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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    COVID-19 sequelae: Clinical features and research progress

    Na LiZhe WangYun-Li BaoHai-Ru Tang...
    1-8页
    查看更多>>摘要：With the increasing number of COVID-19 patients cured and discharged, the emergence of associated sequelae has become a new global health crisis following acute infection. The sequela of COVID-19 involves many systems such as respiration, circulation, nerve, digestion, psychological cognition, and so on, with varying duration and severity, seriously affecting people's physical and mental health, as well as the development of society. This paper summarizes the clinical characteristics and research progress of COVID-19 sequelae through extensive review of relevant domestic and foreign literature, in order to provide reference for the development of a comprehensive and systematic management system.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Effect of Simiao Yongan Decoction on atherosclerotic carotid plaque in ApoE-/- mice via antagonizing Ox-LDL lipid metabolism pathway

    Zhao GaoXin-Rui XuQiu-Shuo JinHao Sun...
    9-15页
    查看更多>>摘要：Objective: To explore the intervention effects and mechanism of Simiao Yongan (SMYA) Decoction on carotid atherosclerotic plaque in ApoE knockout mice by antagonizing lipid metabolism of oxidation low density lipoprotein (Ox-LDL). Methods: ApoE-/- mice were fed high-fat feeding for 2 weeks, combined with perivascular collar placement (PCCP) and high fat feeding for 8 weeks to establish carotid AS plaque model. All the mouses were divided into 5 groups, after successful AS model preparation. The sham operation as well as model group intragastrically administered with deionized water. The rest mouses were intragastrically administered with SMYA decoction or Pioglitazone, or treated with Atorvastatin for 8 weeks. Then adopting automatic biochemical analyzer to test total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) content. The content of ox-LDL in serum , which was tested by enzyme-linked immunosorbent assay (ELISA). The pathological changes of carotid artery were observed by hematoxylin-eosin (HE) staining and the vascular parameters were measured. Immunohistochemical method and Western blot assay was used to detect the manifestations of protein include fatty acid binding protein 4 (FABP4) and peroxisome proliferator-activated receptor γ (PPARγ) as well as matrix metalloproteinase 2 (MMP 2). Results: Compared with the sham operation group, the serum ox-LDL content of the model group was increased, and the intima thickness (IT), intima-media thickness (IT/MT), plaque area (PA) as well as lumen area ratio of plaque vessels (PA/LA) were enlarged (P < 0.05). Immunohistochemistry and Western blot results showed that the relative expression of FABP4 and MMP2 in the model group increased markedly, while the relative expression of PPARγsignificantly decreased. Compared SMYA decoction group and Atorvastatin group as well as Pioglitazone with model group, the serum ox-LDL content was decreased (P < 0.05). In the mean time, the IT, PA, IT/MT and PA/LA in carotid artery were reduced (P < 0.05). Immunohistochemistry and Western blot results showed that the expression of FABP4 and MMP2 was reduced, while PPARγ protein expression increased of mouses in each administration group. Conclusion: SMYA decoction is able to antagonize Ox-LDL, increase the expression of PPARγ, decrease the level of FABP4, reduce MMP2 in carotid arteries of ApoE-/- atherosclerotic mice, and increase the stability of plaque.
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    Effect of TSG on tau phosphorylation via GSK-3β pathway

    Wan-Ying MengChao-Yu LiuXiao-Yan XiaYan-Bing Li...
    16-23页
    查看更多>>摘要：Objective: To observe how TSG interferes with Tau phosphorylation in Aβ25-35 induced dementia model via GSK-3β pathway. Methods: Thirty-six male SD rats aged 24 months were randomly divided into control group, sham group, model group, low-dose, medium-dose and high-dose of TSG groups (TSG, 0.033, 0.1, 0.3g kg-1), with 6 rats in each group. Model group and TSG dose groups were injected Aβ25-35 into hippocampus by brain stereoscopic locator to induce dementia model (sham group was injected with equal volume of normal saline). Six SD rats of the same age were selected as normal group. 14d after modeling by Aβ25-35, the rats was given TSG by gavage. once A day in each group for 4 weeks. After 4 weeks of treatment with TSG, HE staining was used to observe the structural changes of nerve cells in brain tissue, IHC was used to observe the expression of PKC and PKA protein in brain tissue, real-time PCR was used to observe the mRNA expressions of GSK-3β, PKA, PI3K, PKB and PKC, and Western blot was used to observe the expression of GSK-3β, P-tau, PI3K and PKB protein in hippocampus. Results: Compared with normal group, the number of nerve cells in model group was less, and the arrangement was sparse and disordered. The expression of PI3K, PKB mRNA were significantly decrease(P<0.01), the expression of GSK-3β, PKA mRNA was go up(P<0.05), the exPression of PKC mRNA was decreased(P<0.05), the protein expression level of PKC(P<0.01), PI3K (P<0.05) and PKB(P<0.01)were decreased, the expression levels of PKC protein in hippocampus and cortex were significantly decreased(P<0.01), the expression levels of PKA protein in hippocampus and cortex were go up(P<0.05).Compared with model group, the number of nerve cells in each administration group increased to a certain extent, the expression levels of P-tau, GSK-3β and PKA protein and mRNA were significantly decrease(P<0.05, P<0.01), the expression levels of PI3K,PKB and PKC protein and mRNA were significantly up-regulated (P<0.05,P<0.01). Conclusion: TSG can regulate GSK-3β, PI3K, PKC, PKB and PKA in the hippocampus of Aβ25-35 induced dementia model. By regulating the expression of these factors, the hyperphosphorylation of Tau protein can be inhibited and the phenomenon of hyperphosphorylation of Tau protein can be improved.
      原文链接:
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    Berberine protects diabetic nephropathy rats by inhibiting endoplasmic reticulum stress

