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东北农业大学学报(英文版)
东北农业大学学报(英文版)

李庆章

季刊

1006-8104

xuebaoenglish@neau.edu.cn

0451-55191241,55190553

150030

哈尔滨市香坊区木林街

东北农业大学学报(英文版)/Journal Journal of Northeast Agricultural University(English Edition)
查看更多>>本刊主要刊登农学、园艺、植保、资环、动物科学、动物医学、农业工程、农业水利、生物工程、食品科学、基础学科等方面的科研学术论文,及国内外有关学科符合本刊宗旨及办刊内容的论文。本刊已在科技期刊界享有较高的声誉和地位,曾先后多次荣获国家和省级的“优秀科技期刊”奖。
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    Physiological Analysis of Drought Resistance of Seven Cultivars Spring Wheat in Northern Regions of China

    Gao FengmeiZhang FuzhiZhao YuanlingLi Tie...
    1-12页
    查看更多>>摘要:In order to determine the physiological mechanism of drought resistance of northern wheat in China, six drought resistant wheat and one sensitivity to drought wheat were planted in pots. They were subjected to drought treatment and normal water when the plants grew to the 3-leaf stage. Samples were collected at 10, 20, 30, and 40 days after the drought treatment, respectively. The electrical conductivity, photosynthetic parameters, chlorophyll fluorescence parameters, sugar content, proline content, protein content, and active oxygen scavenging enzyme activity of the plants were detected, and the agronomic traits of the wheat varieties were investigated at maturity. The results indicated that the phenotype and yield-related factors of Darkhan 144 changed little under the drought stress. The relative electrical conductivity of Kefeng 6 and Darkhan 166 was lower under the drought stress, and their cell membrane was less damaged. The Darkhan 144 and Darkhan 166 had higher drought resistance coefficients, and were the wheat varieties with stronger drought resistance. However, the physiological mechanisms of drought resistance of these three wheat were different: Darkhan 144 maintained a higher photosynthetic activity under the drought stress; Darkhan 166 maintained a higher protein content, photosynthetic activity and active oxygen scavenging enzyme activity. In addition, other drought-resistant varieties Kefeng 6, Kefeng 10 and Longmai 26 had a higher content of osmoregulatory substances under the drought stress.

    Effects of Biostimulant NEAU10 on Growth of Rice Seedlings and Soil Physicochemical Parameters

    Ding WeiPang YingjieCheng Zhuo
    13-23页
    查看更多>>摘要:The process of rice (Oryza sativa L.) seedling cultivation is often subjected to adverse environmental stress. Biostimulants regulate the robust growth of rice seedlings and play a crucial role in promoting the green and ecological development of agriculture. In this study, 1.0 and 2.0 g • m-2 of the biostimulant were applied to soil in rice seedbeds. Growth indicators of rice, antioxidant enzyme activities and soil physicochemical characteristics were assessed at the 2.5-leaf and 4-leaf stages of rice. The results indicated that applying 2.0 g • m-2 of the biostimulant at both the 2.5-leaf and 4-leaf stages had the most significant promoting effect on rice growth. At the 2.5-leaf and 4-leaf stages, the number of fibrous roots increased by 23.43% and 22.25%, stem base width increased by 19.05% and 19.58%, above ground dry weight increased by 18.09% and 16.47%, root dry weight increased by 19.67% and 18.28%, leaf peroxidase (POD) activity increased by 34.44% and 42.94%, superoxide dismutase (SOD) activity increased by 37.24% and 56.79%, malondialdehyde (MDA) content decreased by 18.60% and 27.67%, and chlorophyll content increased significantly by 28.31% and 34.24%, respectively. At the 4-leaf stage of rice, urease, phosphatase and cellulase activities in the seedbed soil increased by 42.13%, 25.96% and 33.59%, respectively, while soil alkaline nitrogen, available phosphorus and available potassium content decreased by 19.76%, 19.02% and 17.88%, respectively. The application of biostimulants played a crucial role in promoting the growth of rice seedlings and enhancing soil nutrient absorption.

