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Spectrochimica acta
Pergamon
Spectrochimica acta

Pergamon

1386-1425

Spectrochimica acta/Journal Spectrochimica acta
正式出版
收录年代

    A novel lysosome-located fluorescent probe for highly selective determination of hydrogen polysulfides based on a naphthalimide derivative

    Tian, MeijuXu, JunhongMa, QiujuanLi, Linke...
    10页
    查看更多>>摘要：Hydrogen polysulfides (H2Sn, n > 1) belongs to sulfane sulfur in the reactive sulfur species (RSS) family and plays a significant regulatory role in organisms. Highly selective and lysosome-located probes for detecting hydrogen polysulfides are rare. Thus, it is important to develop a technique to detect the changes of H2Sn level in lysosomes. In this work, a lysosome-targeting fluorescent probe for H2Sn was designed and developed based on a naphthalimide derivative. 4-Hydroxynaphthalimide was selected as the fluorescent group and 2-chloro-5-nitrobenzoate group was used as a specific recognition unit for H2Sn. A morpholine unit was chosen as a lysosome-located group. In the absence of H2Sn, the fluorescent probe exhibited almost no fluorescence. In the presence of H2Sn, the fluorescent probe showed strong fluorescence owing to H2Sn-mediated aromatic substitution-cyclization reactions. The fluorescence emission intensity at 548 nm of the probe showed a good linear relationship toward H2Sn in the range of 2.0 x 10(-7) - 9.0 x 10(-5) mol.L-1, and the detection limit was found to be 1.5 x 10(-7) mol.L-1. The probe possessed a wide work range of pH, including the pH of physiological environment, and high selectivity for H2Sn. There are almost no cytotoxicity and the ability of detecting endogenous and exogenous H2Sn, in lysosomes. These results indicate that the fluorescent probe can provide a good tool for intracellular and extracellular detection of H2Sn. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    Colorimetric detection of polycyclic aromatic hydrocarbons by using gold nanoparticles

    Ma, Chih-MingLin, Li-ChiehChuang, Kai-JenHong, Gui-Bing...
    8页
    查看更多>>摘要：The existence and content of polycyclic aromatic hydrocarbons (PAHs) in the environment have gradually received attention because PAHs are widely detected in many sources. Therefore, an effective detection method for PAHs is necessary for further treatment. This study proposes a novel colorimetric detection method based on AuNPs to determine the contents of phenanthrene (Phe) and pyrene (Pyr). Trisodium citrate was used as a reducing agent to synthesize gold nanoparticles, and ammonium nitrate (NH4NO3) was added as a reactant to detect the analyte content. Some assay parameters, such as the concentration of NH4NO3 solution, the volume of NH4NO3 solution, the concentration of MES buffer solution, the volume of MES buffer solution, and the reaction time influenced the analyte detection ability of AuNPs and were optimized. The limits of detection for Phe and Pyr are 0.06 mg/L and 0.087 mg/L, respectively. In addition, the detection method has good selectivity and anti-interference ability for the target analytes. This colorimetric method was used to detect target analytes in real water (tap water and mineral water) with acceptable results. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    The role of pro- and antiangiogenic factors in angiogenesis process by Raman spectroscopy

    Kopec, M.Abramczyk, H.
    10页
    查看更多>>摘要：Raman spectroscopy and Raman imaging are powerful techniques to monitor biochemical composition around blood vessel. The aim of this study was to understand the role of pro- and antiangiogenic factors in angiogenesis process. Raman imaging and Raman single spectrum measurements allow the diagnosis of cancer biochemical changes in blood vessel based on several biomarkers simultaneously. We have demonstrated that Raman imaging combined with statistical methods are useful to monitoring pro-and antiangiogenic factors responsible for angiogenesis process. In this work Raman markers of proangiogenic and antiangiogenic factors were identified based on their vibrational signatures. Obtained results can help understand how growing tumor create its vascular system. (C) 2021 The Author(s). Published by Elsevier B.V.
      原文链接:
    • NSTL
    • Elsevier

    Folic acid functionalized molybdenum oxide quantum dots for the detection of Cu2+ ion and alkaline phosphatase via fluorescence turn off-on mechanism

    Kateshiya, Mehul R.Malek, Naved I.Kailasa, Suresh Kumar
    11页
    查看更多>>摘要：The assay of alkaline phosphatase (ALP) plays a key role in the diagnosis of various diseases. Herein, folic acid functionalized molybdenum oxide quantum dots (FA-MoOx QDs) are explored as fluorescence "turn-off and on" probes for assaying of Cu2+ ion and ALP, respectively. This fluorescence sensing strategy was based on the quenching of emission peak of FA-MoOx QDs at 445 nm by Cu2+ ion, followed by restoring of emission peak selectively with ALP. Based on the quenching and restoring of FA-MoOx QDs emission intensity, quantitative assay was developed for the detection of Cu2+ ion (0.20 - 500 mu M) and ALP (0.06 - 150 U/L) with detection limits of 29 nM and 0.026 U/L, respectively. The developed FA-MoOx QDs-based fluorescence ''turn- off and on" strategy exhibited satisfactory results for assaying of ALP in biofluids. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    Simple SPR-based colorimetric sensor to differentiate Mg2+ and Ca2+ in aqueous solutions