    Yan-Fei WangHui-Juan YangHang-Lin LiWen-Wen Zhao...
    24-29页
    查看更多>>摘要：Objective: The aim of this study was to investigate the effects of Berberine (BBR) on Endoplasmic Reticulum Stress (ERS) in renal tissue of Diabetic Nephropathy (DN). Methods: The DN RAT model was induced by high glucose and high fat diet combined with intraperitoneal injection of Streptozocin. The experiment was divided into 3 groups: normal group (NC group), model group (DN group) and berberine intervention group (DN + BBR group)(n = 6) . DN + BBR group was treated with 200 mg/kg/d on the basis of the model of DN. NC group and DN group were treated with the same dose of sodium carboxymethyl cellulose. After 6 weeks treatment measured various indicators (include body weight, renal index (KI = kidney weight/body weight), fasting blood glucose (FBG), blood creatinine (SCR), urea nitrogen (BUN) and 24h urine protein (24h Pro)). The pathological changes of kidney were observed by HE, PAS and Masson staining. The changes of glomerulus and renal interstitium were observed by transmission electron microscope. The expression of PERK, IRE1, ATF6, CHOP and Caspase3 were detected by immunohistochemical staining. Results: (1) SCR, Bun, FBG and 24hUpro in DN group were significantly higher than those in NC group and the renal function of DN group severely impaired. Compare with DN group the renal function of DN + BBR group was significant improved. There were significant differences in SCR, Bun, FBG and 24hUpro in each group (p < 0.01) . (2) The results of HE, PAS and Masson staining showed that the glomerulus in DN group was more irregular and larger than that in NC group; the lumen of glomerulus became narrowed, diffuse mesangial matrix was increased and renal tubule was edematous. The glycogen deposits and collagen fibers in the renal interstitium were increased and inflammatory cells were infiltrated. After the berberine rescued, the glomerular condition of DN + BBR group was obviously improved; the edema of renal tubules was alleviated; the deposition of glycogen was decreased and the collagenous fibers accumulation is relatively reduced. (3) The results of transmission electron microscope showed that the podocytes of DN Group were irregular and a large number of podocytes fused and broken. The basement membrane was inhomogeneous and thickened. Whereas, the morphological function of podocyte of DN + BBR Group was improved. And the basement membrane was slightly thickened. (4) Immunohistochemistry shows that the expression of Chop, PERK, IRE1, ATF6 and Caspase3 in DN group was significantly increased and the DN + BBR group was contrarily. The difference of protein expression was statistically significant (p < 0.05). Conclusion: These findings provide insights into the BBR can significantly improve the structure and function of kidney in DN rats. The hypothesis is that BBR can suppress endoplasmic reticulum stress and reduce the apoptosis of kidney cells, thus prevent the progress of DN and protect the kidney tissue.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Analysis of hyperuricemia in physical examination population of a hospital in Haikou

    Xiao-Bo TangYing-Zi LinLiu-Ting LiuChan-Juan Zhao...
    30-34页
    查看更多>>摘要：Objective: Explore the influencing factors of Hyperuricemia (HUA) in natural people aged 35-74 in Haikou. Methods: Between June 2017 and December 2020, 8754 different study subjects aged 35-74 years were enrolled for the baseline survey at the physical examination center of a tri partite hospital in Haikou. Results: Overall detection rate of HUA was 18.5％, including 25.6％ in male and 10.4％ in female. HUA detection rates varied between the 35–45 and 46–55 age groups (P<0.05).The binary logistic results showed that high blood pressure, triglyceride, low density lipoprotein and creatinine were the risk factors of HUA (P<0.05). Conclusions: The baseline survey results show that the detection rate of HUA in a hospital in Haikou is relatively high. Men and groups with high blood pressure, creatinine, triglyceride and low-density lipoprotein should be the key groups for the early prevention and treatment of hyperuricemia(HUA).
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    • NETL
    • NSTL
    • 万方数据
    • 维普

