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国际中医中药杂志
中国中医研究院中医药信息研究所
国际中医中药杂志

中国中医研究院中医药信息研究所

曹洪欣

月刊

1673-4246

guowaiyixue@yahoo.com.cn

010-64014411-3225

100700

北京市东直门内南小街16号中国中医科学院中医药信息研究所

国际中医中药杂志/Journal International Journal of Traditional Chinese Medicine北大核心CSTPCD
查看更多>>中华人民共和国卫生部主管，中华医学会、中国中医科学院中医药信息研究所主办。本刊以反映国内外中医药学研究的最新进展，促进国内外中医药学的学术交流为己任，以中医药科研、临床、生产、管理工作者为主要对象，介绍中医药学领域有关的新进展、新技术和新方法。开设了专题论坛、特别报道、临床研究、实验研究、临床调研、思路与方法、研究动态、信息研究、临床报道、临证心得、综述等栏目。为中国科技论文统计源期刊（中国科技核心期刊）。
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    《太平圣恵方》小儿病方剂剂型与服用特色探析

    赵雪淳张岚谢荣鑫郁晓兰...
    273-277页
    查看更多>>摘要：小儿生理、病理的特殊性决定了小儿病治疗过程中需格外重视调护。本文从方剂剂型及数量、送服汤液、服用药量、服药温度、服药时间、服药频率及程度等方面探讨《太平圣恵方》小儿病方剂剂型及服用特色。《太平圣恵方》治疗小儿病具有剂型丰富，内外治并重；重视脾胃的顾护，服药量轻而力专，强调中病即止；服药方式顺应小儿生理和病理特性等特点。 The particularity of children's physiology and pathology determines that doctors should pay special attention to nursing in the process of treating pediatric diseases. This article discussed the dosage form and taking characteristics of pediatric prescriptions in Tai Ping Sheng Hui Fang from the aspects of dosage form and quantity, decoction, dosage, temperature, time, frequency and degree. It has been concluded that Tai Ping Sheng Hui Fang is rich in dosage forms, both internal and external treatment paying attention to the care of the spleen and stomach, taking medicine in a light and specialized manner, and emphasizing the end of the disease the way of taking medicine conforms to the physiological and pathological characteristics of children.

    《太平圣恵方》儿科方剂剂型服药方法

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    基于“五气之溢”理论探析代谢性疾病前状态

    贺晴李梓荣杨俏丽林璟...
    278-282页
    查看更多>>摘要：代谢性疾病前状态是物质代谢紊乱且尚未达到疾病理化指标的机体状态，而糖代谢异常往往是代谢紊乱的关键环节。中医学中，脾的健运是精微物质生成与输布的基石，本文基于《内经》“五气之溢”理论，以脾备化失度为起点，对代谢性疾病前状态加以探讨，以期为临床代谢性疾病防治提供新的思路与方向。 The pre-metabolic disease state is the body state of substance metabolism disorder that has not yet reached the physical and chemical indicators of the disease, and abnormal glucose metabolism is often the key link of metabolic disorder. In TCM, the healthy function of the spleen is the cornerstone of the production and distribution of fine substances. This article discussed the pre-metabolic disease state based on the theory of "overflow of Five Qi" in the Nei Jing, taking the loss of spleen preparedness as the starting point, in order to provide new ideas and directions for the prevention and treatment of clinical metabolic diseases.

