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Russian Journal of Nematology
Moscow
Russian Journal of Nematology

Moscow

0869-6918

Russian Journal of Nematology/Journal Russian Journal of NematologyISTPSCI
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    DNA barcoding and phylogeny of some cyst nematode species from the genus Heterodera (Tylenchida: Heteroderidae) parasitising monocots

    Swart, Antoinettedel Prado Vera, Ignacio CidMaafi, Zahra TanhaChizhov, Vladimir N....
    8页
    查看更多>>摘要：The genus Heterodera presently contains 87 valid species, of which five species: H. bifenestra, H. cardiolata, H. cyperi, H. goldeni and H. sacchari are molecularly characterised in this study. Using molecular criteria, we also distinguished five putative new species: Heterodera sp. 1-sp. 5 from the Afenestrata, Cardiolata, Bifenestra and Sacchari groups. A total of 16 new ITS rRNA, four new D2-D3 expansion segments of 28S rRNA and 23 new partial COI gene sequences were obtained from 14 cyst nematode populations collected from nine countries: Ghana, Iran, Mexico, Pakistan, Russia, Spain, Tanzania, The Netherlands and Ukraine. Phylogenetic relationships within the genus Heterodera are presented using these three gene fragments. The study confirmed the conclusions that each cyst nematode species has a unique COI sequence or DNA barcode that enables its identification and separation from all other species.
      原文链接:
    • NSTL
    • Russian Acad Sci, Inst Parasitology

    Morphological and molecular characterisation of Boleodorus volutus Lima & Siddiqi, 1963 from South Africa with the first SEM observations of the species

    Shokoohi, Ebrahim
    9页
    查看更多>>摘要：During a survey on plant-parasitic nematodes in South Africa, a population of Boleodorus was recovered in association with grasses. This species characterised by a conical lip region lacking incisures. Stylet length 9-11 mu m, lateral field with four incisures, post-vulval sac less than the corresponding body diameter. Tail conically elongated, often hooked at the end, 51-58 mu m long. The nblast analysis based on the D2-D3 segment of 28S rDNA of the South African population of B. volutus, revealed 98% similarity to the North American B. volutus (MT994501). The phylogenetic analysis put the South African population of B. volutus together with the other B. volutus and unidentified Boleodorus with a 100 posterior probability support. In addition, the SEM photographs are provided for the first time for B. volutus. This is the first complete report of this species in South Africa.
      原文链接:
    • NSTL
    • Russian Acad Sci, Inst Parasitology

    High initial population densities of Meloidogyne graminicola result in loss of resistance and tolerance of recombinant inbred lines (RIL) of Asian rice (Oryza sativa)

    Galeng-Lawilao, JudithKumar, ArvindDe Waele, Dirk
    16页
    查看更多>>摘要：A glasshouse experiment was conducted to examine the effect of a range of high initial populations densities (Pi = 25,000, 50,000 and 100,000 second-stage juveniles (J2) plant(-1)), equal to an initial pathogen pressure of 5, 10 and 20 J2 (g soil)(-1)), respectively, of a Meloidogyne graminicola population from Batangas (Philippines) on recombinant inbred lines (RIL) that had been classified as either (partially) resistant or tolerant to a lower initial pathogen pressure (1 J2 (g soil)(-1)) of this M. graminicola population. These RIL were derived from a cross between the Asian rice (Oryza sativa) genotypes 'IR78877-208-B-1-2' (resistant and tolerant donor parent) and either 'IR64' or 'Dinorado' (susceptible and highly sensitive recipient parents). Pi resulted (with few exceptions) in M. graminicola root population densities > 10,000 J2 (root system)(-1) and > 1,000 J2 (g roots)(-1), and in root gall indices >= 4.5 in the RIL and 'IR78877-208-B-1-2'. Based on the high final nematode population densities and root gall indices, the RIL and 'IR78877-208-B-1-2' cannot be considered as either resistant or partially resistant to M. graminicola. The effect of Pi on plant growth and yield varied among the variables measured and among the RIL. Plant height was least affected but root and shoot weight, number of panicles plant(-1) and % filled grains plant(-1) were significantly reduced. If a % yield reduction of < 10% and 10-20% (compared with non-infected control plants) is used as an indication of tolerance or less sensitivity, respectively, to M. graminicola infection, then none of the RIL and 'IR78877-208-B-1-2' can be considered as either tolerant or less sensitive to M. graminicola. These results underline the necessity when selecting donor parents to introgress either resistance/partially resistance or tolerance/less sensitivity to M. graminicola infection into Asian rice breeding lines with good agronomic characteristics to test the host response of these candidate donor parents under a wide range of environmental conditions that may influence the resistance and/or sensitivity of these candidate donor parents.
      原文链接:
    • NSTL
    • Russian Acad Sci, Inst Parasitology

