查看更多>>摘要：Objective: To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract. Methods: Huh7.5.1 cells were used to establish the HCV2a virus infection model. Cell survival rate (％) and Renilla Luciferase Assay Kit (％) were calculated by Celltiter-GLO Assay for evaluating CC50, EC50 and SI values. To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity, western-blot was used to detect the protein expression levels of NS5A, NS3 and NS5B. Results: the CC50, EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml, 1.90g/ml and 67.90 respectively. The EC50 after 24h, 48h and 72h administration were 18g/ml, 5.8g/ml and 2.3g/ml respectively. Within the range of drug concentration, the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship, but had no obvious effect on the expression of NS3 protein. Conclusion: The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B, and the virus has no tolerance to the aqueous extract of Hehuan Yin.
查看更多>>摘要：Objective: To prepare cationic liposome nanoparticles loaded with survivin -siRNA and Cnidium monnieri based on the ability of liposomes to contain both water-soluble and lipid soluble components. Methods: The preparation technology of Osthol cationic liposomes was optimized by orthogonal test with membrane material ratio, drug lipid ratio, ultrasonic time and steaming temperature as factors. The volume ratio of HA-siRNA to protamine and the ratio of HA-siRNA protamine complex to liposome were investigated by control variable method with potential and particle size as indexes. The particle size and zeta potential were measured by potentiometric particle size analyzer, and the shape was observed by transmission electron microscope; The absorbance of different concentrations of FAM-Survivin-siRNA standard solution was measured by microplate analyzer, and the entrapment efficiency of cationic liposomes loaded with FAM-Survivin-siRNA and osthole was calculated. Results: The optimum preparation conditions of osthole loaded cationic liposomes were as follows: the ratio of membrane to material was 3:1, the ratio of drug to lipid was 1:5, the steaming temperature was 30℃, the ultrasonic time was 70 min, and the encapsulation efficiency was 78.34％. The optimum preparation conditions of osthole loaded cationic liposomes loaded FAM-Survivin-siRNA were as follows: the volume ratio of Survivin-siRNA to protamine was 1:1, Protamine complex 25µg. Add 50µL cationic liposomes. The particle size is 132.3±0.2nm, zeta potential is 43.15±0.05mv, and its shape is irregular round; According to the standard curve, the entrapment efficiency of cationic liposome nanoparticles co loaded with Survivin-siRNA and osthole was 81.34 ± 0.041％. Conclusion: The prepared cationic liposome nanoparticles loaded with Survivin-siRNA and osthole have good encapsulation efficiency, particle size and zeta potential.
查看更多>>摘要：Objective: To observe the effect of MJPC cataplasm on the content of epithelial-derived cytokines in lung, skin and intestine of asthmatic mice. Methods: C57/BL6 female mice were randomly divided into four groups: control group, asthma model group, dexamethasone group and MJPC cataplasm group. Ovalbumin sensitized and challenged asthmatic mouse models were established. The spleen index was calculated, and HE staining was used to observe the pathological change in lung tissues. The ova-specific IgE in the mouse serum and the content of TSLP in lung, skin and intestine were detected by enzyme-linked immunosorbent assay ( ELISA). The expressions of TSLP mRNA and IL-33 mRNA in skin and intestinal tissue were detected by qRT-PCR. Results: Compared with the control group, the spleen index of mice in asthma model group was increased. Vascular congestion and edema, inflammatory cell infiltration and bronchial wall thickening were observed. The expressions of IgE in the mouse serum were significantly increased, and the content of TSLP in lung and skin tissue increased, but that in intestine tissue did not change significantly. The expression of TSLP mRNA was up-regulated in skin and intestinal tissues. The expression of IL-33 mRNA was up-regulated in skin tissue, but not in intestine, and the differences were statistically significant(P<0.05);Compared with the model group,MJPC cataplasm could decrease the spleen index and the expression of IgE in the mouse serum, improve the pathological damage of lung tissue in asthmatic mice, reduce the content of TSLP in lung, skin and intestinal tissue, increased the expression of TSLP mRNA in skin tissue, and down-regulate the expression of Il-33 mRNA in skin tissue and the expression of TSLP mRNA and IL-33 mRNA expression in intestinal tissue(P<0.05). Pearson correlation coefficient (r) of the content of TSLP between lung and skin was 0.689, that between lung and intestinal was -0.163, and that between skin and intestinal was -0.163, and the differences were not statistically significant(P>0.05). Conclusion: MJPC cataplasm improve airway inflammation by inhibiting the content of epithelial-derived cytokines on the "lung-skin-intestine" axis of asthmatic mice, and achieve the effect of treating asthm.