    脾（中医）五气之溢代谢性疾病病前状态五脏

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    基于通经柔筋法探讨火针治疗中风软瘫的理论思考

    沈琳博谢媛付渊博温雅丽...
    283-287页
    查看更多>>摘要：中风软瘫以中风后患者身体运动功能异常，肌肉张力下降为主要表现，是中风后较为常见的病理状态，软瘫时间越长预后越差。中医理论认为，中风主因“虚、风、火、痰、瘀、气”而发病，当邪气搏结、阻于经络则使气血凝滞、经筋损伤、肌肉痿废，导致中风软瘫，因此需立以通经柔筋为其主要治法。火针治疗中风软瘫具有通经温阳、柔筋止痛的作用，可温阳散寒、充养经脉经气、松解筋脉结节、推动气血运行、濡养四肢百骸。火针疗法可促进神经通路的恢复，加强局部新陈代谢，改善局部肌张力，从而恢复肢体功能。火针治疗中风后软瘫的高质量临床研究、选穴规律、规范化操作手法等有待进一步深化。 Stroke flaccid paralysis is stroke patients with abnormal physical movement function and muscle tone decline as the main performance and is a kind of common pathological state after apoplectic stroke. The longer the flaccid paralysis is, the worse the prognosis. The theory of TCM holds that stroke is mainly due to "deficiency, wind, fire, phlegm, stasis, qi", and when the pathogenic factor accumulate and block the meridians, which would cause blood stagnation, muscle and tendon damage and flaccidity, resulting in stroke paralysis. Therefore, it is necessary to set up the "Tongjing Roujin" (stimulating the muscle and nourishing the tendon) as its main treatment. Fire-needling has the effect of stimulating muscle, warming yang, nourishing tendon, and relieving pain in the treatment of stroke flaccid paralysis. It can warm yang and dissipate cold, replenish and nourish meridian qi, release muscle nodules, promote the circulation of qi and blood, and nourish all limbs and bones. Fire-needling therapy can promote the recovery of neural pathway, strengthen local metabolism, improve local muscle tension, and thus restore limb function. The high-quality clinical research, acupoint selection rules, and standardized operating techniques of fire-needling treatment for stroke flaccid paralysis need to be further deepened.

    中风（中医）软瘫期火针经筋通经柔筋

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    从“湿邪致病”探讨大肠息肉的发病

    孙梓宽苏晓兰班彦然龚卓之...
    288-292页
    查看更多>>摘要：大肠息肉的临床表现与湿邪黏滞、重着等特性相符，其好发人群的中医体质多与湿相关。易引发大肠息肉的肠道菌群失调、肠道微炎症状态、神经-内分泌-免疫网络和水通道蛋白异常等病理变化与湿邪现代机制研究结果相符。认为饮食不节、情志不畅等因素导致脾虚湿蕴，各种病邪交织形成有形异物是湿邪引发大肠息肉的中医病机。根据湿邪的致病特征，结合大肠息肉的发病因素，探讨湿邪在大肠息肉发病中的影响，以期为临床中大肠息肉的中西医结合诊疗提供有效的中医理论依据。 The clinical manifestations of colorectal polyps are consistent with the characteristics of dampness, stickiness and heaviness. The TCM constitutions in the prone population are mostly related to dampness. The pathological changes of intestinal flora imbalance, intestinal micro inflammation, neuroendocrine immune network and abnormal aquaporin in colorectal polyps are consistent with the research results of modern mechanism of dampness pathogen. This article believed that the TCM pathogenesis of colorectal polyps caused by damp pathogen is the accumulation of spleen deficiency and dampness caused by improper diet, poor emotion and other factors, and the interweaving of various diseases and pathogens to form tangible foreign bodies. According to the pathogenic characteristics of damp pathogen and the pathogenic factors of colorectal polyps, the influence of damp pathogen on the pathogenesis of colorectal polyps was discussed, in order to provide an effective TCM theoretical basis for the diagnosis and treatment of colorectal polyps in clinic.

    肠息肉湿邪中医体质学说发病特点

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    韩医药国际化发展现状分析及启示

    杨杨宋欣阳常久
    293-297页
    查看更多>>摘要：2003年，韩国政府提出韩医药国际化发展战略。韩国保健福祉部自此先后颁布了一系列相关支持政策，在推动韩医药吸引外国患者、开展国际教育，以及国际科研合作等方面取得丰硕成果。同时，韩药材、韩方健康功能食品、韩方化妆品也相继进军海外市场，获得了巨大的经济效益。中韩传统医学同源异流，韩医药国际化发展经验在一定程度上可为中医药国际化事业发展提供参考。 Korean government first proposed the internationalization of the Korean medicine strategy in 2003. During this time, the Ministry of Health and Welfare enacted a series of policies to support the internationalization of Korean medicine, which has led to fruitful results in attracting foreign patients, international education, and international research cooperation. At the same time, Korean medicinal materials, functional foods, and cosmetics have also entered overseas markets, reaping substantial economic benefits. As Chinese and Korean traditional medicines have the same origin and different flows, the experience of the international development of Korean medicine can provide a reference for the development of the internationalization of TCM to a certain extent.

