation through biochemical and transcriptomic analysis in A. donax after Na+ and P excess supply, both in single stress and in combination, and after growth at low P level. The levels of hydrogen peroxide, flavonoids (i.e., quercetin, apigenin and kaempferol derivatives) and the activity of class III peroxidases, as well as the expression of several genes encoding for their enzymes involved in their biosynthesis, increased when Na+ was supplied in combination with P. These results suggest that those biomolecules are involved in the response of A. donax, to the presence of +Na and P in the soil. Moreover, even though at the sampling time no significant accumulation of lignin has been determined, the trend of accumulation of such metabolite and most of all the increase of several transcripts involved in its synthesis was found. This work for the first time indicates the need for further investigation devoted to elucidating whether the strengthening of cell walls via lignin synthesis is one of the mechanisms used by A. donax to adapt to harsh environments.
查看更多>>摘要：Root penetration ability is critical for plant growth and development. When roots encounter soil impedance, hormones are activated that affect cells and tissues, leading to changes in root morphology and configuration that often increase root penetration ability. Factors, such as root system architecture, root anatomic traits, rhizosphere exudation and root-induced phytohormones, influencing root penetration ability and how they affect plant performance under soil impedance were summarized. Root penetration ability affects plant capturing water and nutrients, and thus determines plant performance and productivity in adverse environments. Great efforts have been made in searching for the underlying mechanisms of root penetration ability, and tools have been developed for phenotyping variability in root penetration ability. Therefore, with the continued development of agroecosystems based on the advocated low input costs and controlled tillage, crops or genotypes of a crop species with stronger root penetration ability may have the potential for developing new varieties with enhanced adaptation and grain yield under mechanical impedance in soil.
NSTL
Elsevier