查看更多>>摘要：Objective: Determine the urinary biomarkers and pathogenesis of pulmonary fibrosis rats, and elaborate the intervention mechanism of DanBei YiFei formula. Methods: Bleomycin was injected into the trachea to induce pulmonary fibrosis in rats after anesthesia, and the diagnostic indexes of clinical pulmonary fibrosis, including superoxide dismutase, glutathione and malondialdehyde, were measured. High-throughput metabolic data of rats with pulmonary fibrosis were obtained by the latest high-resolution liquid-mass spectrometry technology, the multidimensional data were processed by Chemometrics algorithm to screen biomarkers related to pulmonary fibrosis. While, metabolic function indexes of rats after administration was observed, and the effective mechanism of DanBei YiFei formula on pulmonary fibrosis was expounded. Results: The clinical biochemical indexes showed that there were significant differences in metabolism in the model group, which confirmed the success of the preparation of the model of pulmonary fibrosis. Metabolisms research showed that the metabolic contour of the rats with pulmonary fibrosis was found to be significantly deviated, and the metabolism in vivo was abnormal. After the DanBei YiFei formula was given, the overall metabolic contour of the rats showed a trend of back modulation, and developed in the direction of healthy rats. With database matching and data processing 12 biomarkers, including Fumaric acid, Arginine and Spermidine, were obtained which were radically different from those of healthy rats and pulmonary fibrosis rats. Conclusion: DanBei YiFei formula has definite therapeutic effect on pulmonary fibrosis rats. Regulation of Tricarboxylic acid cycle and Arginine metabolic pathway may be the mechanism of its treatment of pulmonary fibrosis.
查看更多>>摘要：Objective: To analyze the clinical characteristics of patients with type 2 diabetes mellitus (T2DM) with acute coronary syndrome (ACS), the global registry of acute coronary events (GRACE)score,the thrombolysis in myocardial infarction (TIMI) score and clinical prognosis. Method: The study was a retrospective one-center observational study, continuous inclusion of 600 ACS patients diagnosed by coronary angiography in our hospital from October 2018 to July 2019.Collect general clinical data, laboratory examination results, imaging data and interventional treatment data of all patients. Were divided into: T2DM with ACS group (group DA) and non-T2DM with ACS (group NDA) according to whether or not they were associated with T2DM. According to the GRACE、TIMI score, the two groups were divided into high risk group, middle risk group and low risk group.All patients underwent coronary angiography to calculate the number of vascular lesions and Gensini scores. Design questionnaire, after discharge to 2 groups of patients by telephone or outpatient follow-up average of 10 months, statistics of the occurrence of MACE events. Result: Among the 600 patients included in the study ,362 were male (60.3％) and 238 were female (39.7％) with mean age (64.7±10.3) years. The baseline data showed that the G、TG、UA、CR levels were higher in the DA group than in the NDA group; the proportion of men was lower than in the NDA group. The results of coronary angiography showed that the Gensini score of DA group was higher than that of NDA group, and the proportion of single lesion was lower than that of NDA group. The binary Logistic regression analysis suggested that age and CRP were independent risk factors for MACE events in patients with T2DM. GRACE risk stratification showed that the proportion of high risk group in DA group was significantly higher than that in NDA group, and there was no significant difference between low and middle risk group. TIMI risk stratification showed that the proportion of high risk group in DA group was significantly higher than that in NDA group, while the proportion of low and middle risk group was lower than that in NDA group. The ROC curve shows that the area (AUC) below the ROC curve that GRACE、TIMI score predicted the occurrence of MACE events in patients with T2DM and ACS was 0.707 and 0.586. Conclusion: Patients with T2DM and ACS had higher clinical risk stratification than without T2DM. GRACE score compared with the TIMI score had better predictive value for the occurrence of MACE events after discharge of T2DM with ACS patients.