    Polyploidy Induction and Morphological Characteristics in the Asiatic Hybrid Lily (Lilium spp.cultivar dazzling)

    Xiang YixunChen MingxuanChen JianguoYu Zhenyu...
    24-33页
    查看更多>>摘要:The Asiatic hybrid lily cultivar Lilium dazzling (family Liliaceae) is a perennial herbaceous bulbous plant with bright yellow flowers of ornamental values. It also shows resistance to diseases and abiotic stress, making it an ideal parent for breeding studies. This study established a sterile culture system using scales of dazzling lily as explants to induce polyploidy. Adventitious buds growing to 1 cm were treated with different concentrations of colchicine (125, 250 and 500 μmol·L-1), pendimethalin or trifluralin (100, 200 and 300 μmol·L-1, respectively) for 12, 24 and 36 h. Flow cytometry and chromosome counting were used to identify ploidy, and the phenotype of the polyploids was examined by stomatal observation and leaf index determination. With increasing mutagen concentration and exposure time, the mortality and mutagenic rate increased. The optimal treatment group was: 300 μmol·L-1 trifluralin for 12 h, which resulted in 15.55% mortality rate and 42.22% induction rate. The polyploid plants showed a significant increase in stomata length, leaf length and leaf width, with a decrease in stomatal density compared with the control plants. This study provided a basis for polyploid breeding.

    Strategies and Economic Benefit Analysis for Productive Landscape of Rape Flower Sea in Cold Regions

    Zhang JiaxinWu ZhihengZhu XuanboPan Shengkai...
    34-42页
    查看更多>>摘要:The cold regions are located in high latitudes and cold climates. The local excellent ornamental plant resources are relatively scarce. The plant species that are suitable for both ornamental and productive benefits of landscape of flower sea construction are also even fewer. Therefore, it is imperative to introduce and screen the plant resources suitable for cold regions to create the landscape of flower sea. The rape, an oilseed crop, was used as a research object in order to create a productive flower landscape with both ornamental and economic values in cold regions. Four rape flower varieties, Qingza No. 5, 7, 9, and 11, were introduced from Qinghai Hufeng Agricultural Science and Technology Group Co., Ltd. They were planted in the experimental practice base of Northeast Agricultural University in three batches. Development characteristics and seed yield of rape flowers on different sowing dates were studied. The fuzzy probability method was used to comprehensively evaluate the varieties. The results showed that the rape flowers grew well in Harbin City during the experimental sowing period, which could form a good landscape of flower sea and had a considerable rapeseed yield. It could be widely used in cold urban and rural areas, such as Harbin City. In view of the experimental results, the strategies of creating a productive landscape of rape flower sea were proposed and the economic benefits were analyzed. It could change the status quo of a uniform landscape of flower sea in cold regions, help the development of rural tourism, and promote local economic income.

