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Talanta
Pergamon Press
Talanta

Pergamon Press

0039-9140

Talanta/Journal TalantaSCIISTPEIAHCI
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    Mitigating hook effect in one-step quantitative sandwich lateral flow assay by timed conjugate release

    He, GuozhenDong, TaoYang, ZhaochuJiang, Zhuangde...
    7页
    查看更多>>摘要：Sandwich lateral flow assay (LFA) is one of the most successfully commercialized paper-based biosensors, which offers a rapid, low-cost, one-step assay. Despite its advantages, conventional sandwich LFA is fundamentally limited by the high-dose "hook" effect-a phenomenon that occurs at very high analyte concentrations and results in false-negative results. In this paper, we present a novel strategy of automatic timed detection antibody release to mitigate the hook effect in sandwich LFA without additional manual steps. We introduced an intermediate pad treated with saturated sucrose solution to regulate the flow between the nitrocellulose membrane and the conjugate pad in order to delay the reaction between detection antibodies and analytes. Using C-reactive protein (CRP) as a representative analyte, we demonstrated that our strategy exhibited a range of detection 10 times wider than that of our conventional LFA, without sacrificing the limit of detection. Comparing to other published strategies, our work could offer a one-step, cost-effective approach that is closely unified with the benefits of the LFA.
      原文链接:
    • NSTL
    • Elsevier

    A high-throughput and cost-effective microplate reader method for measuring persulfates (peroxydisulfate and peroxymonosulfate)

    Kalogerakis, Georgina C.Boparai, Hardiljeet K.Yang, Minqing IvySleep, Brent E....
    12页
    查看更多>>摘要：Frequent use of persulfates as oxidants, for in situ chemical oxidation and advanced oxidation processes, warrants the need for developing a fast and efficient method for measuring persulfate concentrations in aqueous samples in the lab and on site. Here, we propose a modified method, based on Liang et al.'s (2008) spectrophotometric method, for measuring both peroxydisulfate (PDS) and peroxymonosulfate (PMS) in the aqueous samples. Our method involves a deep 96-well plate, multi-channel pipettes, a small orbital shaker, and a microplate reader; allowing the preparation and analysis of up to 96 samples in one run. Our proposed method shortens the time by 10 folds, consumes only-2% of the original reagents, and generates only-2% of the liquid waste compared to the Liang et al.'s method, thus, making our method high-throughput, time-efficient, and cost-effective with reduced environmental impact. The presented microplate reader method is validated in terms of linearity, LOD, LOQ, accuracy, precision, robustness, and selectivity. All the parameters satisfied the acceptance criteria, according to ICH guidelines. The linearity of calibration curves was evaluated by performing the F-test. In general, our method has linear ranges from 20 to 42,000 and 5 to 40,960 mu M for PDS and PMS, respectively. Accuracy (% recovery) results suggested that the LOD and LOQ based on the standard deviation of y-intercepts of the regression lines were the most reliable. The LOD/LOQ values for PDS and PMS were 14.7/44.1 and 4.6/14.4 mu M, respectively. The proposed method was also modified to work with a standard cuvette spectrophotometer and was validated. A comparison with the UHPLC analysis of PDS showed that our microplate reader method performed equivalently or even outperformed the UHPLC method, in the presence of common groundwater constituents and organic contaminants.
      原文链接:
    • NSTL
    • Elsevier

    A peroxidase-like nanoenzyme based on strontium(II)-ion-exchanged Prussian blue analogue derivative SrCoO3/Co3O4 nanospheres and carbon quantum dots for the colorimetric detection of tigecycline in river water