    中药发展研究韩医药韩方产业国际化发展

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    232例维持性血液透析患者中医体质分布特点研究

    赵良斌吴玲杨菊孙进波...
    298-303页
    查看更多>>摘要：目的 分析探讨维持性血液透析（MHD）患者的中医体质分布情况。 方法 多中心横断面研究。收集四川省6家透析中心2021年10－12月MHD患者的临床资料、透析相关参数、理化检查数据，同时采用DS01-A舌面脉信息采集体质辨识体系进行中医体质辨识。 结果 纳入的232例MHD患者中，共分析出417种中医体质，其中平和质59例（25.43%）、偏颇质173例（74.57%）；实性体质中痰湿质［47例（20.26%）］，虚性体质中气虚质［86例（37.07%）］最为常见。不同性别、年龄、透析龄、是否合并糖尿病、高血压病或贫血的MHD患者，在体质分布上存在一定差异。 结论 MHD患者以偏颇质为主，性别、年龄、BMI、是否合并糖尿病或高血压病对中医体质分布有一定影响，不同中医体质可能对MHD患者的贫血产生影响。从中医体质角度对MHD患者进行干预，可能对患者预后有所裨益。 Objective To study the distribution of TCM constitutions in maintenance hemodialysis (MHD) patients. Methods This is a multicenter cross-sectional study. The general clinical data, dialysis-related parameters and physical and chemical examination data of MHD patients from 6 dialysis centers in Sichuan were collected. At the same time, DS01-A tongue and facial pulse information collection system was used for TCM constitution discrimination. Results A total of 232 MHD patients were enrolled , and 417 kinds of TCM constitutions were detected, including 59 patients (25.43%) with moderate constitution and 173 patients (74.57%) with biased constitution. Phlegm-dampness was the most common type of solid constitution 47 patients (20.26%). The most common deficiency constitution was qi deficiency 86 patients (37.07%). There were certain differences in the physical distribution of patients with different gender, age, dialysis age, BMI, and whether they had diabetes, hypertension or anemia. Conclusions The TCM constitutions of MHD patients are mainly biased constitution. Gender, age, BMI, diabetes or hypertension have a certain impact on the distribution of TCM constitutions. At the same time, different constitutions may have an impact on the anemia of MHD patients. The intervention of TCM constitutions on MHD patients may be beneficial to the prognosis of MHD patients.

    肾透析中医体质学说分布特点

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    穴位埋线联合血脂康胶囊对他汀不耐受高脂血症脾肾阳虚证患者脂代谢及抗氧化能力的影响

