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Industrial Crops and Products
Elsevier Science Publishers
Industrial Crops and Products

Elsevier Science Publishers

0926-6690

Industrial Crops and Products/Journal Industrial Crops and ProductsSCIISTPEI
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    Preparation, structure and α-glucosidase inhibitory of oligosaccharides by enzymatic hydrolysis from Annona squamosa polysaccharide

    Sun Y.-Y.Sun H.-Q.Pan L.-C.Jia Y.-Q....
    10页
    查看更多>>摘要:? 2022 Elsevier B.V.Annona squamosa (AS) has relatively high content of polysaccharides which had effective hypoglycemic activity. The oligosaccharides have advantages in the digestion and absorption. However, there were few reports on oligosaccharides from AS. In the manuscript, pectinase was utilized to degrade Annona squamosa polysaccharide (ASP). The product was acetylated, purified by silica gel column and deacetylated to obtain a novel oligosaccharide Z. The structure of Z was identified with NMR, mass spectrometry and monosaccharide composition analysis. The results demonstrated that the oligosaccharide Z was a trisaccharide with molecular weight of 518 Da. The structure of oligosaccharide Z was linear skeleton composed of β-D-Glc-(1→2)-β-D-Glc-(1→3)-α-D-GalA. The oligosaccharide Z can effectively inhibit the activity of α-glucosidase with reversible type and mixed-type inhibition. The oligosaccharide Z had effective binding effect with α-glucosidase from molecular docking experiments. The results revealed that the oligosaccharide Z has the potential to be utilized as α-glucosidase inhibitor. The manuscript provided theoretical basis for the new applications of AS in food industry and pharmaceutical industry. It also provided valuable clues for finding new potential hypoglycemic drugs from natural sources.

    Melatonin regulated glucosinolate profile via modulation of genes related with sulfur and nitrogen metabolism in Brassica rapa ssp. pekinensis

    Teng Z.Zheng W.Yu Y.Zhu Z....
    11页
    查看更多>>摘要:? 2022 Elsevier B.V.Melatonin (MT) is a multi–regulatory molecule eliciting pleiotropic effects on plant growth and development. Glucosinolates (GS) are a group of sulfur (S)-rich and nitrogen (N)-containing secondary metabolites that play an important role in plant defense. Although MT treatment is known to strongly induce expression of GS metabolism related genes, it still remains unclear how MT modulates GS profile. In order to better understand the molecular mechanisms of MT-regulated GS profile in Brassica rapa, comparative transcriptomics and HPLC analyses were conducted. Upon MT treatment, overall GS accumulation increased 3.2-fold, along with emergence of three new GS of glucocochlearin (GCR), glucoerucin (GEC) and 4-hydroxyglucobrassicin (4-OHGBC), and aliphatic GS was the most abundant form constituting 63% of the total GS content. Besides, MT treatment generated eleven differentially expressed genes involved in GS biosynthesis, among which eight encode GS pathway enzymes, and three encode MYB transcription factors modulating expression of GS biosynthesis genes. This was accompanied by significantly elevated expression of three S assimilation-related genes and glutamine synthetase gene involved in N assimilation. In vitro disk-diffusion bioassay demonstrated that lyophilized powder of MT-treated rosette leaf, as well as hydrolyzed products of GCR and GEC significantly inhibited the growth of Sclerotinia sclerotiorum. There was a statistically significant negative correlation between 4-OHGBC and colony growth rate, and between 4-methoxyglucobrassicin (4MeGBC) and sclerotia number. Taken together, the results suggested that exogenous MT treatment altered GS profiles and contents by activating the expression of the primary metabolism genes for S and N assimilation, as well as the secondary metabolism genes for GS biosynthesis, thereby playing a prominent role in S. sclerotiorum resistance in B. rapa. MT as an amphiphilic bio-substance, which can easily pass though the cell membrane, may offer a new integrated strategy for ecofriendly and sustainable management to control plant diseases.

