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海南医科大学学报（英文版）
海南医科大学学报（英文版）

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海南医科大学学报（英文版）/Journal Journal of Hainan Medical University
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    Plasma metabolomics study of Buyang Huanwu Decoction improving learning andmemory in D-gal model mice based on GC-MS

    YU Ming-huiXUE AoZHAO De-pingXU Yan-ming...
    1-7页
    查看更多>>摘要：Objective: To study the protective effect of Buyang Huanwu Decoction on the learning and memory ability of D-gal induced aging mice using GC-MS metabolomics method. Methods: Twenty-four three-month-old kunming animals were selected as experimental samples and randomly divided into control group, model group and Buyang Huanwu Decoction according to their body weight. The memory level of experimental animals was detected by novel body recognition test, and the neuron structure of experimental animals was detected by HE staining. The plasma of each group of experimental animals was quantitatively analyzed by gas chromatography-mass spectrometry, and the important metabolites and main metabolic pathways in the process of pathological changes were traced by using plasma metabolism. Results: In HE staining, compared with blank group, hippocampal neurons in model group were disordered and morphologically abnormal. Compared with the model group, the hippocampal neurons in Buyang Huanwu Decoction group arranged regularly and had normal morphology. Compared with blank group, the index of new object recognition in model group was significantly decreased (P<0.05); Compared with model group, the new object recognition index of Buyang Huanwu Decoction group was significantly increased (P<0.05). Five different metabolites of AD were identified by GC-MS, which were L-pyroglutamate, lysine, pyrophosphoric acid, creatinine and α-lactose. Conclusion: Buyang Huanwu Decoction has a significant effect on D-gal model mice, and can effectively improve their learning and memory level. The mechanism may be related to glutathione metabolism and aminophyl biosynthesis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    The effects of Yishen Qutong granula on pain sensitization and bone destruction of rats with bone cancer pain

    SONG Hong-liFENG Yong-liWANG Yao-hanFENG Li...
    8-14页
    查看更多>>摘要：Objective: To study the effects of Yishen Qutong granula on pain sensitization and bone destruction of rats with bone cancer pain. Methods: Walker256 cells were passaged in ascites and injected into the tibia of female Wistar rats to prepare the bone cancer pain model. On the 14th day after model establishment,60 rats were randomly divided into model group, sham-operated group, Yishen Qutong granula low, middle, high dose group and tramadol hydrochloride positive control group. After continuous administration for 14 days, the mechanical pain threshold, thermal threshold and weight-bearing difference of both hind limbs were observed. Results: Compared with the model group, Yishen Qutong groups increased the mechanical pain threshold, thermal pain threshold and reduced the weight difference of both hind limbs (P<0.05). Compared with the positive drug group, there was no significant difference in increasing the mechanical pain threshold and thermal pain threshold of rats in the medium dose group of Yishen Qutong (P>0.05), and Yishen Qutong granula significantly reduced the weight-bearing difference of hind limbs in all groups (P<0.05). Conclusion: Yishen Qutong granula can relieve pain sensitization and alleviate bone damage in rats with bone cancer pain.
      原文链接:
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    • 万方数据
    • 维普

    Study on metabonomics in patients with acute coronary syndrome based on LC-MS technology

    YU PengYANG Zhe-junSHI Rui-jieSUN Wei-xin...
    15-23页
    查看更多>>摘要：Objective: To investigate the plasma metabolic components of patients with unstable angina(UA) and acute myocardial infarction(AMI), and to screen potential biomarkers and explore possible biological mechanisms in order to provide reference for early evaluation of acute coronary syndrome. Methods: Plasma samples from patients with UA and AMI were collected to obtain their general information, and the metabolites were detected by LC-MS technology. Combined with univariate statistical analysis, the significantly different metabolites and their pathways were further determined by partial least squares discriminant analysis and orthogonalized partial least squares discriminant analysis models. Results: A total of 33 samples from UA group and 47 samples from AMI group were included for testing. Then 54 metabolites and 20 pathways were found to be significantly different between them. Metabolites such as pantoprazole, acetylcarnitine, palmitoyl ethanolamide, betaine, caprylic acid, bilirubin, histidine, oleic acid, citrate, vanillin can be used as potential biomarkers to distinguish them. The pathways include ABC transporters, aminoacyl-tRNA biosynthesis, central carbon metabolism in cancer and so on. Conclusion: There are significant differences in the plasma metabolic components of UA and AMI. Studies related to metabolomics may guide significance for exploring the biological nature of ACS.
      原文链接:
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    • NSTL
    • 万方数据
    • 维普

    Mechanism of PD‑1/PD‑L1 signaling pathway regulating Treg/Th17 in the occurrence and development of preeclampsia