    Amirjani, AmirmostafaSalehi, KimiaSadrnezhaad, S. K.
    8页
    查看更多>>摘要：L-tryptophan functionalized AgNPs were successfully fabricated using a one-pot synthesis method and assessed as a colorimetric probe for rapid and accurate determination of Mg2+ ions. The developed sensor showed a selective response towards Mg2+ with no interference from Ca2+ in the wide concentration range of 1-200 mu M. The sensor's response was optimized in the pH range of 9-10, which can be attributed to the protonation of amine groups and their interaction with Mg2+ ions. The stability and selectivity of the sensor were examined in different salt (NaCl) and other metal ions, respectively. The L-tryptophanAgNPs sensor detected Mg2+ with the limit of detection of 3 mu M, which is way lower than the concentration range of magnesium in human serum (0.75-1.05 mM). The recovery values of the developed sensor were in the range of 96-102% for the determination of Mg2+ in urine samples. The obtained performances proved the potential application of the developed sensor for clinical diagnostic of Mg2+ ions where an accurate and rapid response is needed. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    Benzoindoxazine derivatives containing carbazole for detection of CN- and its application in plant seed extracts and cell imaging

    Pan, WeiChen, Guo-GuoZhang, Zhen-YouCao, Xiao-Qun...
    16页
    查看更多>>摘要：Cyanide (CN-) is a highly toxic compound that exists in many substances and is harmful to the environment and human health. Therefore, it is of great significance to develop excellent CN- ion probes, especially solvent-induced on-off fluorescent probes. Based on the condensation reaction of indolo[2,1-b] [1,3]oxazine molecules with aldehydes, probes (E)-13a-(2-(9-ethyl-9H-carbazol-3-yl)vinyl)-14,14-dime thyl-10-nitro-13a,14-dihydro-8H-benzo[e]benzo[5,6][1,3]oxazino[3,2-a]indole (NCO) and (E)-13a-(2-(9-benzyl-9H-carbazol-3-yl)vinyl)-14,14-dimethyl-10-nitro-13a,14-dihydro-8H-benzo[e]benzo[5,6][1,3] oxazino[3,2-a]indole (NBO) were synthesized to detect CN-. Compared with other cyanogen ion probes, NCO and NBO have special carbazole ring structures and large conjugate systems. When CN(-)is added to the probe-detection solution, color changes that are visible to the naked eye can occur. The UV-vis spectrum test using differential spectroscopy shows that the probe (i) has excellent solvent-induced switching characteristics and stability (CH3OH-H2O) and (ii) high selectivity, anti-interference ability, and sensitivity for the detection of CN-. The fluorescence limit of detections (LODs) are 1.05 mu M for NCO and 1.34 mu M for NBO. The UV LODs are 0.83 mu M for NCO and 0.87 mu M for NBO. Fluorescence spectroscopy shows that the probe has remarkable fluorescence properties. Fluorescence titration experiments, liver cancer cell (Hep G2) imaging, and cytotoxicity experiments all show that the probes have high biocompatibility, low toxicity, high cell permeability, and high sensitivity for the detection of CN- in cells. In addition, NCO and NBO have been successfully used for the detection of cyanogenic glycosides in the seeds of ginkgo, crabapple, apple, and cherry. Test strips were fabricated to detect CN-. After adding CN-, the color of the test strip changed significantly-from brown to light yellow; thus, the test strips have a high application value in the fields of drug quality control, drug safety testing, and pharmacological research. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    Optimization of experimental conditions by surface enhanced Raman Scattering (SERS) spectroscopy with gold nanoparticles suspensions

