首页|DNA barcoding and phylogeny of some cyst nematode species from the genus Heterodera (Tylenchida: Heteroderidae) parasitising monocots

DNA barcoding and phylogeny of some cyst nematode species from the genus Heterodera (Tylenchida: Heteroderidae) parasitising monocots

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The genus Heterodera presently contains 87 valid species, of which five species: H. bifenestra, H. cardiolata, H. cyperi, H. goldeni and H. sacchari are molecularly characterised in this study. Using molecular criteria, we also distinguished five putative new species: Heterodera sp. 1-sp. 5 from the Afenestrata, Cardiolata, Bifenestra and Sacchari groups. A total of 16 new ITS rRNA, four new D2-D3 expansion segments of 28S rRNA and 23 new partial COI gene sequences were obtained from 14 cyst nematode populations collected from nine countries: Ghana, Iran, Mexico, Pakistan, Russia, Spain, Tanzania, The Netherlands and Ukraine. Phylogenetic relationships within the genus Heterodera are presented using these three gene fragments. The study confirmed the conclusions that each cyst nematode species has a unique COI sequence or DNA barcode that enables its identification and separation from all other species.

28S rRNA geneCOI geneITS rRNA geneMexicomolecular phylogenyRussiaTanzaniaFORMING NEMATODESIDENTIFICATION

Swart, Antoinette、del Prado Vera, Ignacio Cid、Maafi, Zahra Tanha、Chizhov, Vladimir N.、Subbotin, Sergei A.

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ARC Plant Protect Res Inst

Colegio Postgrad

Agr Res Educ & Extens Org AREEO

Russian Acad Sci

Calif Dept Food & Agr

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2021

Russian Journal of Nematology

Russian Journal of Nematology

SCI
ISSN:0869-6918
年,卷(期):2021.29(2)
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