    Effects of Silicon Formulations on Cold Tolerance of Rice Seedlings

    Ren HongyuWang WenboLi XuecongLi Shuai...
    43-52页
    查看更多>>摘要:To investigate the effects of silicon formulations on the cold tolerance of rice seedlings, Song Japonica 16 (not cold tolerant) and Dongnong 427 (cold tolerant) rice varieties were used as test materials and four different types of silicon formulations, Si-50-G, Si-60-G, Si-T-G, and Si-E-G, were applied as foliar sprays at the seedling stage, and a control group CK (equal amount of distilled water) was set up. One week after the first silicon spray, two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12 ℃/10 ℃ for 2, 4, 6, and 8 days. The effects of different silicon formulations on the chlorophyll, proline (Pro) and soluble sugar contents as well as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice seedlings. The results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves, with Si-50-G being the most effective, with a significant increment of 40.17% compared to the CK at 2 days of low temperature. Four silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 days. For Song Japonica 16, the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2, 4 and 8 days of temperature stress, with 73.58%, 20.95% and 217.24% increases compared to the CK, respectively. For 4 and 6 days of temperature stress, the most significant increase in CAT activity was observed in Si-E-G treatment, with 25.70% and 75.78% increases compared to the CK, respectively. For Dongnong 427, the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress, with significant increases of 58.15% and 82.76% compared to the CK, respectively, and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature stress, with significant increases of 97.75% and 245.10% compared to the CK, respectively. It showed that the spraying of silicon formulations could significantly enhance the cold tolerance of rice. This study provided a scientific basis for the rational use of silicon formulations to enhance cold tolerance in rice and had important theoretical and practical significance for ensuring sustainable high and stable rice yields in Heilongjiang Province, as well as for the development of silicon fertilizers.

    A Study on Reconstruction of Surface Wind Speed in China Due to Various Climate Variabilities

    Li YancongLi XichenSun YankunXu Jinhua...
    53-65页
    查看更多>>摘要:Using European Centre for Medium-Range Weather Forecasts Reanalysis V5 (ERA5) reanalysis data, this study investigated the reconstruction effects of various climate variabilities on surface wind speed in China from 1979 to 2022. The results indicated that the reconstructed annual mean wind speed and the standard deviation of the annual mean wind speed, utilizing various climate variability indices, exhibited similar spatial modes to the reanalysis data, with spatial correlation coefficients of 0.99 and 0.94, respectively. In the reconstruction of six major wind power installed capacity provinces/autonomous regions in China, the effects were notably good for Hebei and Shanxi provinces, with the correlation coefficients for the interannual regional average wind speed time series being 0.65 and 0.64, respectively. The reconstruction effects of surface wind speed differed across seasons, with spring and summer reconstructions showing the highest correlation with reanalysis data. The correlation coefficients for all seasons across most regions in China ranged between 0.4 and 0.8. Among the reconstructed seasonal wind speeds for the six provinces/autonomous regions, Shanxi Province in spring exhibited the highest correlation with the reanalysis, with a coefficient of 0.61. The large-scale climate variability indices showed good reconstruction effects on the annual mean wind speed in China, and could explain the interannual variability trends of surface wind speed in most regions of China, particularly in the main wind energy provinces/autonomous regions.

    Effects of Different Exogenous Substances on Seed Germination of Isatis indigotica Under Drought Stress and Chemical Composition of Isatis indigotica leaves

    Wang HaoQin JingjingShi ZheSong Jiayao...
    66-75页
    查看更多>>摘要:This study was to investigate the effects of three exogenous substances on chemical constituents of Isatis indigotica leaves and their efficacy in alleviating drought stress, and explore the methods of applying exogenous substances to efficient cultivation of Isatis indigotica. Polyethylene glycol (PEG) was used to simulate drought stress to deal with seeds of Isatis indigotica at the germination stage (concentration: 0, 10%, 15%, and 20%). Simultaneous operation of exogenous growth regulators [microbial inoculum (MI), γ-aminobutyric acid (GABA) and salicylic acid (SA)] and PEG were implemented in seeds of Isatis indigotica. The effects of drought stress and the mitigation of exogenous substances were observed by statistics of seed germination potential, germination rate, hypocotyl length, and radicle length of each treatment. The effects of exogenous substances on the content of alkaloids, crude protein and free amino acids in the leaves of Isatis indigotica grown in a greenhouse were determined after spraying exogenous substances on the plants. The differences of germination potential, germination rate, hypocotyl length, and radicle length among 15% PEG stress treatment, 10% PEG stress treatment and the control were significant (P<0.05). According to the predesigned germination standard, the seeds did not germinate under 20% PEG stress treatment. When the PEG concentration was 15%, the results of seed germination potential and germination rate after adding MI were significantly different from those under stress alone (P<0.05). When exposed to 10% PEG stress, the supplementation of GABA led to a notable increase in radicle length of Isatis indigotica seeds, showing significant differences compared to other three treatments. The application of MI and GABA under 15% PEG stress resulted in a significant increase in the radicle and hypocotyl length of Isatis indigotica seeds compared to other two treatments. The content of the total alkaloids in leaves of Isatis indigotica was significantly increased after spraying GABA. Meanwhile, the contents of crude protein and the total free amino acids were kept constant after spraying exogenous substances. Application of MI and GABA could alleviate drought stress of Isatis indigotica. The content of the total alkaloids in leaves of Isatis indigotica could significantly increase after spraying GABA.