查看更多>>摘要：Objective: To observe the effect of intradermal needle therapy on back-shu points of five zang organs in treating chronic fatigue syndrome (CFS) and provide evidence for the usage of unilateral back-shu point. Methods: Patients were randomly divided into bilateral back-shu point group, unilateral back-shu point group and non-acupoint group, remove shedding cases, each group remaining 30 patients. In bilateral back-shu point group, both sides of back-shu points of five zang organs were selected. In unilateral back-shu point group, only one side was selected, left or right alternately. The location where non-acupoint group inserted by intradermal needles were outward from the back-shu points, left or right alternately. Fatigue Scale-14 (FS-14) was used to evaluate the fatigue level and the scores' improvement of physical fatigue and mental fatigue revealed the therapeutic effect on body constituents and spirits of CFS patient. Results: After the 8-week intradermal needle therapy, the total effective rate was 83.33％ in the bilateral back-shu point group, which was 76.67％ in the unilateral back-shu point group and 50.00％ in the non-acupoint group. The curative effects of the two groups whose back-shu points of five zang organs were inserted by intradermal needles were better than the non-acupoint group(P<0.05). There was no significant difference in clinical efficacy between the bilateral back-shu point group and the unilateral back-shu point group (P>0.05). There were statistical differences in FS-14 questionnaires including total, physical and mental scores between before and after the treatment in both the two back-shu point groups(P<0.05). Only the scores of total and physical fatigue in decreased in non-acupoint group(P<0.05), which means it couldn't help to alleviate mental fatigue statistically(P>0.05). Conclusions: (1) The intradermal needle therapy on back-shu points of five zang organs for CFS has a satisfying clinical efficacy in curing tiredness. It can help to reduce both physical and mental fatigue for the patients. Hence, it can regulate both body constituents and spirits simultaneously. (2) Needling the unilateral acupoints can get similar clinical efficacy to the bilateral points. It reveals that we can cut cost by reducing the consumption of the amount of intradermal needles used on one side. (3) Both the groups of back-shu points get better clinical efficacy than the non-acupoint group dramatically. The reason of why the intradermal needle can treat CFS maybe that back-shu points of five zang organs can regulate the functions of five zang organs characteristically.
查看更多>>摘要：Objective: To explore the clinical effect of Chinese medicine aerosol fumigation on demodex infection related meibomian gland dysfunction. Methods: In this prospective study,60 patients(120 eyes) were selected and randomly divided into 2 groups: the treatment group(30 cases, 60 eyes) was treated with Chinese medicine aerosol fumigation and meibomian gland massage, the control group(30 cases, 60 eyes) was treated with meibomian gland massage. Observation of Demodex count, symptom scores ,break-up time (BUT), Schirmer I test (SIT), meibomian gland secretion function scores and eyelid fat traits scores were used to compare the difference between before and after treatment. Results: There was no significant difference in Demodex count, symptom scores ,BUT, SIT, meibomian gland secretion function scores and eyelid fat traits scores between the 2 groups before treatment(P>0.05).After treatment, symptom scores ,meibomian gland secretion function scores and eyelid fat traits and Demodex count of the treatment group were significantly reduced(P<0.01). Conclusion: Chinese medicine aerosol fumigation can relieve symptoms of of demodex infection related meibomian gland dysfunctionand reduce the number of ocular Demodex.