    Duan, DingFang, XiaoyuLi, Kang
    9页
    查看更多>>摘要：In this work, for the first time, we derived a composite of perovskite oxide SrCoO3 and Co3O4 by annealing the Prussian blue analogue exchanged with strontium ions and modified with carbon quantum dots (CQDs). Its peroxidase-like catalytic activity was explored. The peroxidase-like activity was mainly evaluated by the rate of the chromogenic reaction. When H2O2 was present in the reaction system, the colorless substrate 3,3 ',5,5 '-tetramethylbenzidine (TMB) was oxidized into a blue product (oxTMB), and this was monitored by UV-vis absorption spectrum. Among them, the SrCoO3 and CQDs not only promoted the generation of superoxide anion radicals (O2- center dot) and electron-hole pairs (h+) in the reaction system, but also accelerated the electron transfer between the substrate TMB and H2O2. Therefore, the peroxidase-like catalytic activity of the reaction system was significantly improved. Moreover, the complexation of tigecycline (TGC) and CQDs@SrCoO3/Co3O4 composite enhanced the peroxidase-like catalytic activity of the reaction system. Based on this, using TGC and TMB as template molecules, a molecularly imprinted colorimetric sensor for detecting TGC was constructed. The absorbance difference of the reaction system was linear with the TGC concentration in the range of 0.02-6.0 mu M, and the detection limit was 4.46 nM. Furthermore, the proposed sensor had high selectivity and applied to the detection of TGC in Pearl River water.
      原文链接:
    • NSTL
    • Elsevier

    Biosensors for Caspase-3: From chemical methodologies to biomedical applications

    Lei, QianHuang, XueyanZheng, LijuanZheng, Fan...
    24页
    查看更多>>摘要：Caspase-3 plays irreplaceable roles in apoptosis and related diseases. An imbalance in the measured levels of Caspase-3 is implicated in irreversible apoptosis. Therefore, the detection of Caspase-3 is of great significance for apoptosis imaging and the evaluation effect of early tumor treatment and other diseases. Herein, advances in the recent innovations of Caspase-3 response fluorescence biosensors, including molecular probes and nanoprobes, are systematically summarized in sections corresponding. The performances of various luminescence probes in Caspase-3 detection are discussed intensively in the design strategy of chemical structure, response mechanism and biological application. Finally, the current challenges and prospects of the design of new Caspase-3 responsive fluorescence probes for apoptosis imaging, or similar molecular event are proposed.
      原文链接:
    • NSTL
    • Elsevier

    Selective and sensitive detection of miRNA-198 using single polymeric microfiber waveguide platform with heterogeneous CHA amplification strategy

    Yu, YueZou, GangFeng, XiaohuiYang, Kexin...
    6页
    查看更多>>摘要：Pancreatic cancer (PC), which has a high fatality rate, is a kind of cancer with poor diagnosis and poor prognosis. Development of selective and sensitive detection platform to diagnose and prognostic of PC has attracted considerable attention. The miRNA-198 has been reported a potential prognostic and early diagnostic marker signature of PC. Herein, we report a novel sensitive detection of miRNA-198 in buffer and serum based on one dimensional chitosan/fluorescein isothiocyanate (CS/FITC) fluorescent microfiber waveguide system combined with the catalytic hairpin assembly amplification strategy. By combination with condensing enrichment effect, the proposed detection platform exhibited high specificity and sensitivity to miRNA-198 target, giving a detection limit as low as 2 fM. More importantly, the proposed detection platform can be applied directly to distinguish the expression of miRNA-198 in clinical serum, affording the ability to distinguish pancreatic cancer patients from those of healthy human beings, and quantify the expression variation of miRNA-198 for the pancreatic cancer patients before and after resection, which may pave the way to develop novel clinical diag-nostic equipment for cancer diagnosis and therapeutic evaluation.
      原文链接:
    • NSTL
    • Elsevier

    Discrimination of white automotive paint samples using ATR-FTIR and PLS-DA for forensic purposes