    卓蕴慧吕天娇季盛樊红雨...
    304-309页
    查看更多>>摘要：目的 探讨穴位埋线联合血脂康胶囊对他汀不耐受高脂血症脾肾阳虚证患者脂代谢及抗氧化能力的影响。 方法 随机对照试验研究。选取2020年7月－2022年6月上海中医药大学附属曙光医院、上海市普陀区中心医院及上海市徐汇区凌云社区卫生服务中心82例他汀不耐受高脂血症患者作为观察对象，采用单双球法随机分为2组，每组41例。2组均进行饮食、运动指导，对照组在此基础上口服血脂康胶囊，观察组在对照组基础上予以穴位埋线。2组均连续治疗3个月。分别于治疗前后进行中医证候评分，记录患者体重、腰围、BMI；采用全自动生化分析仪检测TC、TG、HDL-C、LDL-C水平，ELISA法检测血清SOD、GSH-Px、总抗氧化力（TAOP）水平，放射免疫法检测血清内皮素-1（ET-1）水平；彩色多普勒超声诊断仪检测肱动脉血流介导的内皮依赖性血管舒张功能（FMD）、硝酸甘油介导的非内皮依赖性血管舒张功能（NMD）。 结果 观察组治疗后畏寒肢冷［（1.27±0.12）分比（1.46±0.16）分，t=6.08］、便溏［（1.41±0.16）分比（1.63±0.18）分，t=5.85］、倦怠乏力［（1.45±0.15）分比（1.57±0.17）分，t=3.39］、眩晕［（1.15±0.11）分比（1.26±0.13）分，t=4.14］、食欲不振［（1.21±0.13）分比（1.39±0.15）分，t=5.81］积分低于对照组（P<0.01）；体重［（68.03±6.57）kg比（71.55±6.76）kg，t=2.39］、腰围［（85.13±4.63）cm比（87.35±4.85）cm，t=2.12］、BMI［（27.35±2.84）kg/m2比（29.18±3.05）kg/m2，t=2.81］水平低于对照组（P<0.05）；血清TC［（3.15±0.13）mmol/L比（3.38±0.17）mmol/L，t=6.88］、TG［（1.98±0.11）mmol/L比（2.21±0.15）mmol/L，t=7.92］、LDL-C［（2.46±0.26）mmol/L比（3.04±0.33）mmol/L，t=8.84］水平低于对照组（P<0.01），HDL-C［（1.88±0.24）mmol/L比（1.74±0.21）mmol/L，t=2.81］水平高于对照组（P<0.01）；血清SOD［（57.82±5.84）μg/L比（55.06±5.61）μg/L，t=2.18］、GSH-Px［（96.51±9.52）U/L比（92.26±9.25）U/L，t=2.30］、TAOP［（6.21±0.57）U/L比（5.94±0.54）U/L，t=2.20］水平高于对照组（P<0.05）；血清ET-1［（60.43±4.36）pg/L比（63.71±4.68）pg/L，t=3.28］水平低于对照组（P<0.01），FMD［（12.48±1.02）%比（11.34±0.95）%，t=5.24］、NMD［（15.12±1.24）%比（14.44±1.18）%，t=2.54］水平高于对照组（P<0.01）。 结论 穴位埋线联合血脂康胶囊可降低他汀不耐受高脂血症患者的中医证候积分，降低肥胖及血脂水平，增强抗氧化能力，改善血管内皮功能。 Objective To explore the influence of acupoint catgut embedding combined with Xuezhikang capsule on lipid metabolism and antioxidation effect in patients with statin intolerance and hyperlipidemia of spleen-kidney yang deficiency syndrome. Methods Randomized controlled trial. A total of 82 patients with statin intolerance and hyperlipidemia who were treated in Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine , Shanghai Puto Area Central Hospital, and Lingyun community health Service center, Xuhui District from July 2020 to June 2022 were selected and randomly divided into two groups with 41 patients in each group by odd-even ball method. The two groups were given diet and exercise guidance, and the control group was given Xuezhikang capsule orally on this basis, and the observation group was given poly(l-lactide-co-glycolide) (PLGA) thread acupoint catgut embedding on the basis of the control group. Both groups were treated continuously for 3 months. The TCM syndromes were scored before and after treatment, and body weight (BW), waist circumference (WC) and body mass index (BMI) were recorded. The levels of TC, TG, HDL-C and LDL-C were detected by fully automatic biochemical analyzer. The levels of serum SOD, GSH-Px and total antioxidant capacity (TAOP) were measured by ELISA. The level of serum endothelin-1 (ET-1) was assessed by radioimmunoassay. Color Doppler ultrasound diagnostic instrument was used to detect the brachial artery flow-mediated dilation (FMD) and nitroglycerin-mediated dilation (NMD). Results The scores of chilly sensation and cold limbs [(1.27±0.12) vs. (1.46±0.16), t=6.08], loose stool [(1.41±0.16)vs. (1.63±0.18), t=5.85], fatigue [(1.45±0.15) vs. (1.57±0.17), t=3.39], dizziness [(1.15±0.11) vs. (1.26±0.13),t=4.14] and loss of appetite [(1.21±0.13) vs. (1.39±0.15), t=5.81] in observation group after treatment were significantly lower than those in the control group (P<0.01). BW [(68.03±6.57)kgvs. (71.55±6.76)kg, t=2.39], WC [(85.13±4.63)cm vs. (87.35±4.85)cm, t=2.12] and BMI [(27.35±2.84)kg/m2 vs. (29.18±3.05)kg/m2, t=2.81]were lower than those in the control group (P<0.05). The levels of serum TC [(3.15±0.13)mmol/Lvs. (3.38±0.17)mmol/L, t=6.88], TG [(1.98±0.11)mmol/L vs. (2.21±0.15)mmol/L,t=7.92]and LDL-C [(2.46±0.26)mmol/L vs. (3.04±0.33)mmol/L,t=8.84]were significantly lower compared with those in control group (P<0.01) while the level of HDL-C [(1.88±0.24)mmol/Lvs. (1.74±0.21)mmol/L, t=2.81]was higher than that of the control group (P<0.01). The levels of serum SOD [(57.82±5.84)μg/Lvs. (55.06±5.61)μg/L, t=2.18], GSH-Px [(96.51±9.52)U/L vs. (92.26±9.25)U/L, t=2.30]and TAOP [(6.21±0.57)U/L vs. (5.94±0.54)U/L,t=2.20]were significantly higher compared to control group (P<0.05). Serum ET-1 [(60.43±4.36) pg/Lvs. (63.71±4.68) pg/L, t=3.28] level was significantly lower than that in control group (P<0.01), while FMD [(12.48±1.02)%vs. (11.34±0.95)%, t=5.24] and NMD [(15.12±1.24)% vs. (14.44±1.18)%, t=2.54] were significantly higher than those in control group (P<0.01). Conclusion The combination of acupoint catgut embedding and Xuezhikang capsule can reduce the scores of TCM syndromes, lower the obesity and blood lipids, enhance the antioxidant function and improve the vascular endothelial function in patients with statin intolerance and hyperlipidemia.

