首页|DNA barcoding and phylogeny of some cyst nematode species from the genus Heterodera (Tylenchida: Heteroderidae) parasitising monocots
DNA barcoding and phylogeny of some cyst nematode species from the genus Heterodera (Tylenchida: Heteroderidae) parasitising monocots
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NSTL
Russian Acad Sci, Inst Parasitology
The genus Heterodera presently contains 87 valid species, of which five species: H. bifenestra, H. cardiolata, H. cyperi, H. goldeni and H. sacchari are molecularly characterised in this study. Using molecular criteria, we also distinguished five putative new species: Heterodera sp. 1-sp. 5 from the Afenestrata, Cardiolata, Bifenestra and Sacchari groups. A total of 16 new ITS rRNA, four new D2-D3 expansion segments of 28S rRNA and 23 new partial COI gene sequences were obtained from 14 cyst nematode populations collected from nine countries: Ghana, Iran, Mexico, Pakistan, Russia, Spain, Tanzania, The Netherlands and Ukraine. Phylogenetic relationships within the genus Heterodera are presented using these three gene fragments. The study confirmed the conclusions that each cyst nematode species has a unique COI sequence or DNA barcode that enables its identification and separation from all other species.