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Scientia horticulturae
International Society for Horticultural Science
Scientia horticulturae

International Society for Horticultural Science

0304-4238

Scientia horticulturae/Journal Scientia horticulturaeSCIISTP
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    Phenotypic and genetic characterization of "Aglione della Valdichiana": Population structure and genetic relationship analysis of a white gentle giant

    Terzaroli, NiccoloMarconi, GianpieroRussi, LuigiAlbertini, Emidio...
    9页
    查看更多>>摘要:"Aglione della Valdichiana" (Allium ampeloprasum var. holmense) is a landrace of Great Headed Garlic (GHG), an historical and healthy vegetable that has been recently rediscovered and registered in the National and Regional catalog of local varieties. It is grown in small fields in a region between Tuscany and Umbria, Italy, and due to its high market value, it is at risk of commercial frauds. The morphological traits and molecular profile of 9 local populations were evaluated together with 36 accessions of leek, garlic, wild relatives and other GHGs of different geographic origins and used as controls. The 39 morphological descriptors were able to distinguish the 9 accessions from leek and garlic, but unable to distinguish among them. The 549 AFLP (Amplified Fragment Length Polymorphism) amplicons obtained from nine primer combinations were able to clearly distinguish Aglione accessions from all the others, both by UPGMA (Unweighted Pair Group Method with Arithmetic mean) clustering and by STRUCTURE analysis, and this is due to several private AFLP bands, most from M+AGC/E+CTG primer combination. Despite its vegetative propagation, the within accession variability of Aglione is higher than expected and most likely due to somatic mutations. The genetic relationships are discussed, including the closer relationship found between Aglione and garlic vs. Aglione and leek.

    Effect of biosorptive removal of cadmium ions from hydroponic solution containing indigenous garlic peel and mercerized garlic peel on lettuce productivity

    Abu-Shahba, Mahmoud S.Mansour, Mahmoud M.Sofy, Mahmoud R.Mohamed, Heba, I...
    14页
    查看更多>>摘要:One of the most harmful trace elements is cadmium (Cd) because of its persistence and high mobility. As Cdcontamination of soils increases worldwide, techniques to reduce the lettuce plant's Cd accumulation are required. The adverse effects of Cd can be reduced or eliminated using indigenous garlic peel (IGP) and, to a greater extent, mercerized garlic peel (MGP). In addition, the adsorption capacity of garlic peel after mercerization increased compared to indigenous garlic peel. This investigation explored the efficacy of IGP or MGP on the growth characteristics, Cd concentration, and biochemical processes in Cd-stress lettuce plants using a hydroponic growing system. The results demonstrated that IGP or MGP mitigated Cd's toxicity, improving lettuce growth, reducing Cd content, translocation factor, biological accumulation factor, tolerance index, malondialdehyde, and oxidative stress in both leaves and roots. In addition, the application of IGP and MGP significantly increased the non-enzymatic and enzymatic antioxidants in leaves and roots under Cd stress. Furthermore, all treated plants displayed high expression levels of certain regulatory defense genes, UBC21, LOX, and PAL.

    Water relations after Ca, B and Si application determine fruit physical quality in relation to aquaporins in Prunus

    Quirante-Moya, FranciscoMartinez-Alonso, AlbertoLopez-Zaplana, AlvaroBarzana, Gloria...
    14页
    查看更多>>摘要:As climacteric fruits, fleshy Prunus has a short ripening period and short shelf-life after harvest. Therefore, the main problem with these fruits is its physiological parameters related to the maintenance of firmness. In this work, the effects of combined foliar sprays (Ca+B and Ca+Si) on the physical and physiological parameters of prunus fruits were studied. Also, the involvements of aquaporin expression in relation to the maintenance of water and nutrients in the fruits were determined. Foliar treatments were applied to cherry (Prunus avium L.), apricot (Prunus armeniaca), doughtnut peach (Prunus persica L. var platycarpa), and nectarine (Prunus persica L.) trees grown under commercial conditions. The effects of treatments on fresh weight seemed to be dependent on the different species, with increases in apricot by Ca+Si and in nectarines by Ca+B. The firmness in cherries and apricots was enhanced by all the treatments. Also, apricot, doughnut peach and nectarine fruits did not show changes in osmotic potential, although increases in water potential and turgor potential were observed. Increases in some aquaporins only appeared in cherry and nectarine but not in apricot and doughnut peach which is in agreement with the results obtained in the activity of aquaporins in association with the retaining of electrolytes. Therefore, the results of this work indicate that the study of water relations involving aquaporins in mature prunus fleshy fruits could be a key aspect for the maintenance of turgor.