    高脂血症他汀不耐受穴位埋线疗法血脂康胶囊脂代谢

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    热敏灸配合痛点揉拨推拿手法治疗腰椎间盘突出症伴慢性下腰痛的临床研究

    李彭陈家平刘华许明高...
    310-314页
    查看更多>>摘要：目的 评价热敏灸配合痛点揉拨推拿手法治疗腰椎间盘突出症（LDH）伴慢性下腰痛（LBP）的临床疗效。 方法 随机对照试验研究。选择2020年1月－2022年12月六安市中医院91例患者作为观察对象，采用随机数字表法分为试验组46例和对照组45例。对照组予以常规推拿手法治疗，试验组予以热敏灸配合痛点揉拨推拿手法治疗。2组均连续治疗4周。采用VAS量表评估腰部疼痛程度，改良版Oswestry功能障碍指数（ODI）评估腰部功能障碍程度；采用血液流变仪检测血浆黏度、红细胞压积、全血低切黏度和全血高切黏度，评价临床疗效。 结果 试验组临床总有效率为93.48%（43/46）、对照组为77.78%（35/45），2组比较差异有统计学意义（χ 2=4.58，P=0.032）。治疗后，试验组VAS评分［（3.81±0.74）分比（4.29±0.85）分，t=2.88］、ODI评分［（8.79±2.65）分比（11.25±3.74）分，t=3.63］低于对照组（P<0.01）；血浆黏度［（1.35±0.06）mPa•s比（1.41±0.08）mPa•s，t=4.05］、红细胞压积［（37.46±2.38）%比（40.15±2.94）%，t=4.80］、全血低切黏度［（7.41±1.53）mPa•s比（8.64±1.72）mPa•s，t=3.61］和全血高切黏度［（3.81±0.29）mPa•s比（4.07±0.31）mPa•s，t=4.13］低于对照组（P<0.01）。 结论 热敏灸配合痛点揉拨推拿手法可有效缓解LDH伴慢性LBP患者腰部疼痛症状，改善腰部功能障碍及血流动力学状态，提高临床疗效。 Objective To evaluate the clinical efficacy of heat-sensitive moxibustion combined with kneading and massage manipulation at pain points in treating patients with lumbar disc herniation (LDH) and chronic low back pain (LBP). Methods Randomized controlled trial. A total of 91 patients with LDH and LBP who were admitted to Lu'an Hospital of Traditional Chinese Medicine from January 2020 to December 2022 were selected as the observation subjects. They were divided into the experimental group (46 cases) and the control group (45 cases) by random number table method. The control group was treated with conventional massage manipulation, while the experimental group was treated with heat-sensitive moxibustion combined with kneading and massage manipulation at pain points. Both groups received 4 weeks of continuous treatment. The degree of lumbar pain was evaluated with the Visual Analogue Scale (VAS), and the degree of lumbar dysfunction was evaluated with modified Oswestry Disability Index (ODI). Plasma viscosity, hematocrit, whole blood low shear viscosity and whole blood high shear viscosity were measured using a blood rheometer. Clinical efficacy was evaluated. Results The total clinical effective rates in the experimental group and the control group were 93.48% (43/46) and 77.78% (35/45), with a statistical significance between groups (χ2=4.58, P=0.032). After treatment, the VAS score [(3.81±0.74) vs. (4.29±0.85), t=2.88] and ODI score [(8.79±2.65) vs. (11.25±3.74), t=3.63] of the experimental group were lower than those of the control group (P<0.01). Plasma viscosity [(1.35±0.06) mPa•svs. (1.41±0.08) mPa•s, t=4.05], hematocrit [(37.46±2.38)% vs. (40.15±2.94)%,t=4.80], whole blood low shear viscosity [(7.41±1.53) mPa•s vs. (8.64±1.72) mPa•s,t=3.61] and whole blood high shear viscosity [(3.81±0.29) mPa•s vs. (4.07±0.31) mPa•s, t=4.13] were lower than those in the control group (P<0.01). Conclusion Heat-sensitive moxibustion combined with kneading and massage manipulation at pain points can effectively relieve LBP in patients with LDH and chronic LBP, improve lumbar dysfunction and hemodynamic status, and enhance clinical efficacy.

