查看更多>>摘要:By a News Reporter-Staff News Editor at Robotics & Machine Learning Daily News Daily News-New research on Uterine Diseases and C onditions - Endometriosis is the subject of a report. According to news reportin g out of Changsha, People's Republic of China, by NewsRx editors, research state d, "Endometriosis is characterized by the ectopic proliferation of endometrial c ells, posing considerable diagnostic and therapeutic challenges. Our study inves tigates AGPAT4's involvement in endometriosis pathogenesis, aiming to unveil new therapeutic targets." Financial support for this research came from Natural Science Foundation of Huna n Province. Our news journalists obtained a quote from the research from Central South Unive rsity, "Our investigation by analyzing eQTL data from GWAS for preliminary scree ning. Subsequently, within the GEO dataset, we utilized four machine learning al gorithms to precisely identify risk-associated genes. Gene validity was confirme d through five Mendelian Randomization methods. AGPAT4 expression was measured b y Single-Cell Analysis, ELISA and immunohistochemistry. We investigated AGPAT4's effect on endometrial stromal cells using RNA interference, assessing cell prol iferation, invasion, and migration with CCK8, wound-healing, and transwell assay s. Protein expression was analyzed by western blot, and AGPAT4 interactions were explored using AutoDock. Our investigation identified 11 genes associated with endometriosis risk, with AGPAT4 and COMT emerging as pivotal biomarkers through machine learning analysis. AGPAT4 exhibited significant upregulation in both ect opic tissues and serum samples from patients with endometriosis. Reduced express ion of AGPAT4 was observed to detrimentally impact the proliferation, invasion, and migration capabilities of endometrial stromal cells, concomitant with dimini shed expression of key signaling molecules such as Wnt3a, b-Catenin, MMP-9, and SNAI2."