    Gomes, Juliano de AndradeDuarte, Juliana MeloSales, Nadia Gabrielle SilvaBraga, Jez Willian Batista...
    12页
    查看更多>>摘要：The consequences of a hit-and-run car crash are significant and may include serious injuries to the victims, health system overload and even victim's death. The vehicle and driver identification are often challenging for local law enforcement. The aim of this study was to develop a methodology to discriminate between automotive paint samples according to the make of the vehicle and its color shade. 143 white samples (collected at traffic accident scenes) were analyzed in situ by Fourier transform infrared spectroscopy with attenuated total reflectance (ATRFTIR) and coupled microscopy. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed for data analysis. The samples were split into three groups: calibration set, validation set and external test set. The figures of merit were calculated to assess the quality of the model. Sensitivity, specificity, and efficiency rates were, respectively, 98,9%, 98.4% and 98.6%, for the calibration set. For the validation group, the classification accuracy was 100%. Correct classification rates for the internal validation set and external test set were 100% and 79.1% respectively. The technique is clean, fast, relatively low-cost, and non-destructive. Damaged regions of the samples were avoided by using the attached microscope. Limiting the age of the samples to a maximum of 10 years was enough to avoid misclassifications due to the natural degradation and weathering of the sample. Since the external test group is formed by underrepresented classes, its correct classification rate (79.1%) can be potentially improved at any time, by including and analyzing more samples.
      原文链接:
    • NSTL
    • Elsevier

    Non-biological fluorescent chemosensors for pesticides detection

    Yang, JiaoChen, Shu-WeiZhang, BingwenTu, Qin...
    13页
    查看更多>>摘要：The ongoing poisoning of agricultural products has pushed the security problem to become an important issue. Among them, exceeding the standard rate of pesticide residues is the main factor influencing the quality and security of agricultural products. Moreover, the abuse of pesticides has introduced a large amount of residues in soil and drinking water, which will enter the food chain to the human body, leading to neurological disorders and cancer. Therefore, great efforts have been devoted to developing fluorescent sensors for detecting pesticide in a facile, quickly, sensitive, selective, accurate manner, which exhibit greater advantages than some traditional methods. In this review, we mainly focus on summarizing the non-biological fluorescent probes for organic pesticides detection with the detection limit of micromole to nanomole, including organic functional small molecules, calixarenes and pillararenes, metal organic framework systems, and nanomaterials. Meanwhile, we described the different sensing mechanisms for pesticides detection of these mentioned fluorescent sensors, the detection limit of each pesticide, the application in detecting actual samples, as well as their respective advantages and development prospects associated with present non-biological fluorescent sensors.
      原文链接:
    • NSTL
    • Elsevier

    Nanostructure stable hydrophilic hierarchical porous metal-organic frameworks for highly efficient enrichment of glycopeptides

    Zhu, TianyiGu, QinyingLiu, QiannanZou, Xia...
    9页
    查看更多>>摘要：Protein glycosylation plays a vital role in many physiological activities in organisms. Due to the low abundance of glycopeptides and the interference of numerous non-glycopeptides in biological samples, selective enrichment of glycopeptides is of great significance for their successful identification. Metal organic frameworks (MOFs) materials are appropriate for glycopeptides enrichment by virtue of their large specific surface area and outstanding hydrophilic properties. However, the instability of hydrophilic MOFs in acidic solutions have severely limited their applications. In this work, a rational facile strategy was established to synthesize a stable hydrophilic hierarchical porous MOF (denoted as HP-MOF-Arg@mSiO(2)). This new material improved the selectivity and sensitivity of enrichment for glycopeptides via modification of arginine groups. More importantly, the mesoporous silica layer was introduced to enhance the stability of MOFs in aqueous solution and achieve the size exclusion effect of large-size proteins in complex samples. Overall, owing to the unique hierarchical porous and the hydrophilic modification, the synthesized HP-MOF-Arg@mSiO(2) materials showed excellent hydrophilicity and hydrolytic stability, resulting in outstanding specific separation capacity in glycopeptides enrichment. A total of 521 and 342 glycopeptides were respectively captured from 2 mu L human serum digests and mouse testis tissue digests, revealing the potential of the materials in the study of glycoproteomics in complex biological samples.
      原文链接:
    • NSTL
    • Elsevier