    Preparation of Ephedra houttuynia Granule and Its Therapeutic Mechanism of Anti-Mycoplasma gallisepticum Infection Based on Network Pharmacology

    Zhang HuanqiHan MingdongMiao YusongXu Tianqiao...
    76-85页
    查看更多>>摘要:The use of Chinese herbal medicines can replace antibiotics that cause drug-resistance problems, which are currently necessary for disease control. In this paper, a traditional Chinese medicine compound named Ephedra houttuynia granule for the treatment of Mycoplasma galliscepticum (MG) infection was prepared. Furthermore, its action mechanism was explored through network pharmacology. The optimal extraction and granulation processes of the compound were determined by high performance liquid chromatography (HPLC) method and L9 orthogonal test, and in the treatment experiment, Ephedra houttuynia granule showed a significant therapeutic effect on MG infection. In the study of network pharmacology, the results showed that the core targets of Ephedra houttuynia granule against MG infection were vascular endothelial growth factor (VEGFA), fos proto-oncogene (FOS), prepro-coagulation factor Ⅱ(F2), etc., the gene ontology/kyoto encyclopedia of genes and genomes (GO/KEGG) analysis results indicated that the signaling pathways of neuroactive ligand receptor interaction, cAMP, IL-17, T cell receptor, and tumor necrosis factor (TNF) might involve in anti-MG infection. In conclusion, this study would provide a new idea for elucidating the action mechanism of other diseases in veterinary clinic, which had a certain guiding significance.

    Pharmacokinetic Characterization of Xylazine in Goats with Simultaneous Anesthesia Studies

    Gao TiantianLiu YongtiZhou LuluLi Yanan...
    86-96页
    查看更多>>摘要:The aim was to study the pharmacokinetics of xylazine as a stable anesthetic in goats. In this study, goats were injected with xylazine at the rate of 0.3 mL·kg-1 intramusculally, and blood samples were collected at 1, 3, 5, 10, 20, 30, 45, 60, 90, 120, 180, and 240 min after administration, respectively. Xylazine was extracted by liquid-liquid extraction and separation method, and blood concentration was determined by high performance liquid chromatography (HPLC). The pharmacokinetic characteristics of xylazine in healthy goats were analyzed by pharmacokinetic software. The results showed that the chromatographic peak time of xylazine chromatography was 9-11 min. The specificity of the method was good. The linear correlation coefficient R² of the standard curve was 0.9982 when the concentration of xylazine was in the range of 10-1×1000 ng. The pharmacokinetic model of xylazine in goats was a one-chamber model with first-order rate absorption, distribution half-life t1/2Ka was (0.49±0.041) min, elimination half-life t1/2Ke was (23.3±2.5) min, and the peak time (Tp) of the highest concentration was (2.8±0.2) min. The total drug clearance CL/F was (0.00016±0.000016) mg·kg-1·min-1 (ng·mL-1), and the minimum effective blood concentration was 56.6 ng·mL-1, which was consistent with the clinical anesthetic effect. The results showed that xylazine had the characteristics of rapid absorption, wide distribution, short peak time, slow clearance rate, and long anesthetic time in goats, which could be used as the basic drug for the development of goat complex anesthetic preparation.