    WANG LiCHEN Xiao?juLIN QingZHENG Lin?mei...
    24-29页
    查看更多>>摘要：Objective: To detect the expression levels of programmed cell death 1 (PD‑1) and its ligand PD‑L1, regulatory cell (Treg) and T helper cell 17 (Th17) specific nuclear transcription factors forkhead box protein 3 (Foxp3) and the retinoic acid‑related orphan nuclear receptorgammat (RORγc) in the placenta of normal pregnant women and in preeclamptic (PE) women, and to study the relationship between their differential expression and the development of PE. Methods: Between March 2021 and March 2022, 40 patients with pre‑eclampsia who were treated at the Obstetrical Department in Hainan Hospital were selected, including 20 cases of mild (PE) and 20 cases of severe (sPE), and 20 normal pregnant women with singleton cesarean section in the same period were selected to collect placental tissue. PD‑1, PD‑L1, Foxp3 and RORγc in placenta were detected by qRT‑PCR and Western blot. Results: (1) The results of qRT‑PCR assay showed that the mRNA expression of PD‑1, PD‑L1, Foxp3 protein decreased significantly compared with the control group. The changes in PD‑1, PD‑L1 and Foxp3 became more obvious as the disease progressed (P<0.05); The mRNA expression of RORγc increased significantly compared with the control group. The changes of RORγc mRNA became more obviousas as the disease progressed (P<0.05);(2)The results of Western blot assay show, compared with the normal group, the protein expression of PD‑1, PD‑L1, Foxp3 mRNA decreased significantly. With the aggravation of the disease, the changes of PD‑1 and PD‑L1 became more obvious, with significant difference (P<0.05); Compared with the normal group, the protein expression of RORγc increased significantly. With the aggravation of the disease, the changes of RORγc protein became more obvious with significant difference (P<0.05). Conclusion: PD‑1/PD‑Ll participates in the occurrence and development of preeclampsia by regulating Treg/Th17 immune balance.
      原文链接:
    • NETL
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    • 万方数据

    Study on the relationship between psoriasis vulgaris and GC gene in Hainan Han nationality based on target gene capture sequencing

    LIU KunLIU Jun-linYUAN Cheng-daLU Chun-wan...
    30-35页
    查看更多>>摘要：Objective: To investigate the relationships between GC gene polymorphisms and psoriasis vulgaris. Methods: A total of 101 patients with psoriasis vulgaris and 79 healthy controls were enrolled into this study, and they were all of Han nationality from Hainan province. The target gene capture sequencing method was used to sequence the full length of the GC gene and its 2kb upstream and downstream regions. SNP-based association analysis was performed under four genetic modes in SNPs with minimum allele frequency greater than 1％ and the P value of Hardy-Weinberg equilibrium test in the control group is greater than 0.05. Bioinformatics methods were used to predict the impact of risk SNP on gene function. Results: A total of 94 SNPs were detected, of which 93 met the inclusion criteria. SNP-based association analyses showed that 21 SNPs (16 in introns, 2 in exons, and 3 in Untranslated Regions) were susceptible to psoriasis vulgaris in at least one genetic mode (OR=0.289-2.295, 95％CI=0.048-12.670, P<0.05). Bioinformatic prediction indicates that rs4588, located in the exon 11, was a non-synonymous mutation and can convert threonine to lysine (SIFT Score=0.481,SIFT Score Pred=T). rs4752 A>G located in the exon 8 was a synonymous mutation and did not cause amino acid change. Conclusion: GC gene is associated with the susceptibility of psoriasis vulgaris in Hainan Han ethnic group.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Application of SOAT1 combined with multiple markers in the auxiliary diagnosis of hepatocellular carcinoma