    First report of Steinernema borjomiense Gorgadze et al., 2018 (Rhabditida: Steinernematidae) from Iran and its efficacy against two exotic invasive forest pests in laboratory condition

    Ghavamabad, Reihaneh GholamiTalebi, Ali AsgharMehrabadi, MohammadFarashiani, Mohammad Ebrahim...
    15页
    查看更多>>摘要：The entomopathogenic nematode, Steinernema borjomiense, was isolated from north Iran, and identified herein based upon morphological and molecular data. The Iranian isolate of this species is characterised by its 645-910 mu m long infective juveniles, having brown-golden spicules 52-67 mu m long in first, and 30-51 mu m long in second generation males. The pathogenicity of the recovered population of S. borjomiense was evaluated on the larvae of two exotic invasive forest pests in north Iran, the box tree moth (BTM), Cydalima perspectalis, and the fall webworm (FWW), Hyphantria cunea, under laboratory conditions. The results indicated that the lethal concentration 50 (LC50) values of nematode were 60.2 and 180.3 IJ larva(-1) on fifth and fourth instar larvae of BTM; and 96.7 and 213.8 IJ larva(-1) on fifth and fourth instar larvae of FWW, respectively, after 48 h at 25 degrees C and 60% relative humidity (RH). Together, the present results corroborated the efficacy of this isolate for biocontrolling BTM and FWW in laboratory conditions.
      原文链接:
    • NSTL
    • Russian Acad Sci, Inst Parasitology

    Morphological and molecular characterisation of root-lesion nematodes (Pratylenchus spp.) (Rhabditida: Pratylenchidae) associated with apple in South Africa

    Knoetze, Rinusvan den Berg, Esthervan der Walt, LeneGirgan, Chantelle...
    26页
    查看更多>>摘要：Pratylenchus spp. are widely known to cause damage to apple trees. A survey was conducted in all the major pome fruit production areas in South Africa, amounting to more than 100 sampling localities. Lesion nematodes detected in the samples were molecularly identified by PCR amplification and sequencing of the D2-D3 expansion segments of 28S, as well as ITS-rDNA and the cytochrome oxidase gene of mitochondrial DNA (COI). Viable lesion nematodes were handpicked from each sample and transferred to carrot discs for in vitro propagation. A sub-sample of each population was also preserved for morphological identification to species level and taxonomic studies. Pratylenchus hippeastri was detected in most of the sampled regions, except Villiersdorp. In some instances, mixed populations of P. hippeastri, P. vulnus and P. penetrans were found, but P. hippeastri was the most abundant. Morphological and molecular studies confirmed the identity of these species.
      原文链接:
    • NSTL
    • Russian Acad Sci, Inst Parasitology

    A novel free-living marine nematode species Pseudochromadora thinaiica sp. n. (Nematoda: Desmodoridae) from the seagrass bed of Vietnam

    Zograf, Julia K.Skripova, Ekaterina R.Semenchenko, Alexander A.Viet Dung Vu...
    14页
    查看更多>>摘要：Pseudochromadora thinaiica sp. n. is described from intertidal sediment in Thi Nai Lagoon (Vietnam). Pseudochromadora thinaiica sp. n. is characterised by the combination of the following characters: a cephalic capsule subdivided into two parts (main head region and helmet-shaped labial region); six longitudinal rows of somatic setae; no precloacal supplements; boat-shaped gubernaculum with proximal end curved dorsally, buccal cavity with a large dorsal tooth and two ventrosublateral teeth. Pseudochromadora thinaiica sp. n. is most similar to P. parva Gagarin & Thanh, 2008 in the shape of gubernaculum, but differs from it by longer body, stoma armature, bigger spicules and gubernaculum, the number of postcloacal thorns, absence of sexual dimorphism in the shape of the fovea amphidialis, absence of ventral hillock with thorns. Phylogenetic relationships using 18S and 28S rDNA confirm monophyly of Pseudochromadora.
      原文链接:
    • NSTL
    • Russian Acad Sci, Inst Parasitology

    Characterisation of Rotylenchus buxophilus Golden, 1956 from the European part of Russia

    Tabolin, Sergei B.Baslerov, Roman, V
    3页
      原文链接:
    • NSTL
    • Russian Acad Sci, Inst Parasitology

    Irakli Eliava (1928-2021) In memoriam

    1页
      原文链接:
    • NSTL