    Tolerance strategies and factors that influence the cadmium uptake by cacao tree

    Machado Oliveira, Bruna RafaelaDe Almeida, Alex-Alan FurtadoSantos, Nayara de AlmeidaPirovani, Carlos Priminho...
    10页
    查看更多>>摘要:Cadmium (Cd) is a non-essential trace metal, highly toxic to plants, animals and humans. Its mobility in the soil is high and it is transferred with relative ease to crops. European Food Safety Agency (EFSA) has identified the consumption of chocolate as a source of Cd contamination, and established maximum Cd limits allowed in cacao-based products. Therefore, research related to tolerance and reduction of Cd toxicity in a cacao becomes important. The main objective of the present study was to systematize the main factors that interfere in the uptake of soil Cd by the cacao tree and its strategies for tolerance to toxicity. A systematic review of the literature was conducted using six databases for academic research (Google Academic, PubMed, Springer, CAPES Peri-odical, Scielo and Science Direct). To avoid publication bias, a previously established protocol and inclusion and exclusion criteria were used. The main factors related to the uptake of soil Cd by the cacao tree were soil pH, soil Cd availability, genotype, the geographical location, agronomic factors such as phosphate fertilizersand Cd interaction with other minerals/metal nutrients such as Zn and Mn present in the soil. To reduce the toxicity of this metal, cocoa promotes some tolerance strategies such as uptake and transfer of Cd from the root to the shoot, molecular and biochemical changes, Cd partition between plant organs and Cd sequestration at the cellular level. So, this systematic review contributed to the acquisition of knowledge for the genetic improvement of cacao tree, concerning toxicity tolerance strategies and reduction of soil Cd uptake.

    Analysis of 'Marrakesh limetta' (Citrus x limon var. limetta (Risso) Ollitrault, Curk & R.Krueger) horticultural history and relationships with limes and lemons

    Krueger, RobertRivera, DiegoBermudez, AntonioObon, Concepcion...
    20页
    查看更多>>摘要:Citrus (Rutaceae) species have a relevant role in the culture of the Mediterranean countries, not only for their gastronomic or medicinal value, but especially for their ornamental and symbolic importance. The interest on Citrus diversity led to collect and cultivate numerous cultivars around the Mediterranean Sea, at least since the Roman period. Their image is documented in mosaics, paintings, sculptures and coins since the first millennium BC. Among these, sweet and sour limettas Citrus x limon var. limetta (Risso) Ollitrault, Curk & R.Krueger stand for their history and conservation status. Presently sour limetta is cultivated in Morocco, especially in Marrakesh, and in the Valle de Ricote (Murcia, Spain). Our work has three main objectives: to characterize sour limetta on the basis of Spanish and Moroccan populations, as well as its cultural relevance throughout history, especially in painting, gastronomy, agriculture and gardening, to determine their relationships with limes and lemons and to evaluate the availability and state of conservation of that legacy. We analyze and illustrate the morphology of the plant, flowers and fruits of sour limetta and their differences with other related citrus species and cultivars: sweet limetta, lemon, common lime, Persian lime, bergamot, mellarosa, Meyer lemon, rough lemon, Pursha Romana lime, Palestine sweet lime, Pomum Adami lemon and Rangpur lime. The above species and cultivars were selected on the base of their known ancestry and pomological characters. The distinctive characters are useful for the identification of sour limetta in the field but also for the identifying images of this plant in paintings. We analyzed the ancestry of the different taxa involved and compared the classification based on phenotypes with the classification based on the proportion of ancestors' genomes in each individual taxa. The image of sour limetta appears in different paintings, from the van Eyck's Ghent Polyptych (early 15th century), and numerous still life works by Italian and Spanish authors of the 16th to 18th centuries. A peculiar type of evidence is provided by presence of limetta in the pictorial catalogues of the Medici's fruit collections displayed in a series of paintings by Bartolomeo Bimbi. This distinctly sour limetta is sold in the markets of Rabat (and other cities of Morocco), either fresh, candied or brined and canned. It is an important ingredient of traditional Moroccan cuisine. Although sour lime was a widely used ingredient in Italian cuisine during the 17th century, its state of preservation is deplorable outside of Morocco, and even there it is gradually replaced by lemons. We recommend its cultivation as a resource both for obtaining rootstocks as well as for its fruits, both for Moroccan cuisine and that of other parts of the world for the excellent aromas and flavors that they contribute to the dishes. In this way, by promoting its crop, we will prevent it from disappearing. It is also important to preserve sour lime in germplasm banks.