    椎间盘移位下腰痛热敏灸疗法痛点揉拨推拿手法

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    制何首乌不同提取物抗斑马鱼骨质疏松作用研究

    高媛媛阎芸芸陈卓然李霄...
    315-322页
    查看更多>>摘要：目的 研究制何首乌不同提取物抗斑马鱼骨质疏松作用。 方法 制备制何首乌、蒽醌和二苯乙烯苷部位和除蒽醌和二苯乙烯苷部位3种样品，应用泼尼松龙构建斑马鱼幼鱼骨质疏松症模型，设置正常对照组、模型组、依替膦酸二钠组和制何首乌不同提取物低、中、高剂量组，采用茜素红染色法考察各组斑马幼鱼头骨矿化面积和骨密度；采用碱性磷酸酶（AKP）和抗酒石酸酸性磷酸酶（TRACP）试剂盒检测斑马幼鱼中成骨细胞和破骨细胞酶活力；采用qRT-PCR法检测各组斑马鱼骨质疏松相关基因Runx2b、col1a2、sparc、vdrb mRNA表达情况。 结果 与模型组比较，制何首乌不同提取物剂量组头骨矿化面积和骨密度升高（P<0.01）；制何首乌、蒽醌和二苯乙烯苷部位和除蒽醌和二苯乙烯苷部位（中、高剂量）组斑马鱼AKP活力升高（P<0.01），TRACP活力降低（P<0.01）；制何首乌不同提取物组斑马鱼幼鱼 Runx2b、col1a2、sparc、vdrb mRNA水平升高（P<0.01）。 结论 制何首乌、蒽醌和二苯乙烯苷部位和除蒽醌和二苯乙烯苷部位均具有较好的抗骨质疏松作用，提示何首乌中除蒽醌和二苯乙烯苷类成分是抗骨质疏松主要药效成分外，还有其他化学成分具有抗骨质疏松的效果。 Objective To study on anti-osteoporosis effect of different extracts of Polygoni Multiflori Radix Praeparata on zebrafish. Methods Three kinds extracts of Polygoni Multiflori Radix Praeparata, anthraquinone and stilbene glycosides and the removal of anthraquinone and stilbene glycosides were prepared. Prednisolone was used to construct the osteoporosis model of young zebrafish. Normal control group, model group, disodium etidronate group and low-, medium- and high-dosage groups of different extracts of Polygoni Multiflori Radix Praeparata were set up. Alizarin red staining was used to investigate the mineralized skull area and bone density of juvenile zebrafish in each group. Alkaline phosphatase (AKP) and tartrate-resistant acid phosphatase (TRACP) kits were used to detect the activity of osteoblast and osteoclast enzymes in zebra larvae. The qRT PCR method was used to detect the mRNA expressions of osteoporosis related genes Runx2b, col1a2, sparc, and vdrb in each group of zebrafish. Results Compared with model group, the skull mineralized area and bone mineral density in different extracts of Polygoni Multiflori Radix Praeparata significantly increased (P<0.01). Polygoni Multiflori Radix Praeparata, anthraquinone and stilbene glycosides and the removal of anthraquinone and stilbene glycosides (medium- and high-dosage) could significantly increase the AKP activity of zebrafish (P<0.01), and lower the TRAP activity of zebrafish (P<0.01) the mRNA expression levels of Runx2b, col1a2, sparc and vdrb in juvenile zebrafish osteoporosis model were significantly up-regulated by different extracts of Polygoni Multiflori Radix Praeparata. (P<0.01). Conclusion Polygoni Multiflori Radix Praeparata, Anthraquinone and stilbene glycosides and the removal of anthraquinone and stilbene glycosides show better anti-osteoporosis effects. The comparison of the efficacy of three extracts from Polygonum multiflorum shows that in addition to anthraquinone and stilbene glycosides, other chemical components of Polygonum multiflorum have anti-osteoporosis effects.