    Berge, MarionLegrand, Francois-XavierTfayli, AliLe, Laetitia Minh Mai...
    9页
    查看更多>>摘要：Surface Enhanced Raman Scattering (SERS) spectroscopy is a rapid and innovative analysis technique involving metallic nanoparticles (NPs ). The interaction between NPs and norepinephrine gives an exaltation of the Raman signal under certain experimental conditions. The control of the signal exaltation, crucial for sensitive analyses, remains one of the main limitations of this technique. The aim of this work is to optimize the exaltation conditions for an optimal SERS signal at two concentrations of norepinephrine (NOR) and spherical gold NPs in suspension. This first work will fix the optimal experimental conditions essential for the development of robust discriminant and quantitative analysis of catecholamine. Two complete 3-factors 3-levels experiment designs were performed at 20 mu g.mL(-1) and 100 mu g.mL(-1) norepinephrine concentrations, each experiment being repeated 3 times. The optimization factors were the process of synthesis (variation of the quantity of gold and citrate used for the three synthesis SA, SB and SC) and HCl (0.3 M, 0.5 M, 0.7 M) as well as the volume ratio of NPs and norepinephrine (0.5, 2, 3.5) for SERS acquisition. Spectral acquisitions were performed with a handheld Raman spectrometer with an excitation source at 785 nm. For each sample, 31 acquisitions were realized during 3 s every 8 s. The optimization parameter was the intensity of the characteristic band of norepinephrine at 1280 cm(-)(1). A total of 5,042 spectra were acquired and the pre-treatment selected for all spectra was asymmetric least square combined to a smoothing of Savistsky Golay (ALS - SG). The optimal contact time between norepinephrine and NPs depends on the experimental conditions and was determined for each experiment according to the mean intensity between the three replicates. After interpretation of the experimental designs, the optimal conditions retained were the quantity of gold corresponding to SA and the HCl-concentration 0.7 M for the two concentrations of norepinephrine. Indeed, the optimal volume ratio depend on the NOR concentration. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    Colorimetric detection of ATP by inhibiting the Peroxidase-like activity of carbon dots

    Huang, NaWen, JiahuiYi, DanyangWei, Zixuan...
    8页
    查看更多>>摘要：Adenosine triphosphate (ATP) is the main energy currency for cells and an important biomolecule involved in cellular reactions, whose abnormal levels are closely related to physical disease, thus it is extremely important to establish a convenient, fast and simple ATP monitoring method. Toward this end, we developed a facile method for colorimetric detection of ATP on the basis of the inhibiting effect of ATP on the peroxidase-like activity of carbon dots (CDs). The detection principle of this method was utilizing the peroxidase-like activity of CDs, which catalyze the oxidation of 3,3'5,5'-tetramethylbenzi dine (TMB) by H2O2 to generate blue products. However, the introduction of ATP in the system can inhibit the generation of blue products, so ATP can be colorimetric detected. This method exhibited high sensitivity with a detection limit of 34 nM and a wide linear range (0.050-2.0 mu M). The as-proposed colorimetric ATP sensor was capable of detecting ATP in real samples accurately. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    Spectropathologic endorsement of ocular carotenoids for early detection of diabetic retinopathy

    Singh, Anang KumarKarjee, HimadriGhosh, SambuddhaChatterjee, Jyotirmoy...
    8页
    查看更多>>摘要：Diabetic retinopathy (DR) is a common health concern. Unfortunately, the metabolic pathway causing DR is yet to be understood. The carotenoid level in the human body is known to protect the health of the eyes. In this work, resonance Raman spectroscopy and multivariate analysis of the spectral data of human serum are reported as next-generation spectropathologic tools to detect retinal degeneration efficiently. The proposed technique shows promise by endorsing ocular carotenoids as a critical biomarker for such pathosis. Furthermore, the multivariate analysis of the spectral data distinguishes between two different stages of the disease. The machine learning algorithm is used to estimate a significant accuracy of 94% of the proposed model for the classification. As the carotenoid level can be controlled by dietary intake, we believe that the reported results also indicate a therapeutic role of the same in DR. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier

    Monitoring of parathion methyl using a colorimetric gold nanoparticle-based acetylcholinesterase assay

    Liu, BichengWu, LiangmingPeng, ZoujunWu, Sihao...
    8页
    查看更多>>摘要：A colorimetric gold nanoparticles (AuNPs)-based acetylcholinesterase (AChE) assay was designed for the first time to measure the concentration of parathion-methyl (PM) in lake water samples. In this assay, the analyte PM inhibited the hydrolysis of acetylthiocholine (ATCh) by AChE, preventing the formation of thiocholine (TCh) that would otherwise react with the AuNPs catalyst and deactivate the catalyst. Therefore, in the presence of PM, the AuNPs catalyzed the oxidation of the 3,3',5,5'-tetramethylbenzidine (TMB) colorimetric indicator to oxTMB, inducing a visual color change from colorless to blue. However, in the absence of PM, AChE hydrolyzed ATCh to TCh, which then reacted with the AuNPs, preventing the oxidation of TMB to oxTMB and rendering the solution colorless. Therefore, the change in the color of the analyte solution indicated the presence of PM, and the absorbance of the resulting solution was measured by UV-Vis spectroscopy to calculate the concentration of PM after generation of a calibration curve. This method was then employed using the smartphone app Color Picker, which converted the color information from the photos of the solution into digital red (R), green (G), and blue (B) values. The ratio of green (G) to blue (B) (G/B) was then plotted against the corresponding concentration to calculate the standard curve, whose regression equation was expressed by y = -0.012x + 1.02 (ng/mL), and the coefficient of determination (R-2) was 0.97. In addition, this method was also used to determine the amount of PM in real lake water samples with recovery of 90.2-133.3%. (C) 2021 Elsevier B.V. All rights reserved.
      原文链接:
    • NSTL
    • Elsevier