    Diffusive Gradients in Thin-films technique for uranium monitoring along a salinity gradient: A comparative study on the performance of Chelex-100, Dow-PIWBA, Diphonix, and Lewatit FO 36 resin gels in the Scheldt estuary

    Smolikova, VendulaPelcova, PavlinaRidoskova, AndreaLeermakers, Martine...
    10页
    查看更多>>摘要：Monitoring of uranium in the environment using the Diffusive Gradients in Thin-films (DGT) technique gains in importance as it can provide unique information about the bioavailability of the element and allows its long-term in-situ measurement. Hence, in this study, four DGT binding phases (Chelex-100, Dow-PIWBA, Diphonix, and Lewatit FO 36 resins) were evaluated for uranium monitoring to assess the robustness of their performance in estuarine and marine environments. These DGTs were deployed along the Scheldt estuary (Belgium and the Netherlands) over four campaigns between 2014 and 2021. The DGT performance (ratio of the DGT-determined vs. dissolved U concentration in grab water sample) varied with the water salinity. The Chelex-100 DGTs generally provided good performance in freshwater (median ratios close to 1.0), but an inverse correlation with the increasing salinity was observed (median ratios 0.7 at the stations with salinity >5). The Lewatit FO 36 DGTs provided good performance in the salinity range 0-18 (median ratios 1.0). However, a strong negative influence was observed at stations with high salinity levels (>18, ratio 0.6) and during the long-term deployment in seawater (ratios <0.5 over deployment periods >= 2 days). The Dow-PIWBA and Diphonix DGTs provided overall similar results with excellent performances along the whole salinity gradient (median ratios 1.1 and 1.0, respectively). Nevertheless, the long-term deployment trial in seawater (salinity similar to 27) revealed the robustness of Diphonix DGTs that provided outstanding results even after 28 days of deployment (ratio 1.0). The differences in the performance of tested DGT resins were mostly given by the changes of U speciation along the salinity gradient. The speciation modelling of U showed that calcium uranyl carbonate complexes dominate along the Scheldt estuary (from 97 to 86% seawards) with increasing fraction of UO2(CO3)(3)(4-) (from 2 to 14%) towards the mouth.
      原文链接:
    • NSTL
    • Elsevier

    Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection

    Wang, ChunhuaChu, YujinHan, YingkuanGao, Yakun...
    7页
    查看更多>>摘要：The current outbreaking of the coronavirus SARS-CoV-2 pandemic threatens global health and has caused serious concern. Currently there is no specific drug against SARS-CoV-2, therefore, a fast and accurate diagnosis method is an urgent need for the diagnosis, timely treatment and infection control of COVID-19 pandemic. In this work, we developed a field effect transistor (FET) biosensor based on graphene oxide-graphene (GO/Gr) van der Waals heterostructure for selective and ultrasensitive SARS-CoV-2 proteins detection. The GO/Gr van der Waals heterostructure was in-situ formed in the microfluidic channel through 7C-7C stacking. The developed biosensor is capable of SARS-CoV-2 proteins detection within 20 min in the large dynamic range from 10 fg/mL to 100 pg/mL with the limit of detection of as low as -8 fg/mL, which shows -3 x sensitivity enhancement compared with GrFET biosensor. The performance enhancement mechanism was studied based on the transistor-based biosensing theory and experimental results, which is mainly attributed to the enhanced SARS-CoV-2 capture antibody immobilization density due to the introduction of the GO layer on the graphene surface. The spiked SARS-CoV-2 protein samples in throat swab buffer solution were tested to confirm the practical application of the biosensor for SARS-CoV-2 proteins detection. The results indicated that the developed GO/Gr van der Waals heterostructure FET biosensor has strong selectivity and high sensitivity, providing a potential method for SARS-CoV-2 fast and accurate detection.
      原文链接:
    • NSTL
    • Elsevier