首页|Study on the in vitro activity of Hehuan Yin aqueous extract against hepatitis C 2a virus
Study on the in vitro activity of Hehuan Yin aqueous extract against hepatitis C 2a virus
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Objective: To study the anti-HCV activity and mechanism of Hehuan Yin aqueous extract. Methods: Huh7.5.1 cells were used to establish the HCV2a virus infection model. Cell survival rate (％) and Renilla Luciferase Assay Kit (％) were calculated by Celltiter-GLO Assay for evaluating CC50, EC50 and SI values. To observe the drug resistance of the virus to different concentrations of Hehuan Yin within 72 hours by detecting luciferase activity, western-blot was used to detect the protein expression levels of NS5A, NS3 and NS5B. Results: the CC50, EC50 and SI of Hehuan Yin against HCV2a were 132.50g/ml, 1.90g/ml and 67.90 respectively. The EC50 after 24h, 48h and 72h administration were 18g/ml, 5.8g/ml and 2.3g/ml respectively. Within the range of drug concentration, the aqueous extract Hehuan Yin had inhibitory effect on the expression of NS5A and NS5B proteins in a dose-effect relationship, but had no obvious effect on the expression of NS3 protein. Conclusion: The aqueous extract of Hehuan Yin may inhibit the replication of HCV2a virus by changing the protein expression levels of NS5A and NS5B, and the virus has no tolerance to the aqueous extract of Hehuan Yin.
Hehuan Yin aqueous extractHepatitis C 2a virusIn vitro experimentsAntiviral activity
School of Traditional Chinese Medicine,Southern Medical University,Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics,Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology,Guangzhou 510515,China
NETL
NSTL
万方数据
维普