    Influence of the mowing and drying on the quality of the peppermint (Mentha x piperita L.) essential oil: Chemical profile, thermal properties, and biological activity

    Radic D.Blagojevic S.Bazarnova J.G.Smyatskaya Y.A....
    9页
    查看更多>>摘要:? 2022 Elsevier B.V.In this study, the influence of the mowing and drying on the chemical profile, thermal behavior, antioxidant activity (DPPH, CUPRAC, FRAP, ABTS, HRSA, and TBARS), microbiological data (Candida albicans (ATCC 10231), Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Candida albicans (ATCC 10231), Bacillus subtilis (ATCC 6633), Proteus vulgaris (ATCC 13315), Proteus mirabilis (ATCC 14153) and Aspergillus niger (ATCC 16404) and cytotoxic activity of peppermint samples (HeLa, LS-174, A549, and MRC-5) was investigated. Chemical profiles showed that mowing did not have a significant impact on essential oil composition compared to drying. Menthone was the principal compound in essential oil from the fresh material, while menthol was the main compound in the samples from the dried ones. These differences influenced the biological activity of the samples, where fresh had better antioxidants, while dried had better antimicrobial activity. Thermal analysis showed that samples completely evaporated to about 120–123 °C in non-isothermal conditions, while in isothermal conditions the time required for complete evaporation was 20–30 min, depending on the sample. An artificial neural network model was developed, for the anticipation of antioxidant activity. These models showed good prediction properties (the r2 value during the training cycle for output variables was 1.000).

    Quantitative trait locus mapping and transcriptome analysis reveal candidate genes for a stem bending mutant in rapeseed (Brassica napus)

    Zhang R.Fan Y.Liu M.Li S....
    10页
    查看更多>>摘要:? 2021 Elsevier B.V.Rapeseed is one of the most important oilseeds crops worldwide. Although stem development greatly affects crop yield, its molecular mechanisms remain elusive in rapeseed. This study found a stem bending 1 (stb1) mutant in rapeseed with abnormal stem development and performed phenotype characterization, genetic analysis, quantitative trait locus mapping, and transcriptome analysis. The key stage of stem bending in stb1 mutant occurred at Biologische Bundesanstalt, Bundessortenamt and CHemical industry (BBCH) stages 59–61, from the end of stem elongation to early flowering. The number of vessels and the degree of development of stem xylem, sclerenchyma, and parenchyma cells were extremely lower in stb1 mutant than in elite cultivar ZS11. Genetic analysis revealed that stb1 is controlled by a single recessive locus located between markers Bn-A01-p2421445 and Bn-A01-p4230829 on chromosome A01. Using RNA-seq, identified 1631 genes that differentially expressed between mutant and normal F2 plants in the shoot apical meristem and stem. The down-regulated genes were mainly overrepresented in auxin transport and coenzyme metabolism processes. Combining data on the auxin concentrations in stem and expression of candidate genes within the mapping interval in the mutant, it was speculated that the phenotype of the stb1 mutant may be caused by defects in polar auxin transport, as two auxin transport-related genes were obviously down-regulated in the mutant. Among them, BnSOS3-INTERACTING PROTEIN3, which encodes a CBL-interacting protein kinase, was considered the most promising candidate gene for further investigation. These results lay a foundation for better understanding the molecular mechanisms of stem development in rapeseed.

    Poly(ε-caprolactone) blended with thermoplastic waxy starch matrix reinforced with cellulose nanocrystals from Macauba (Acrocomia spp.) Rachis

    Galvani F.Mattoso L.H.C.Marconcini J.M.Mantovani G.L....
    12页
    查看更多>>摘要:? 2021 Elsevier B.V.Aiming at a sustainable bionanocomposites, cellulose nanocrystals (CNCs) were extracted by acid hydrolysis from rachis fibers of macauba (Acrocomia spp.) palm trees natural of the Brazilian Pantanal. In a second step, CNCs were incorporated as reinforcement into thermoplastic waxy starch (TPS) phase blended with poly(ε-caprolactone) (PCL), 70/30 (w/w), by higher shear rate co-rotating extrusion. Nanocomposites were formulated with 3.25, 6.5 and 13 wt% CNCs in relation to TPS mass. The nanocomposite containing only 3.25 wt% CNCs improved the mechanical tensile strength by three times and the elastic modulus by more than six times in relation to the TPS/PCL matrix, reaching values close to 12 MPa and 1 GPa, respectively. In addition, the lower amount of CNCs guaranteed the thermal stability of the TPS/PCL nanocomposites (~250 °C), showing an increase in Tonset of 20 °C in relation to the neat matrix. This effect may be associated with the interactions between TPS molecules and CNCs, as observed in the rheological measurements. Therefore, this study showed the potential of macauba as a renewable source for CNC extraction, bringing higher economic value to this plant and establishing its sustainable productive chain.

