查看更多>>摘要：The aim of this study was to increase the Coenzyme Q_(10) (CoQ_(10)) yield from Rhodobacter sphaeroides via genetic engineering pathway. CoQ_(10) plays important roles in many biological processes and has been proven to be effective in the treatment of many diseases. In the present study, the ubiG gene located in CoQ_(10) biosynthesis pathway was effectively overexpressed in Rb. sphaeroides to increase CoQ_(10) production. The growth of host cells was slightly influenced by overexpressing ubiG. The crude CoQ_(10) production was enhanced by 58.31% compared to that from the control. The ubiG mRNA level was significantly increased compared to the wild type harboring empty vector as measured by qRT-PCR. Moreover, the crude CoQ_(10) exhibited strong anti-oxidant activity as measured in vivo by zone of inhibition assay.
查看更多>>摘要：The present study aims at determining the length-weight relationship, sex ratio, relative weight, gonadosomatic indices and length at 50% maturation for the Indian mackerel, Rastrelliger kanagurta (Cuvier, 1817). A total of 791 specimens were collected from Salalah in the Dhofar region during the years 2007 to 2009. For both sexes, a common length-weight relationship was derived from the analysis, that is W = 0.00001×L~(3.4). It was found that the Omani Indian mackerel spawned during August to November and the lengths at 50% maturation were 271 mm for the combined sexes, 267.5 mm for the females and 277.5 mm for the males. The relative condition weight was positively proportion to the gonadosomatic index. The mean sex ratio for the entire fish sample of 0.56±0.1 was significantly different from the anticipated theoretical sex ratio of 0.5 (X~2 = 8.77; df = 1; p＜0.05).
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