    ZHENG JingWEI ShangDENG Hui-ziNIU Hai-yan...
    36-41页
    查看更多>>摘要：Objective: To explore the expression of sterol oxygen-acyltransferase 1 (SOAT1) in hepatocellular carcinoma tissues and to evaluate the application value of its combination with various markers (HSP70, GS, CD10, CD34) in the diagnosis of hepatocellular carcinoma. Methods: Totally 58 cases of liver cancer tissue and its corresponding adjacent tissue, and 12 cases of benign liver lesions tissue were collected, and tissue chips were made to detect the expressions of SOAT1, HSP70, GS, CD10 and CD34 (immunohistochemical staining method) which were analyzed by scientific method. Results: The expression of SOAT1 was up-regulated in hepatocellular carcinoma tissues, and the difference was statistically significant (P <0.01), and the expression level was closely related to the differentiation degree of hepatocellular carcinoma patients (P<0.05). SOAT1 was correlated with the expression of HSP70, GS, and CD34, with statistical significance (P<0.01). Positive percent agreement for the hepatocellular carcinoma was 100.00％ for CD34, 89.66％ for GS, 82.75％ for HSP70, 63.79％ for CD10, and 63.79％ for SOAT1; negative percent agreement (paracancerous tissues) was 100％ for SOAT1, 98.27％ for CD10, 96.55％ for HSP70, 34.48％ for CD34, and 5.17％ for GS; Negative percent agreement (benign tissues) was 100％ for SOAT1, 83.34％ for CD10, 100.00％ for HSP70, 8.33％ for CD34, and 0％ for GS, respectively. Taking the positive expression of any three markers as the diagnostic criteria, 39 of the 58 cases of hepatocellular carcinoma could be diagnosed, and the diagnostic rate was 67.24％ (SOAT1 was not included in the list of markers). If SOAT1 is included, 48 of the 58 hepatocellular carcinoma cases could be diagnosed, and the diagnosis rate could be increased to 82.76％. Conclusions: The expression level of SOAT1 was significantly up-regulated in hepatocellular carcinoma, and the expression level was higher in poorly differentiated HCC, suggesting that SOAT1 can be used as one of the indicators for the diagnosis and prognosis of hepatocellular carcinoma. Among the five markers above, CD34 and GS were more sensitive, while the specificity is better for SOAT1, CD10 and HSP70. The combined application of SOAT1 with HSP70, GS, CD10 and CD34 has certain application value in the diagnosis of hepatocellular carcinoma.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Clinical characteristics of 72 cases with neuromyelitis optical associated optic neuritis

    HAN Meng-yuQIN Ya-liJIAO Yu-juanJIAO Jin-song...
    42-49页
    查看更多>>摘要：Objective: To analyze the clinical characteristics of neuromyelitis optical associated optic neuritis (NMO-ON) patients, and to provide reference and basis for the prevention and treatment accordingly. Methods: The medical records of 72 NMO patients with ON as the first clinical manifestation in China-Japan Friendship Hospital from January 2016 to December 2019 were retrospectively analyzed and summarized, including general information, morbidity characteristics, course of disease, comorbid diseases, immunological tests, treatment response and prognosis, etc. Results: Totally 72 NMO-ON patients had a median age of 33 years. The ratio of male to female is about 1:5.54; The median course was 67 months, mainly "relapse-remission". Totally 61.11％ patients were successively involved in both eyes, the median incidence of ON was 2 times, and the median time of the second onset of ON was 3 months. The 1-year and 3-year recurrence rates were 55.56％ and 73.61％, respectively. Around 91.67％ of the patients had the onset of ON alone, and 81.94％ of the patients had monocular involvement. About 19.44％ patients were associated with inducement, the most common was upper respiratory tract infection; 15.28％ patients were associated with systemic immune diseases, most commonly associated with Sjogren's syndrome and thyroid diseases and 75.64％ patients had first visual acuity less than 0.1, aquaporin-4 immunoglobulin G (AQP4-IgG) status (P=0.032, OR =2.55) and onset age (P=0.037, OR=3.93) were independent risk factors for first visual acuity. Up to the last follow-up time, the rate of unilateral blindness was about 48.61％, and the median of unilateral blindness ON was 2 times. Other nervous system involvement occurred in 73.61％ of patients, and spinal cord (61.11％) was the most common site of recurrence. Serum AQP4-IgG was positive in 80.00％ (48/60) of patients. A total of 18 cases (25.00％) were associated with other systemic immune antibodies, most commonly associated with ANA antibody positivity. Conclusions: The first onset of NMO-ON patients is mostly ON alone, with unilateral involvement and high incidence in young and middle-aged women. Bilateral optic nerve involvement and repeated recurrence are common in the long course of disease. AQP4-IgG status and onset age are independent risk factors affecting the visual function of NMO patients for the first onset, and most patients have positive AQP4-IgG serum. Some patients are associated with systemic immune diseases represented by Sjogren's syndrome and thyroid disease, which are at high clinical risk and require early diagnosis and treatment intervention.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Mechanism of total flavonoids in the treatment of rheumatoid arthritis based on network pharmacology