    Next-generation high-performance sustainable hybrid composite materials from silica-rich granite waste particulates and jute textile fibres in epoxy resin

    Chaturvedi R.Pappu A.Tyagi P.Patidar R....
    11页
    查看更多>>摘要:? 2022 Elsevier B.V.In recent years, extensive efforts have been made to prepare composite materials using renewable and sustainable raw materials. Albeit significant advancements in bio-based fibre-reinforced composites, high water absorption and eventual degradation in properties remain a persistent issue the needs to be addressed. In this study, the epoxy-based hybrid green composites were prepared, where granite waste particulates were used as filler in synergy with jute fibre reinforcement. Milled granite particulates were characterized for their physical, chemical, mineralogical, and morphological aspects. Composites were prepared using hand layup followed by compression moulding techniques with high filler percentages (30, 50, and 60 wt%) and jute fibre content (2 and 4 layers). The influence of filler and fibres on physical properties (water absorption, thickness swelling, and density), mechanical properties (tensile, flexural, and impact), electrical resistivity, and thermal conductivity of the prepared composites were studied. Loading with 60 wt% of granite waste filler and 4 layers of jute fibres resulted in composites with around 5% higher flexural strength, 16% higher tensile strength, 148% higher impact strength, and almost 150% higher thermal conductivity as compared to neat epoxy. The composites also exhibited very low water absorption (0.1%) with a nominal increase in density while containing electrical insulation property despite decreasing volume resistivity. Due to the high amount of mineral waste and natural fibre usage, these materials have very low embodied energy content, thus making them cost-effective, sustainable, and environment-friendly materials for structural and architectural applications. Additionally, the study also provides a potentially viable route for the effective utilization of mineral wastes, such as granite, whose growing accumulation has serious ramifications for the environment, living organisms, and the economy.

    Linking Phyllostachys edulis (moso bamboo) growth with soil nitrogen cycling and microbial community of plant-soil system: Effects of plant age and niche differentiation

    Zhang M.Wang W.Bai S.H.Xu Z....
    13页
    查看更多>>摘要:? 2022 Elsevier B.V.Understanding the impacts of plant age on bamboo growth, soil nutrient and functional microorganism can assist in the development of management practices to maximize the benefits of nutrients and functional endophytic and soil microbes in moso bamboo plantations. In this study, moso bamboos of different ages (0.5, 2.5, 4.5 and 6.5 years) were selected. The above-ground biomass, soil properties and endophytic microbes of above-ground tissue and stump root of different age bamboos were quantified, and their interactive relationships were also examined. There were negligible differences in the heights and above-ground fresh biomass among the bamboo plants of different ages, but net nitrogen (N) mineralization and potential nitrification rates of soils were significantly affected by the bamboo ages. The soil nprA gene abundances decreased with the bamboo ages, and soil AOB amoA gene abundances of the 0.5-yr bamboo were significantly higher than those of the other older plants. Average soil urease activities of the 2.5-yr, 4.5-yr and 6.5-yr bamboos were 81.4%, 81.1% and 88.2% lower than those of the 0.5-yr bamboo, respectively. Bacterial diversity indices and richness estimators in the soils were significantly higher than those in the above-ground tissues. Endophytic microbial community structures were more sensitive to the bamboo ages than the soil counterparts, and endophytic microbial community diversities and structures in the above-ground tissues were significantly changed with the bamboo ages. Bacterial and fungal community structures in the above-ground tissues were significantly different from those in the soils. Bamboo ages significantly affected the N transformations in the soils and endophytic community structures, but niche differentiations among the above-ground tissue, stump root and soil outweighed the plant ages in shaping the whole microbial communities of plant-soil system.

    The effect of harvest strategy on the energy potential of Jerusalem artichoke

    Bogucka B.Jankowski K.J.
    11页
    查看更多>>摘要:? 2021 The AuthorsJerusalem artichoke (Helianthus tuberosus L.) is a crop with a high potential for energy generation on account of its broad range of industrial applications, high yield potential and relatively low climatic and agronomic requirements. The aim of this study was to determine the effect of different harvest strategies on the energy potential of Jerusalem artichoke (JA) aerial biomass. The yield and energy efficiency (EE) ratio of JA biomass harvested (i) once (August) and (ii) twice (June and October) during the growing season were investigated in a three-year field experiment. The study was conducted in north-eastern Poland. The energy inputs (EI) in JA production were determined at 23.0–23.5 GJ ha?1 in the year of plantation establishment and at 12.1–14.6 GJ ha?1 y?1 in the second and third year. Energy inputs were 11–12% higher when JA biomass was harvested twice during the growing season. Dry matter yield (DMY) was highest in the second year (28.1 Mg ha-1), and it was 32% and 58% lower in the first and third year of the study, respectively. Dry matter yield was considerably higher when JA biomass was harvested once in August. The energy gain (EG) in JA production ranged from 144 (year 3) to 346 GJ ha?1 y?1 (year 2). The EE ratio of JA biomass production ranged from 11.3 and 12.3 (years 1 and 3) to 26.1 (year 2). Energy gain and the EE ratio were significantly higher (by 67% and 63% on average, respectively) in treatments with a single- than a double-cut harvest strategy. The study demonstrated that both productivity and the volume of energy accumulated in JA biomass can be optimized by selecting an appropriate harvest strategy (single-cut or double-cut harvest during the growing season).