    Effects of genotype and sodium p-nitrophenolate on microspore embryogenesis and plant regeneration in broccoli (Brassica oleracea L. var. italica)

    Zhao, YingZheng, WenfengLi, JieQi, Xinghua...
    7页
    查看更多>>摘要:Isolated microspore culture has been applied in breeding to obtain doubled haploids (DH) and therefore shorten the time of breeding process; however, the production of pure broccoli lines using DH produced in isolated microspore culture has not been sufficiently developed. This study aimed to establish an effective microspore culture protocol for broccoli for hybrid breeding purposes. Microspores of thirteen broccoli genotypes were successfully induced to form embryos in NLN-13 liquid medium. Three genotypes, BB1, BB4, and BB6, were cultured in NLN-13 medium containing sodium p-nitrophenolate (SPN) ranging from 0 to 0. 2 mg center dot L-1, to improve microspore embryogenesis and plantlet regeneration without callus development. The addition of 0.01-0.2 mg center dot L-1 SPN enhanced the frequency of microspore embryogenesis. Adding 0.1 mg center dot L-1 SPN yielded the maximum number of embryos per bud in BB1, BB4, and BB6, which were 1.92-, 2-, and 2.1-folds higher than the control, respectively. The suitable concentration (0.1 mg center dot L-1) of SPN application could enhance the frequency of direct plantlet regeneration for broccoli. Further, average spontaneous diploidization rate for BB1, BB4, and BB6 was 51.28%. This is the first study to utilize SPN in improving the frequency of microspore embryogenesis and plantlet regeneration, and offer an efficient method for the large production of DH lines that needed in hybrid seeds and genetic study in broccoli.

    Light supplement by dark break shortens the growth period from bulblet to virus-free seed garlic

    Fan, BaoliShang, YuntaoHan, JingyiCao, Baoxin...
    9页
    查看更多>>摘要:The garlic (Allium sativum L.) industry is faced with severe problems such as virus accumulation and low reproduction coefficients due to long-term asexual propagation. In vitro propagation may be an effective method to solve such problems. In the present study, a complete virus-free and rapid propagation technology system from explants to bulblets have been established based on a tissue culture method using the inflorescence of the introduced and domesticated Xinjiang purple peel garlic "Jiangjin No.1 '' as explants. The inflorescences were divided into three groups based on the length ratio of the flower stem to the pseudostem to optimize the harvest time of explants. The result indicated that the inflorescences harvested during the stage when the ratio of flower stem length to pseudostem length was 08-0.9 had the best induction efficiency of bulblets. The obtained virusfree seed garlic and commercial garlic were tested for physio-biochemical and viral indices. Subsequently, the effects of different photoperiod treatments and culture media on the growth of plants originating from the bulblets were assessed. Our results indicated that the virus-free seed garlic and commercial garlic exhibited good virus-free effects and their quality was improved to a large extent. Concomitantly, the breeding efficiency was nearly five times higher than that of traditional breeding methods, resulting in greatly reduced production cost. The formation of underground bulbs was significantly affected by photoperiod treatment, while substrates had a slight influence. The underground bulb formation rate could reach 96% when the bulblets were cultivated on an inorganic substrate under the light source to break the darkness at night. The whole process from bulblets to virus-free seed garlic was completed in a relatively short period (four months) by providing the required nutrients regularly and replenishing artificial light. Compared with conventional field planting, the production period of virus-free seed garlic was shortened by approximately a year. Overall, our study can give important insights into the process optimization of virus-free seed garlic preparation, and make the reproduction, popularization and industrialization of seed garlic free of virus a reality.