查看更多>>摘要：Objective: To evaluate the clinical efficacy and safety of Chinese patent medicine Bupi Yichang Pill in the treatment of ulcerative colitis. Method: Use a computer to retrieve English and Chinese database(PubMed, EMBASE, CNKI, VIP, and Wanfang) from the establishment of the database to May 2020. The literature of clinical randomized controlled trials of Chinese patent medicine Bupi Yichang Pill for the treatment of ulcerative colitis were collected. Two researchers independently extracted the data and evaluated the quality of the included studies and meta-analysis was conducted by Using Stata 16.0 software, and the results were graded and evaluated by GRADE. Result: Eight studies with 835 patients were included. The overall analysis showed that Bupi Yichang Pill was more effective than mesalazine sustained-release granules both in mucosal score under colonoscopy (SMD = -0.93, 95％ CI [-1.20, -0.67]) and total efficiency (RR= 1.26, 95％ CI [1.11, 1.43]). Compared with sulfasalazine, Bupi Yichang Pill produced a more significant effect in terms of total efficiency (RR = 1.13, 95％ CI [1.05, 1.21]). In the comparison of total symptom score, Bupi Yichang Pill group was lower than mesalazine sustained-release granules group(SMD = -0.94, 95％ CI [-1.16, -0.72]). The more adverse effect occurred in the mesalazine group when comparing with the Bupi Yichang Pill group (RR = 0.46, 95％ CI [0.16, 1.28]). Conclusion: Bupi Yichang Pill with great safety can effectively improve the clinical effectiveness of ulcerative colitis but the conclusions above are still needed a further study through the higher quality of evidence.
查看更多>>摘要：Objective: The mechanism of Yupingfeng Powder in the treatment of non-small cell lung cancer was analyzed by network pharmacology and molecular docking method. Methods: Search the related literature and TCMSP database, query the chemical composition and action target of Yupingfeng powder, query the disease database of Genecards, OMIM, DisGeNET, Drugbank, etc., use "non-small cell lung cancer" as the keyword to search the disease-related target, select the intersection target with Yupingfeng powder as the research target, and use Cytoscape 3.7.2 software to construct the active component-target network map. The protein-protein interaction network map was constructed by using STRING database, and the treatment core targets were further screened by topological parameters, and GO analysis and KEGG signal pathway enrichment analysis were carried out. Finally, molecular docking was verified. Results: 37 active components of Yupingfeng Powder were screened, including 146 common targets and 44 core targets. GO analysis and KEGG analysis show that the core target participates in many biological processes such as angiogenesis and cell proliferation, and acts on a variety of signal pathways such as AGE-RAGE, IL-17, TNF, MAPK and so on. Molecular docking shows that the core compound has good docking activity with the target. Conclusion: Network pharmacological analysis shows that Yupingfeng powder in the treatment of non-small cell lung cancer involves multi-target and multi-pathway mechanism, and the drug and target have good docking activity, which can provide theoretical basis for follow-up experimental research.
查看更多>>摘要：Objective: To explore the general differentiation and treatment of insomnia by Professor Gao Ying through drug clustering and group correspondence analysis, and provide reference for clinical diagnosis and treatment. Methods: Collect retrospective case data from outpatient system, use SPSS20.0 software to perform frequency and cluster analysis on high-frequency symptoms and drug data, and perform corresponding analysis on the clustered drug syndrome groups. Results: A total of 349 consultations in 204 patients were included. Cluster analysis of 35 symptoms and 40 flavors with a frequency of more than 10％ resulted in a corresponding relationship between 7 symptom groups, 6 drug groups and 5 drug syndrome groups. The medicine symptom group has a high degree of matching; the doctors distinguish and tre at insomnia with calming, clearing heat, nourishing yin, liver, spleen, qi and phlegm as the core treatment, with consistent decoction, two to pill, lily ground Huang Tang, Lily Zhimu Decoction, Wendan Decoction, Sini San, Xiao Chai Hu Tang, Xiaoyao San, etc. are commonly used prescriptions; the physician's experience is to add or subtract Danshen and Zao Ren drink, which has a wide range of applicability to various insomnia syndrome. Conclusion: Based on the cluster analysis of drug symptoms and group correspondence analysis, it can reveal the pathogenesis, treatment and class information hidden in the data of drug symptoms, which can reflect the general law of physicians' syndrome differentiation and treatment of insomnia. This method has a reference for the exploration of TCM clinical experience significance; The results of this study can provide feedback to guide the clinical diagnosis and treatment of insomnia.
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维普