    Bio-chemical characterization and in silico computational experimental properties of Trianthema triquetra Rottler & Willd.: A desert medicinal plant for industrial products

    Ahmad S.Aziz M.Saleem H.Wazir M.A....
    9页
    查看更多>>摘要:? 2022 Elsevier B.V.This research work prospects the phytochemical and biological properties of different polarity solvent (methanol, ethyl-acetate, n-hexane, and n-butanol) extracts of Trianthema triquetra Rottler & Willd. The assessment of chemical profile was done by ascertaining total bioactive contents and UHPLC-MS analysis. Similarly, biological profile was analyzed via determination of antioxidant (DPPH, ABTS, FRAP, CUPRAC, phosphomolybdenum and metal chelation) and key enzyme inhibition (acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase) potential. Principal component analysis (PCA) was also conducted to highlight the correlation amongst bioactive contents and tested bioassays. The highest proportion of total phenolic (59.85 mg GAE/g extract) and flavonoid (19.85 mg RE/g extract) contents were exhibited by methanol extract which might be correlated with its higher antioxidant capacity. Similarly, UHPLC-MS phytochemical profiling of the methanol extract unveiled the identification of 26 secondary metabolites belonging to phenolic, flavonoids, glucoside, coumarin, alkaloid, and fatty acid derivatives. The ethyl-acetate fraction exhibited the highest inhibition against BChE, α-amylase and α-glucosidase, while the n-butanol and n-hexane extracts were found to be the active against AChE and tyrosinase enzymes and the molecular docking studies explained the possible mechanism of enzyme inhibition. Results of antioxidant assays were further confirmed by PCA analysis, which shows highly effective association amid total bioactive contents and antioxidant assays.

    Comparative analysis of drought-responsive physiological and transcriptome in broomcorn millet (Panicum miliaceum L.) genotypes with contrasting drought tolerance

    Yuan Y.Liu L.Gao Y.Yang Q....
    18页
    查看更多>>摘要:? 2022 Elsevier B.V.Climate change has caused severe drought, affecting global crop production. Broomcorn millet is a drought-tolerant crop preferred for water-saving agriculture because of its short life cycle and high water use efficiency. This study evaluated the drought tolerance of 300 broomcorn millet varieties from 21 sources under well-watered, semi-arid conditions (Yulin, Shaanxi, China) and unwatered, arid conditions (Dunhuang, Gansu, China). Two broomcorn millet varieties with contrasting drought tolerance attributes, DT 43 (drought-tolerant) and DS 190 (drought-sensitive), were selected for comparative physiological and transcriptional assessment under the two drought stress conditions (polyethylene glycol 6000 [PEG-6000] and soil drought) and corresponding melatonin treatments. The two forms of drought stress decreased photosynthetic capacity and triggered transcriptome reprogramming in both broomcorn millet cultivars. However, PEG induced a more ‘severe’ and ‘rapid’ drought stress than the ‘milder’ and ‘slower’ soil moisture drought stress. Moreover, PEG stress caused severe growth arrest and photosynthesis inhibition, especially for DS190. About 61.38% and 48.78% differentially expressed genes (DEGs) were up-regulated in DT 43 under PEG and soil drought stresses, respectively. Moreover, 74.31% and 54.59% DEGs were up-regulated in DS 190 under PEG and soil drought stresses, respectively. Most DEGs in DT 43 were significantly enriched in hormone signal transduction, mitogen-activated protein kinase (MAPK) signaling, and carbon metabolism pathways. However, most DEGs in DS 190 were enriched in plant photosynthesis, chlorophyll metabolism, and nitrogen metabolism pathways. Moreover, melatonin enhanced the drought resistance of the two genotypes, increasing photosynthetic and antioxidant enzyme activity and thus mitigating transcription response. Therefore, these unique mechanisms of enhancing drought resistance can improve bioenergy crops, especially for the cultivation of drought-tolerant varieties.