    LIU Xiao-yuCHEN Guang-yaoWU Zi-yuJIN Qi...
    50-58页
    查看更多>>摘要：Objective: To explore the mechanism of total flavonoids of Rhizoma Drynariae (TFRD) in the treatment of rheumatoid arthritis (RA) based on network pharmacology. Methods: The active components of TFRD were identified from the Traditional Chinese Medicines for Systems Pharmacology Database and Analysis Platform (TCMSP). Relevant targets of TFRD were predicted by TCMSP, PubChem, SwissTargetPrediction and STITCH database, and standard target names were obtained from Uniprot database. RA-related targets were retrieved from GenBank, GeneCards, DisGeNet and OMIM databases. The intersection genes were imported into STRING database to construct a protein-protein interaction (PPI) network, in order to predict the core proteins. GO functional annotation analysis and KEGG pathway enrichment analysis were then performed. A "drug-component-target-disease" regulatory network was constructed using Cytoscape 3.8.2, and the active components of TFRD were molecularly docked with the predicted core targets. Results: A total of 10 active compounds, including luteolin, naringenin and kaempferol, were screened out from TCMSP database, and 210 relevant targets were predicted. A total of 2009 RA-related targets were screened out, and 123 targets of TFRD-RA intersection targets were obtained. STAT3, MAPK1, MAPK3, AKT1, MAPK8, IL-6, TNF, MAPK14, IL-4, IL-2, VEGFA, IL-1β and MAPK9 may be the key targets of TFRD in the treatment of RA. GO functional enrichment analysis suggested that transcriptional regulation and cytokine activity regulation may play an important role in TFRD in RA treatment. KEGG pathway enrichment analysis suggested that PI3K/AKT, IL-17, TNF-α, T cell receptor transduction and regulation of Th17 cell differentiation may play an important role in the treatment of RA by TFRD. The regulatory network suggested that luteolin and naringenin may be the key components of TFRD in the treatment of RA, which also had good affinity with the core targets AKT1, PI3K and STAT3 by molecular docking, which may further affect their phosphorylation by changing the molecular conformation. Conclusion: TFRD may inhibit the inflammatory response of RA by regulating inflammation-related cytokines and related conduction pathways, and PI3K/AKT pathway may be an important pathway of TFRD in the treatment of RA. Luteolin is the key component of TFRD in the treatment of RA, and may play a vital role in the downstream pathway by inhibiting the conformation of related core proteins and thereby regulating phosphorylation.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Tanreqing injection auxiliary in the treatment of heart failure with pulmonary infection: A systematic review

    YAN Long-meiZHANG Jing-chunAI Yu-zhenXING Ya-xuan...
    59-65页
    查看更多>>摘要：Objective: To systematically evaluate the clinical efficacy and safety of Tanreqing injection in the treatment of heart failure complicated with pulmonary infection. Methods: The database of CNKI, SinoMed, VIP full text database, Wanfang database, Cochrance Library, Web of Science and PubMed were searched. The retrieval time was from the inception to August 2021. Clinical randomized controlled trial of Tanreqing injection in the treatment of heart failure complicated with pulmonary infection was collected, and two researchers independently screened the document data. Meta-analysis was performed using RevMan 5.4.1 software. Results: A total of 10 documents were included, including 862 cases of heart failure complicated with pulmonary infection, including 431 cases in the test group, and 431 cases in the control group. The Meta analysis showed that compared to the control group, the test group increased clinical efficiency [OR=4.56, 95％ CI(2.79, 7.52), P<0.00001], reduced the value of C-reactive protein [MD =-7.55, 95％ CI (- 11.40, -3.69), P=0.0001], reduced the time required to correct heart failure [OR=-4.04, 95％ CI (-4.59, -3.49), P<0.00001], reduced the number of days of the average hospitalization [MD =-4.78, 95％CI (-6.67, -2.89), P<0.00001], and there were no statistically significant differences in the incidence of adverse reactions. Conclusion: Tanreqing injection, as an auxiliary treatment for heart failure complicated with pulmonary infection, has significantly effective effect on improving efficiency. Tanreqing injection has a certain advantage in reducing C-creative protein values, shortening the time of correcting heart failure, and reducing the number of days of the average hospitalization, and the adverse reactions are smaller. However, the overall quality of the included studies is low, and more high-quality randomized controlled trials are needed to increase the evidence-based basis.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Research progress on modern pharmacological action of Radix bupleuri

    LI Yue-yangLEI Gen-pingDONG ShengFENG Guan-qiang...
    66-72页
    查看更多>>摘要：Radix bupleuri is the dried root of Umbelliferae plants Bupleurem chinense DC. or Bupleurem scorzonerifolium Willd., which are widely distributed in Northeast China, North China, Northwest China, East China, Hubei, Sichuan and other places. The plant roots are excavated in spring and autumn and dried with removing stems and leaves and sediment. Radix bupleuri, one of the oldest and most commonly used Chinese herbal medicine, has a long history of application, with high medicinal value. Modern pharmacological studies have shown that Radix bupleuri has pharmacological effects such as anti-cancer, anti-depression, anti-inflammation, heart protection, liver protection, and kidney protection, and mainly plays an role in regulating apoptosis signaling pathway, inflammation signaling pathway, neuroendocrine system, oxidative stress signaling pathway, and fibrosis signaling pathway. This paper reviews the modern pharmacological effects of Radix bupleuri in recent years, in order to provide reference for better research, development and rational utilization of Bupleurum resources.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普