    制何首乌斑马鱼骨质疏松泼尼松龙

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    加味通脉化癥汤干预Wnt/β-catenin通路促进子宫腺肌病模型小鼠病灶细胞凋亡的机制研究

    刘洋张科科王润涵师伟...
    323-329页
    查看更多>>摘要：目的 研究加味通脉化癥汤干预Wnt/β-连环蛋白（β-catenin）通路对子宫腺肌病（AM）小鼠病灶细胞凋亡的影响，探讨其作用机制。 方法 采用他莫昔芬造模法建立AM小鼠模型。将造模成功的21只小鼠按随机数字表法分为模型组、加味通脉化癥汤组、孕三烯酮组各7只，另设7只雌性小鼠为空白组。加味通脉化癥汤组灌胃加味通脉化癥汤36.51 g/kg，1次/d；孕三烯酮组灌胃孕三烯酮0.32 mg/kg，2次/周；空白组和模型组灌胃等体积的生理盐水，1次/d。干预2个月后，采用HE染色观察子宫组织形态，采用ELISA法检测血清糖类蛋白125（CA125）、泌乳素（PRL）水平，采用PCR检测子宫组织Wnt3a、β-catenin mRNA水平，采用Western blot法检测子宫组织Wnt3a、β-catenin、Bax、Bcl-2蛋白表达。 结果 与模型组比较，加味通脉化癥汤组小鼠血清CA125、PRL水平降低（P<0.05），子宫组织Wnt3a、β-catenin mRNA水平降低（P<0.05），Wnt3a、β-catenin、Bcl-2蛋白表达降低（P<0.05），Bax蛋白表达升高（P<0.05）。 结论 加味通脉化癥汤通过降低AM模型小鼠中血清CA125和PRL水平，缓解病灶进展，并可下调Bcl-2表达和上调Bax表达，促进小鼠异位病灶细胞凋亡，其作用机制可能与抑制Wnt/β-catenin通路相关蛋白表达有关。 Objective To study the effects of intervention of Jiawei Tongmai Huazhi Decoction in the Wnt/β-catenin pathway on apoptosis of lesion cells in mice with adenomyosis (AM) To discuss its mechanism of action. Methods The AM mouse model was established using tamoxifen. The mice were divided into model group, Jiawei Tongmai Huazhi Decoction group, and progesterone group according to random number table method, with 7 mice in each group. Additionally, a blank group of 7 female mice was set up. Jiawei Tongmai Huazhi Decoction group received oral administration of Jiawei Tongmai Huazhi Decoction at a dosage of 36.51 g/kg/day, once daily. The progesterone group received oral administration of progesterone at a dose of 0.32 mg/kg twice a week. The blank group and model group received oral administration of the same volume of physiological saline once daily. After 2 months of intervention, the morphology of uterine tissues was observed by HE staining. The levels of carbohydrate antigen 125 (CA125) and prolactin (PRL) in the serum were measured by ELISA. The mRNA levels of Wnt3a and β-catenin in uterine tissues were determined by PCR. The protein expressions of Wnt3a, β-catenin, Bax, and Bcl-2 in uterine tissues were detected by Western blot. Results Compared with the model group, the levels of serum CA125 and PRL were reduced in the Jiawei Tongmai Huazhi Decoction group (P<0.05). The protein expressions of Wnt3a, β-catenin, and Bcl-2 were also reduced (P<0.05), the protein expressions of Wnt3a, β-catenin, and Bcl-2 decreased (P<0.05), while the protein expressions of Bax increased (P<0.05). Conclusion Jiawei Tongmai Huazhi Decoction alleviates the progression of lesions by reducing serum CA125 and PRL levels in AM model mice, and can down-regulate Bcl-2 expression and up-regulate Bax expression, promoting apoptosis of ectopic lesion cells in mice. Its mechanism of action may be related to the inhibition of Wnt/β-catenin pathway related expression proteins.

    子宫腺肌病加味通脉化癥汤Wnt信号通路细胞凋亡小鼠

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