    Umbrella review on chilling injuries: Post-harvest issue, cause, and treatment in tomato

    Rai, AnjaliKumari, KhushbooVashistha, Prabhat
    16页
    查看更多>>摘要:Mature tomatoes (Solanum lycopersicum) have a very short shelf life which deteriorates quickly at ambient temperatures. Low temperature storage is the most successful and commonly used treatment to slow down the ripening process and decay development in mature or green tomatoes. However, low temperature may induce a disorder "chilling injury (CI)" which could limit the storage time of tomatoes. This review will summarize the currently published biochemical and genetic knowledge about the potential development of chilling injury (CI) in tomato fruit. It encompasses all studies reported on pre and postharvest issues and treatments that may affect the occurrence and severity of CI. This review paper will provide a better insight to understand the detailed mechanism and genes involved in the process of CI in tomato and help investigate the areas which need to be further explored.

    Insights into the molecular basis of a yellow leaf color mutant (ym) in tomato (Solanum lycopersicum)

    Cheng, MozhenMeng, FanyueMo, FuleiChen, Xiuling...
    13页
    查看更多>>摘要:Leaf color mutants are ideal materials for studying mechanisms of plant such as photosynthesis, photorespiration, the pigment biosynthesis, and some other agronomic traits. Meanwhile, leaf color mutants are widely used in the screening of the offspring of cross breeding. The molecular mechanisms of yellow leaf and REDOX balance of tomatoes have not been explored. Here we obtain a naturally yellow leaf tomato mutant (ym). In order to further understand the role of REDOX, a series of experiments are conducted to assess the physiological and molecular reactions of two tomato lines, ym and zs4. The results showed that the ym mutant cells accumulated excessive H2O2. The chlorophyll content of the mutant decreased greatly. The chloroplast structure of the mutant is observed by transmission electron microscope (TEM). The results showed that mutant's chloroplast membrane structure is seriously damaged. A transcriptomic approach is used to analyze the gene regulate etiolation traits in ym mutant tomato plants. Many genes are enriched in oxidation-reduction (REDOX). Kyoto encyclopedia of genes and genomes (KEGG) revealed that DEGs are involved in pathways such as plant hormone signal transduction, carotenoid biosynthesis, MAPK signaling pathway, phenols and flavonoids synthesis, and pentose and glucuronate interconversions, which further confirmed the role of yellow trait. Overall, the damage of chloroplast structure, low pigment content and low level ROS scavenging capability are the main factors leading to yellow leaf. Main Conclusion Damage of chloroplast structure lead to the low level of the chlorophyll and the REDOX imbalance cause tomato yellow leaf color.

    Multi-walled carbon nanotubes enhanced the antioxidative system and alleviated salt stress in grape seedlings

    Liu, MinYang, XiangliZhang, YiHui, Hongtai...
    8页
    查看更多>>摘要:To enhance the salt resistance of seeds and seedlings for grapes under salt stress, multi-walled carbon nanotubes (MWCNTs) at 30, 60, 90, 150, and 180 mu g/mL, respectively, were used to treat gape seedlings, and the seed germination and physiological characteristics grape seedlings were studied. MWCNTs at 90 mu g/mL exhibited the best results, increased root length and germination rate compared with other concentrations. Moreover, MWCNTs at 90 mu g/mL mostly decreased the content of MDA in leaves and increased the activities of superoxide (SOD), peroxidase (POD), and catalase (CAT). At the same time, it increased the activities of dehydroascorbate reductase (DHAR), ascorbate peroxidase (APX), glutathione-S-transferase (GT), and glutathione reductase (GR). MWCNTs treatment also maintained the scavenging activity of DPPHGreek ano teleia and increased the ratios of GSH:GSSG and AsA:DHA. These results suggested that MWCNTs with the appropriate concentration of 90 mu g/mL could significantly enhance the reducing ability through direct and indirect antioxidant systems (the AsA-GSH cycle) and maintain a high antioxidant capacity of grape seedlings under salt stress.