    Clinical efficacy of Chinese herbal compound in the treatment of infertility with kidney deficiency: A meta-analysis

    Lei ZhangZhi-Juan WuJian-Wei Zhang
    35-41页
    查看更多>>摘要：Objective: Based on the current evidence system to evaluate the effect of traditional Chinese medicine compound on the clinical efficacy of infertility patients with kidney deficiency. Methods: Search the China Knowledge Resources Database (CNKI), Chinese Science and Technology Journal Database (VIP), China Academic Journal Database (Wanfang), PubMed, EMBASE, Ovid. The included studies are randomized controlled trials. The experimental group is Chinese medicine compound or Chinese medicine compound combined with western medicine, and the control group is western medicine. Two reviewers independently extracted and evaluated the literature data according to the Cochrane manual standards, and used Rev Man5.3 software for data synthesis and Meta-analysis. Results: 32 studies were selected, a total of 2715 cases of infertility patients. The clinical pregnancy rate of the experimental group and the control group[n=29, OR(95％CI)=2.47(2.07, 2.96), P<0.00001], the clinical total effective rate [n=20, OR(95％CI)=3.77(2.83, 5.03), P<0.00001], TCM syndrome score [n=14, MD (95％CI)=4.34 (3.08,5.61), P<0.00001], ovulation rate[n=20, OR(95％CI)=2.21(1.85,2.63), P<0.00001], the occurrence rate of two-way BBT [n=11, OR (95％CI)=2.65 (2.02, 3.48), P<0.00001]. The GRADE scoring results show that the level of evidence is low and very low. Conclusion: Traditional Chinese medicine compound can increase the clinical pregnancy rate and clinical total effective rate of infertility with kidney deficiency syndrome, improve the scores of Chinese medical syndromes, increase its ovulation rate and the occurrence rate of BBT bidirectional type, and confirm the clinical efficacy of Chinese medicine compound from the perspective of evidence-based medicine.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Expression and clinical significance of PRKCD in liver hepatocellular carcinoma analyzed based on bioinformatics

    Lian-Tao HuWen-Jun DengLuo SunShi-Zhen Lu...
    42-45页
    查看更多>>摘要：Objective: To analyze the expression of protein kinase C delta (PKCδ, PRKCD) in Liver hepatocellular carcinoma and its clinical significance based on bioinformatics, in order to provide a new direction for the study of therapeutic targets for Liver hepatocellular carcinoma. Methods: The PRKCD gene expression data and patient clinical information data in Liver hepatocellular carcinoma tissues and adjacent tissues were downloaded from The TCGA (The Cancer Genome Atlas) and UCSC Xena databases. The mRNA expression of PRKCD in Liver hepatocellular carcinoma was analyzed, and the protein expression of PRKCD in Liver hepatocellular carcinoma was analyzed by Human Protein Atlas (HPA) databases. Furthermore, its relationship with clinicopathological features and prognosis in patients with Liver hepatocellular carcinoma was analyzed. GSEA enrichment analysis were carried out for PRKCD in Liver hepatocellular carcinoma. Finally, the causes of changes in PRKCD expression were analyzed from the perspective of genetic and epigenetics based on collated liver hepatocellular carcinoma data. Results: Both the mRNA and protein expression level of PRKCD gene in Liver hepatocellular carcinoma tissues was significantly higher than that in adjacent tissues. Its expression level was correlated with TNM stage, Histologic grade,Alpha fetoprotein (AFP) and Living status in clinicopathological features, and its expression level has certain clinical diagnostic accuracy. Kaplan-Meier analysis showed that the prognosis of patients with low PRKCD expression was significantly better than that with high PRKCD expression. Univariate and multivariate Cox regression analysis showed that PRKCD was an independent prognostic factor in patients with Liver hepatocellular carcinoma, and it was also found that the risk ratio of TNM stage Ⅲ and stage IV survival curve (HR) was significantly greater than that of stage I and stage Ⅱ. GSEA analysis showed that it was enriched in cell cycles and DNA replication, and was positively correlated with PRKCD. Enrichment analysis found that PRKCD is mainly involved in the chemokine signaling pathway, NOD-like receptor signaling pathway, PPAR signaling pathway, adipocytokine signaling pathway and T-cell receptor signaling pathway. Finally, through genetic and epigenetic analysis, it is found that the increase in the number of copies of genes will increase the expression level of PRKCD, and the methylation level is negatively correlated with the expression level of PRKCD. Conclusion: PRKCD gene is upregulated in Liver hepatocellular carcinoma tissues, and its overexpression level is closely related to patient poor prognosis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Discussion on the mechanism of Erxian Decoction in the treatment of premature ovarian failure based on network pharmacology and molecular docking

    Yu LiXiao-Bin LiXiao-Jing Cao
    46页
    查看更多>>摘要：Objective: To explore the mechanism of Erxian Decoction in the treatment of premature ovarian failure. Methods: Based on the method of network pharmacology and molecular docking, the active ingredients of each drug in Erxian Decoction were obtained by searching the TCMSP database; the premature ovarian failure disease targets were collected from the GeneCards, OMIM, PharmGkb and Drugbank databases, and the active ingredients and the disease gene targets were collected Click the intersection to get the predictive target of Erxian Decoction for the treatment of premature ovarian failure. Use Cytoscape 3.8.0 to construct a "drug-component-target-disease" network; construct a protein interaction network (PPI) and streamline the core network through STRING database and Cytoscape; use R Studio software to enrich the Erxiantang treatment POF with GO And KEGG pathway enrichment analysis. Use molecular docking technology to verify the results of the "drug-component-target-disease" network. Results: 68 main active ingredients were screened, involving 182 gene targets, among which the main active ingredients include Quercetin, Luteolin, Kaempferol, etc.; The core target genes include RB1,TP53,FOS,CDKN1A,ESR1,AKT1,MAPK1,TNF, etc.;GO enrichment items were obtained, including the 2545 Biological Process (BP) ,89 Cellular Component (CC), 212 Molecular Function (MF); KEGG pathways, including PI3K-Akt signaling pathway, MAPK signaling pathway, and AGE-RAGE signaling pathway in diabetic complications. The verification of the molecular docking results indicated that the main active ingredient has a good binding activity with the core target. Conclusion: This study preliminarily revealed that Erxian Decoction may play a role in the treatment of POF through multi-component, multi-target, and multi-channel synergy, which provides a reference for the next in-depth research.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Physiological and pharmacological functions of G protein coupled receptor 124: A review

    Yi-Qian XuHao-Lin WuXing-Yue FanHao-Fei Fan...
    47-52页
    查看更多>>摘要：G protein-coupled receptors(GPCRs) are the largest protein superfamily in the body, expressed in various tissues and organs, and are currently one of the most important clinical drug targets. Recently, a class of GPCRs without endogenous ligands(orphan GPCRs) have been discovered. They exhibit different physiological functions in the body and act extensively on the cardiovascular and cerebrovascular systems. Among them, G protein-coupled receptor 124(GPR124) is an orphaned member of the G protein coupled receptor adhesion family that has attracted much attention. It plays a key role in promoting cerebral angiogenesis and maintaining the stability of the blood-brain barrier. It also associated with cardiovascular and cerebrovascular diseases such as cerebral ischemia and atherosclerosis. However, the role of GPR124 in these diseases, the associated signaling pathways, and possible drug intervention targets are still unclear. This article summarizes the physiological effects, pharmacological effects and related signal pathways of GPR124 published in the field of cardiovascular and cerebrovascular diseases published in recent years, in order to provide a reference for the study of the role of GPR124 in the occurrence and development of diseases.
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    Application of adipose-derived stem cells in treatment of neurodegenerative diseases: A review

    Fang-Fang LiuHao-Fei FanQing-Man LiFeng Guo...
    53-58页
    查看更多>>摘要：Neurodegenerative diseases are a class of progressive degenerative diseases that mainly include neuronal function decline and loss, such as Alzheimer's disease(AD); Cerebral troke; Parkinson's disease (PD); etc. These diseases are difficult to cure and seriously affect people's quality of life. Adipose derived stem cells (ADSCs) are derived from mesenchymal stem cells isolated and purified from adipose tissue. They have the characteristics of multidirectional differentiation and are widely used in the treatment of neurodegenerative diseases. Therefore, the use of ADSCs to treat this type of disease has potential application value. This article discusses the role of ADSCs in neurodegenerative diseases and their regeneration characteristics, and discusses the current clinical applications and future challenges of ADSCs, in order to provide references for the clinical application of ADSCs.
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    • NETL
    • NSTL
    • 万方数据